Exploring injection principles
1-7
The syringe aspirates the sample from a vial into the sample loop. Buffer
tubing between the syringe and the injection valve prevents syringe
contamination. Wash solvent serves to:
•
Remove sample from the buffer tubing and sample needle
•
Rinse the buffer tubing and sample needle
Understanding syringe and buffer tubing
The instrument has two syringe sizes:
•
500 µL (standard)
•
2500 µL (included in the prep kit)
With standard 1000-µL buffer tubing and the standard 100-µL sample loop,
injection volume ranges are as follows:
•
Full loop – 100 µL
•
Partial loop needle overfill – 0 to 50 µL
•
Partial loop – 0 to 27 µL
Maximum injection volumes are calculated as follows:
•
Full loop – Loop volume
•
Partial loop needle overfill – Half the loop volume
•
Partial loop – (Loop volume - [3 × needle volume])/2
Содержание 2707
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