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Exploring injection principles

1-23

The injection valve switches to the Inject position, and the sample loop
becomes part of the flow path for the HPLC mobile phase. Sample enters
the column, and analysis starts.

After each injection, a wash routine of programmable volume occurs.

Wash station

Buffer tubing

Syringe

Needle

Sample loop

INJECT

Column

Pump

Sample

Содержание 2707

Страница 2: ...me of publication In no event shall Waters Corporation be liable for incidental or consequential damages in connection with or arising from its use Trademarks Waters is a registered trademark and Empo...

Страница 3: ...encounter in this document or to suggest ideas for otherwise improving it Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and u...

Страница 4: ...e Adoption of Analytical Methods in the Clinical Chemistry Laboratory American Journal of Medical Technology 44 1 pages 30 37 1978 This protocol addresses good operating procedures and the techniques...

Страница 5: ...and applications The optional heating cooling module accommodates a wide range of samples from heat labile biological samples to viscous polymer samples Quality control Routinely run three quality co...

Страница 6: ...vi...

Страница 7: ...overfill injections 1 13 Partial loop injections 1 18 Using well plates and vial trays 1 24 2 Preparing the Autosampler 2 1 Preparing for installation 2 2 Setting up the instrument 2 2 Plumbing the i...

Страница 8: ...enance basics 3 2 Contacting Waters Technical Service 3 2 Heeding maintenance considerations 3 3 Safety and handling 3 3 Proper operating procedures 3 4 Cleaning the autosampler 3 4 Removing or replac...

Страница 9: ...ions 4 2 Changing the rear panel interface connections 4 3 Understanding power surges 4 4 Understanding the status LED 4 4 Conducting analytical troubleshooting 4 4 A Safety Advisories A 1 Warning sym...

Страница 10: ...rials of Construction and Compliant Solvents B 1 Preventing contamination B 2 Naming solvents for mobile phase preparation B 2 C Specifications C 1 Outlining general specifications C 2 Outlining prep...

Страница 11: ...1 1 1 Understanding Operating Principles Contents Topic Page Describing the autosampler 1 2 Identifying options 1 5 Exploring injection principles 1 6 Using well plates and vial trays 1 24...

Страница 12: ...equires little bench space High resolution syringe control for high precision injections Interchangeable fixed volume sample loops Variable volume partial loop injection capability Optional sample coo...

Страница 13: ...1000 L buffer tubing The instrument uses standard high or low well plates or vial trays The sampling compartment can house two well plates in any combination Exception The sampling compartment cannot...

Страница 14: ...se items appear on the instrument s rear panel ETHERNET I O RJ 45 Ethernet connection 9 pin male connector I O connections Warning label On off switch Fuse drawer Power connector Sample compartment co...

Страница 15: ...user installable options Biocompatible sample flow path and valve Inert sample needle Silco steel and biocompatible valve PEEK Prep kit for large volume sampling 2500 L syringe prep valve 10000 L sam...

Страница 16: ...ple aspiration For all injection modes the instrument method can enable loop injection with pressure assisted sample aspiration This option pairs an on board compressor with intelligent valve switchin...

Страница 17: ...d sample needle Understanding syringe and buffer tubing The instrument has two syringe sizes 500 L standard 2500 L included in the prep kit With standard 1000 L buffer tubing and the standard 100 L sa...

Страница 18: ...me for the needle The minimum flush volume of 30 L is recommended you can program smaller flush volumes but they decrease performance This is the full loop injection sequence 1 The injection valve mov...

Страница 19: ...The syringe dispenser aspirates the flush volume 30 L default from the sample well or vial to fill the sample line with sample and remove wash solvent Sample Buffer tubing Syringe Needle Sample loop...

Страница 20: ...ding Operating Principles 3 The injection valve switches to the Load position placing a sample plug at the sample loop inlet TP03046 Sample Syringe Needle Sample loop LOAD Column Pump Buffer tubing Ai...

Страница 21: ...he loop as follows 3 loop volume for loops 100 L 2 loop volume for loops 100 to 500 L 1 5 loop volume for loops 500 L 5 The injection valve switches to the Inject position and the sample loop becomes...

Страница 22: ...cted can minimize sample dilution caused by dispersion during aspiration With a standard needle recommended flush volumes are 30 L for injections that incorporate air segments and 35 L for injections...

Страница 23: ...um flush volume of 30 L is recommended This is the partial loop needle overfill injection sequence 1 The injection valve moves to the Inject position and the sample needle and air needle enter the via...

Страница 24: ...iples 2 The syringe dispenser aspirates the flush volume 30 L default from the sample vial to fill the sample line with sample and remove wash solvent Sample Syringe Needle Sample loop INJECT Column P...

Страница 25: ...principles 1 15 3 The injection valve switches to the Load position placing a plug of sample at the beginning of the sample loop TP03068 Buffer tubing Sample Syringe Needle Sample loop LOAD Column Pu...

Страница 26: ...1 16 Understanding Operating Principles 4 The programmed injection volume is aspirated into the sample loop Sample Buffer tubing Syringe Needle Sample loop LOAD Column Pump...

Страница 27: ...sh volume but not injected can minimize sample dilution caused by dispersion during aspiration With a standard needle recommended flush volumes are 30 L for injections that incorporate air segments an...

Страница 28: ...or injection volumes 10 L Recommendation Because wash solvent injects with the sample in partial loop mode consider the effect on the chromatography In general for reverse phase chromatography match t...

Страница 29: ...principles 1 19 2 The syringe dispenser aspirates a plug of wash solvent from the wash station filling the sample line with transport liquid Wash station Buffer tubing Syringe Needle Sample loop INJEC...

Страница 30: ...ding Operating Principles 3 The needle moves from the wash station to the sample vial The injection valve switches to the Load position Wash station Buffer tubing Syringe Needle Sample loop LOAD Colum...

Страница 31: ...Exploring injection principles 1 21 4 The programmed injection volume of sample is aspirated from the sample vial TP03052 Wash station Buffer tubing Syringe Needle Sample loop LOAD Column Pump Sample...

Страница 32: ...erating Principles 5 The sample needle moves back to the wash station and a second plug of wash solvent is aspirated The sample enters the loop Wash station Buffer tubing Syringe Needle Sample loop LO...

Страница 33: ...ct position and the sample loop becomes part of the flow path for the HPLC mobile phase Sample enters the column and analysis starts After each injection a wash routine of programmable volume occurs W...

Страница 34: ...ir expansion during exchange from the sample vial well to the transport position Using well plates and vial trays The instrument accommodates the following well plates and vial holders 96 well tall an...

Страница 35: ...ls Caps with PTFE or preslit PTFE silicone septa For samples in low volume inserts PTFE silicone caps or cap mats For samples in plates Polypropylene or PTFE silicone cap mats heat sealed microtiter p...

Страница 36: ...1 26 Understanding Operating Principles...

Страница 37: ...ng for installation 2 2 Setting up the instrument 2 2 Plumbing the instrument 2 3 Making Ethernet connections 2 11 Controlling I O connections 2 12 Connecting to the electricity source 2 13 Installing...

Страница 38: ...damage or discrepancy when inspecting carton contents immediately contact the shipping agent and your local Waters representative If you are in the USA or Canada report damage and discrepancies to Wa...

Страница 39: ...igned to move more slowly when the instrument s front cover is removed or the door is open Caution Extreme environmental conditions can impede instrument performance so choose an indoor operating loca...

Страница 40: ...2 4 Preparing the Autosampler To open the instrument door 1 Grasp the door handle 2 Gently pull the handle toward you and push it upward until it is horizontal...

Страница 41: ...r into the cabinet Tip To access components more easily remove the instrument s cover To remove the instrument s cover 1 Simultaneously press the two black buttons on either side of the top of the ins...

Страница 42: ...paring the Autosampler When the cooling option is installed a cooling cover overlays the well plates To remove the cooling cover slide the cooling cover outward by pulling it gently toward you Cooling...

Страница 43: ...instrument s bottom One drains solvent and sample spills the other drains water from the cooler as well as sample spills and mobile phase leaks Fluid line connections Recommendation If air bubbles app...

Страница 44: ...attached to the injection valve at the factory Requirement Correctly connect these items HPLC pump blue to port 1 of the injection valve HPLC column red to port 6 of the injection valve Routing and co...

Страница 45: ...mobile phase pump to deliver 1 mL min of 100 methanol or acetonitrile for 10 minutes Recommendation Before connecting the column flush the system according to the following procedure 4 Route the red...

Страница 46: ...path clear ensure that the drain or waste tubes are untwisted Accommodating the wash solvent and syringe rinse To fill the wash solvent tubing 1 Fill a clean wash solvent bottle with wash solvent and...

Страница 47: ...mples of Empower select Wash Prime Needle to fill the tubes with liquid Tip To remove all air from the syringe and achieve maximal operation perform an extra wash Tip If bubbles form in the syringe on...

Страница 48: ...f the input becomes active during an analysis it has no effect until the next analysis is started Stop Inject Aborts the run immediately Explaining contact closure output Requirement Route an Inject S...

Страница 49: ...onnect to the electricity source Recommendation Use a line conditioner or an uninterruptible power supply UPS for optimum long term input voltage stability 1 Confirm that fuses and the voltage range o...

Страница 50: ...elect the Prep mode check box on the Volumes tab Then select the Use Prep Mode check box Tip For procedures on removing and replacing prep kit accessories see Chapter 3 Installing the biocompatibility...

Страница 51: ...rations 3 3 Cleaning the autosampler 3 4 Removing or replacing the injection valve rotor seal 3 5 Installing a sample loop 3 6 Replacing the sample needle 3 6 Adjusting needle position 3 8 Maintaining...

Страница 52: ...s for Waters locations worldwide Go to www waters com and click About Waters Worldwide Offices When you phone Waters Technical Service be prepared to provide the following information Error message if...

Страница 53: ...damaging electrical parts never disconnect an electrical assembly while power is applied to the instrument To completely interrupt power to the instrument set the power switch to Off and then unplug t...

Страница 54: ...void malfunctions resulting from the following conditions flush buffered mobile phase from the instrument before shutting it down Plugged solvent lines and flow cell Damaged instrument components Micr...

Страница 55: ...tubing from the valve Exception Keep the sample loop intact 2 Using a 2 Phillips screwdriver remove the two screws on either side of the valve body Tip The two removed screws secure the plastic valve...

Страница 56: ...of syringe and tubing Connect the loop between ports 2 and 5 of the injection valve When installing a loop of nonstandard volume from the Configure menu of the 2707 Console click Volumes and adapt set...

Страница 57: ...ndation Make sure the air seal encircles the needle 7 Using the needle connection nut tighten the needle assembly 8 Connect the other end of the needle connection tubing to port 4 of the injection val...

Страница 58: ...When using plates or holders whose dimensions do not comply with ANSI dimensions or other geometrical offsets use the Adjust Needle function Maintaining air needles The instrument uses six air needle...

Страница 59: ...deep wells Tip When deviating from standard settings use an optional needle type Recommendation Do not use needle pressurization for low well plates The sample needle punctures the seal to prevent va...

Страница 60: ...needle height in mm Nh Distance of the air needle point through the capmat or seal in mm minimum 2 mm Ac Ht Dw must be between 2 and 6 mm at which value you can calculate the protrusion length of the...

Страница 61: ...ndard needle height Ht 41 4 mm The following is true Dw 37 8 mm 41 4 37 8 3 6 is between 2 and 6 mm Cd 3 8 mm Protrusion length 41 4 3 8 6 0 2 0 29 6 Nh 6 0 mm standard Ac 2 0 mm minimum A 56 mm air n...

Страница 62: ...ects the tubing to port 4 of the injection valve see the preceding figure 5 Grasp the sample needle s tubing and withdraw the needle from the fitting 6 Begin removing the air needle by unscrewing the...

Страница 63: ...Replace Needle window click Reset Result The air needle returns to the home position 13 Clean the new needle by clicking Wash needle from the Control menu of the 2707 Console 14 If needed use the Adj...

Страница 64: ...rt of the prep kit To replace a syringe 1 From the Maintain menu of the 2707 Console click Replace Syringe Result The syringe plunger lowers 2 Unscrew the syringe from syringe valve Requirement Keep t...

Страница 65: ...ace Sample Syringe window click Reset Result The syringe moves to the home position and dispenses its contents to waste 10 Using the Control menu of the 2707 Console perform a standard wash routine Re...

Страница 66: ...lip Requirement Keep the top threaded end below the syringe valve 11 Raise the syringe and screw it firmly into the connector 12 In the Replace Sample Syringe window click Reset Result The syringe mov...

Страница 67: ...ger lowers 2 Unscrew the syringe from syringe valve Requirement Keep the connector in the valve intact 3 Disconnect the plunger from the syringe drive by sliding it straight back and out of the holdin...

Страница 68: ...an 2 turns 11 Slide the syringe valve downward out of the instrument 12 Remove the syringe valve 13 Remove the waste fitting at the rear of the valve 14 Attach the waste fitting of the new valve 15 In...

Страница 69: ...below the syringe valve 22 Raise the syringe and screw it firmly into the connector 23 In the Replace Sample Syringe window click Reset Result The syringe moves to the home position and dispenses its...

Страница 70: ...3 20 Maintaining the Autosampler If fuse problems recur contact Waters Service...

Страница 71: ...age Addressing instrument errors 4 2 Running diagnostic tests 4 2 Displaying the statuses of signal connections 4 2 Changing the rear panel interface connections 4 3 Understanding power surges 4 4 Con...

Страница 72: ...steps To initiate the mechanism homing steps that occur upon startup use the Control menu of the 2707 Console Displaying the statuses of signal connections By displaying the statuses of the I O signa...

Страница 73: ...ignal connection and then click the red or green LED symbol Tip The output signal indicates whether the selected signal connection is open or closed Pin number Cable color Signal connections Descripti...

Страница 74: ...l procedures to find the cause First determine whether the instrument or the rest of the system is causing the problem by doing the following Replace the valve by a manual injection valve to discrimin...

Страница 75: ...trol panel Leaking syringe If leakage occurs at the top of the syringe determine whether the syringe is mounted properly and that its gasket is in place If leakage occurs at the bottom of the syringe...

Страница 76: ...ect the buffer tubing from the syringe valve 6 Start a manual wash 7 If solvent flows from syringe valve inspect the buffer tubing if not check for overtightened connections throughout the flow path a...

Страница 77: ...des also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and statements that apply t...

Страница 78: ...ks include burn injuries electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying text identifies the spec...

Страница 79: ...g around such tubing Wear eye protection Extinguish all nearby flames Do not use tubing that is or has been stressed or kinked Do not expose nonmetallic tubing to incompatible compounds like tetrahydr...

Страница 80: ...l surfaces of the mass spectrometer when the instrument is in Operate mode To avoid non lethal electric shock make sure the instrument is in Standby mode before touching areas marked with this high vo...

Страница 81: ...When operating this device follow standard quality control procedures and the equipment guidelines in this section Warning Waters instruments can be used to analyze or process potentially hazardous s...

Страница 82: ...n oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems f hren...

Страница 83: ...e trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou n...

Страница 84: ...no rigonfiamento nei tubi non metallici riducendo notevolmente la resistenza alla rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi...

Страница 85: ...Warnings that apply to all Waters instruments A 9...

Страница 86: ...isque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes unter Umst nden nicht ordnungsgem funktionieren Attenzione l utente deve ess...

Страница 87: ...de la m me puissance afin d viter tout risque d incendie Vorsicht Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen Attenzione per una buona p...

Страница 88: ...prise avant d effectuer la maintenance de l instrument Vorsicht Zur Vermeidung von Stromschl gen sollte das Ger t vor der Wartung vom Netz getrennt werden Attenzione per evitare il rischio di scossa e...

Страница 89: ...ls These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor ter...

Страница 90: ...visories Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Keep upright Keep dry Fragil...

Страница 91: ...B 1 B Materials of Construction and Compliant Solvents Topic Page Preventing contamination B 2 Naming solvents for mobile phase preparation B 2...

Страница 92: ...S Systems part number 715001307 found on http www waters com Click Services and Support and then Support Center Naming solvents for mobile phase preparation Following are the most common ingredients u...

Страница 93: ...C 1 C Specifications Contents Topic Page Outlining general specifications C 2 Outlining prep version specifications C 6...

Страница 94: ...Dimensions Without cooling 300 mm W x 510 mm D x 360 mm H With cooling 300 mm W x 575 mm D x 360 mm H Weight Without cooling 19 kg With cooling 21 kg Maximum weight that can be placed on the instrumen...

Страница 95: ...cluding 300 L wash Analytical performance Injection modes Full loop partial loop needle overfill partial loop pressure assisted sample aspiration Reproducibility valid at 1 0 cP RSD 0 3 for full loop...

Страница 96: ...d Sample tray cooling Built in Peltier cooling Range 4 C to ambient 3 C Air temperature in sample compartment 4 C 2 C at temperature sensor Temperature at 80 relative humidity and 25 C ambient tempera...

Страница 97: ...ressure valve to syringe valve label 1000 L ETFE Tefzel 1275 mm 1 16 OD 1 0 mm ID Tubing syringe valve to wash solvent bottle PTFE 400 mm 1 8 OD 1 6 mm ID Tubing syringe valve to waste PTFE 400 mm 1 8...

Страница 98: ...ume determines the aspirated sample volume Dispenser syringe 2500 L syringe Injection volume 0 L 10000 L with 1 L increments Valve Valco 0 75 bore valve Sample loop 10 mL SS sample loop 1 8 tubing wit...

Страница 99: ...g procedures 3 4 contact closure output 2 12 contamination preventing B 2 cooling cover 2 6 cooling option 1 5 D diagnostic tests 4 2 E electrical symbols A 13 electricity source 2 13 equipment guidel...

Страница 100: ...lacement 3 6 sampling compartment 1 3 signal connections displaying 4 2 solvents use in mobile phases B 2 spare parts 3 4 status LED 4 4 symbols caution A 5 electrical A 13 handling A 14 warning A 2 s...

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