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Operating Instructions | symphony 5.3 A – 06/2009

1

Important safety informations

1

Important safety informations

12

When using such disinfectants, avoid open flames or exposure
to excessive heat during the entire disinfection process!
• Use such disinfectants only in adequately ventilated rooms.
• After the disinfectant has been allowed to react, wipe the

cleaned device components thoroughly dry!

• Observe safety regulations to avoid fire and/or explosion

hazard caused by alcohol-containing disinfectants!

Water supply:

The water tray of the work space can hold up to 3.0 l of proces-
sed water. When the running operation, always keep a suf-
ficient quantity of processed water of the following quality
available:
• demineralized and distilled or autoclaved for sterilization,
or
• completely deionized and distilled or autoclaved for sterili-

zation.

• When the water level falls below the lower limit, refill

water!

1.6 Intended purpose of the device

1.6.1 Correct use

The CO

2

incubator is a laboratory device for preparing and cultivating cell

and tissue cultures. The device allows the simulation of the special physio-
logical ambient conditions for these cultures due to the exact control of:

Control features

• temperature,
• CO

2

content,

• an increased relative humidity.

The device has been designed for installation and operation in the follo-
wing fields of application:

Fields of application

• Laboratories for cytobiological and biotechnological experiments of

safety levels L1, L2, and L3.

• Medical-microbiological laboratories in accordance with DIN 58 956.
• Laboratories in the central area of clinics and hospitals.

Gas supply

The CO

2

required for the incubator is supplied to the device from a separate

gas supply system, either from gas cylinders or from a central pressurized
gas container.
The layout of the gas supply system must ensure that the operating pres-
sure of the gas supply lines can be set to a range between 0.8 bar (min.) to
1 bar (max.) and that the pressure cannot be changed.
Depending on the capability of the gas supply system, several devices may
be connected to one gas cylinder.
The CO

2

incubator is suited for continuous operation.

1.6.2 Incorrect use

Safety levels

Do not use cell or tissue cultures in the device that are not in accordance
with the regulations of safety levels L1, L2, and L3.

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Содержание symphony 5.3 A

Страница 1: ...Valid 06 2009 50111308 B Operating Instructions CO2 Incubator symphony 5 3 A symphony 17 o ee 50 O O C 1 vwn VWR __ ...

Страница 2: ... the VWR This regulation does not apply to reproductions for in plant use Trademarks All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers VWR International 1310 Goshen Parkway West Chester PA 19380 USA Technical Support When contacting the Technical Support please have the following information available Model Number Serial Number Date P...

Страница 3: ...2 Delivery 14 2 1 Packaging 14 2 2 Components standard equipment 14 2 3 Acceptance inspection 14 3 Installation of the device 15 3 1 Ambient conditions 15 3 2 Room ventilation 15 3 3 Space requirements 16 3 4 Transport 16 3 5 Stacking 17 3 6 Modifications 17 4 Description of the device 18 4 1 Views of device 18 4 2 Safety devices 20 4 3 Work space atmosphere 20 4 4 Sensor system 21 4 5 Door switch...

Страница 4: ...disinfection 47 9 1 Decontamination procedures 47 9 2 Wipe Spray disinfection 47 9 3 90 C decontamination routine optional 50 9 3 1 90 C decontamination routine procedure 51 9 3 2 Activating the 90 C decontamination routine 52 9 3 3 Cancelling the 90 C decontamination routine 52 10 Maintenance 53 10 1 Inspections and checks 53 10 2 Service intervals 53 10 3 Preparing the temperature calibration 53...

Страница 5: ...Operating Instructions symphony 5 3 A 06 2009 Contents 5 14 Device log 66 15 Certificate of decontamination 67 ...

Страница 6: ...lation removal 26 Fig 11 Shelf support sheet insert installation 27 Fig 12 Gas pressure hose installation 28 Fig 13 Power supply connection 29 Fig 14 RS 232 interface 29 Fig 15 Example of a connection scheme 33 Fig 16 Power switch Operating panel 37 Fig 17 Removing blower wheel and cover 49 Fig 18 90 C decontamination routine procedure 51 Fig 19 Temperature calibration 54 Fig 20 CO2 calibration 56...

Страница 7: ...protective measures apply while specific agents are used which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authori zed expert personnel 1 2 Applicability of the instructions The contents of the operating instructions are subject to change without further notice Basic language Concerning translations into foreign languages the US...

Страница 8: ...ous injury 1 CAUTION Indicates a hazardous situation which if not avoided could result in minor or moderate injury NOTICE Indicates a situation which if not avoided could result in property damage NOTE Is used for applicational hints and useful information 1 4 2 Pictographs used in the operating instructions Pictographs point to concrete facts of a safety message or a property damage message Elect...

Страница 9: ...bator is started up and when the device is operated NOTE Personnel instruction Personnel operating devices with CO2 supply must be instruc ted about the particularities in the handling of CO2 before star ting their work Correct operation of pressurized gas containers and gas supply systems Obligation to report damages and shortcomings in CO2 sup ply lines Measures to be taken in case of accidents ...

Страница 10: ...ation opening must not be extended or redirected Access port If the access port is not used it must be capped during ope ration Gas quality CO2 must be of the following qualities Purity 99 5 min medical grade 1 CAUTION Electric shock Contact with current carrying components may cause a lethal electric shock Before connecting the device to the power supply check plug and connection line for damage ...

Страница 11: ...he user must clean and disinfect the work space in accordance with the hygiene regulations set forth by the operator to protect the cultures NOTICE Incompatible cleaning agents Some device components are made of plastic Solvents may dissolve plastics Powerful acids or bases may cause embrittle ment of the plastics For cleaning the plastic components and surfaces do not use hydrocarbon containing s...

Страница 12: ...res The device allows the simulation of the special physio logical ambient conditions for these cultures due to the exact control of Control features temperature CO2 content an increased relative humidity The device has been designed for installation and operation in the follo wing fields of application Fields of application Laboratories for cytobiological and biotechnological experiments of safet...

Страница 13: ... voltage fluctuations not to exceed 10 of the nominal voltage Installation Category II1 Pollution Degree 22 Class of Equipment I 1 Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity sup ply and is overvoltage protection means For example in Cat II which is the categor...

Страница 14: ...k After the device has been delivered check the delivery immediately for completeness possible damages If damages are detected or if components are missing please contact the carrier and vendor immediately Delivery CO2 incubator with single glass door and with continuous shelves standard version Shelf 3 Shelf support post 4 Shelf support rail 6 Insert for pressure compensation opening 1 Plug for p...

Страница 15: ...ld not be used near the loca tion of the CO2 incubator symphony 5 3 A 3 2 Room ventilation When CO2 is supplied the work space of the incubator is slightly pressuri zed The pressure is released through the pressure compensation opening into the operating room As the pressure compensation and any opening of the glass door gas tight screen during the operation of the device will release very small q...

Страница 16: ...stand even if the device is installed near the floor The height of the floor stand should not be less than 200 mm Several floor stands and carriers are available as opti ons from VWR part numbers see Section 11 1 Parts and accessories NOTE Accessibility of the devices To ensure the accessibility for care and maintenance works keep larger side and rear distances 3 4 Transport Fig 2 For transport do...

Страница 17: ... the devices are placed onto mobile racks ensure that the rollers 3 are secured by an arresting device during operation For reasons of stability the rollers should be oriented to the front NOTE Transporting stacked devices The stacking elements are anot connecting elements Therefore the transport of stak ked devices on sloped surfaces is not allo wed 3 6 Modifications NOTE Modifications Retrofits ...

Страница 18: ...nsation opening with insert 7 Measuring opening 8 Outer door 9 Door handle 10 Outer door seal replaceable 11 Stand height adjustable 12 Nameplate 13 Power switch 14 Support rail 15 Shelf 16 Latch glass door 17 Support hook for shelf 18 Access port with plug 19 Glass door seal replaceable Rear view Description rear side 20 Stacking elements 21 Pressure compensation opening 22 Access port Ø 42 mm 23...

Страница 19: ...ctions symphony 5 3 A 06 2009 4 Description of the device 4 Description of the device 4 Description of the device 19 12 2 10 11 21 23 20 20 20 22 24 13 14 16 17 18 19 15 2 5 4 3 1 8 7 6 9 Fig 4 General views of device ...

Страница 20: ...ing system controls the incubation temperature from this tempe rature threshold up to 55 C The principle of air jacket heating and the addi tional separate heating of the outer door minimize the risk of condensate formation on the side walls at the ceiling of the work space and at the glass door Relative humidity The water tray of the work space can hold 4 5 liters of processed water The heating o...

Страница 21: ...nd CO2 supply The blower intermixes the supplied gases and ensure uniform temperature distribution within the work space The thermal protection has been preprogrammed at the factory and cannot be changed It protects the stored cultures from overheating If the temperature is exceeded by more than 1 C the thermal protection responds and the work space temperature is automa tically reduced to the set...

Страница 22: ...th a separation rate of 99 97 for a particle size of 0 3 μm HEPA filter quality Label Fig 7 The label 2 contains information about gas supply an alarm contact terminal legend and notes about the electrical fusing of the device RS 232 interface Fig 7 Via the RS 232 interface 3 the incubator can be connected to the serial interface of a PC This con nection allows the computer aided acquisition and d...

Страница 23: ...aning and manual disinfection works Water reservoir Fig 8 The water reservoir 2 is integral to the internal chamber floor and inclines toward the rear The embossing in the water tray is used to indicate mini mum level 3 and the maximum level 4 Heating system Fig 8 An air jacket heating is used for heating the work space The arrangement of the heating elements ensures that condensate formation abov...

Страница 24: ...in the operating room NOTE Operating conditions When accessories are to be operated in the work space of the CO2 incubator the ambi ent condition requirements must be obser ved see table below The energy introduced into the work space affects the beginning of the temperature control range When additional heating sources are introduced into the work space condensa tion e g at the glass door may occ...

Страница 25: ...is created for 9 hours at a temperature of 90 C The effectiveness of the decontamination routine has been tested and cer tified by independent institutes Information about these tests is available at request from VWR The entire program run of the decontamination routine will take approx 25 hours After the run has been completed the device must be reactivated using the autostart routine NOTE Therma...

Страница 26: ... shelf system The support rails are secured using spring pressure After the support hooks have been inserted into the rail the shelves are pushed onto the support hooks A Support rail installation removal Fig 10 The support rails are held at the sides by embossing 2 and 5 and secured by the embossing 1 and 6 The support rails marked with are inser ted at the rear panel of the device with the locks...

Страница 27: ...ion 5 facing the rear panel of the device The tilt protection 5 is also used as a guide for the shelf 2 Slightly raise shelf so that the withdrawal stop 6 can be routed over the shelf supports 3 Make sure that the shelf supports are positioned in the two tilt protectors in a way that it can move freely Levelling the device 1 Position a bubble level on the center shelf 2 Rotate the adjustable devic...

Страница 28: ...pensation opening To ensure permanent pressure compensa tion the pressure compensation opening must not be connected to an exhaust air system The pipe of the pressure compen sation opening must not be extended or redirected Access port If the access port is not used it must be capped during operation CO2 connection A Fig 12 The gas supply from the gas supply system to the device is achieved using ...

Страница 29: ... at the front of the device If the ratings given for voltage V and current A are not cor rect the device must not be connected to the power supply 2 Fig 13 Connect the connector for non heating appliances 2 to the sokket 1 at the control box of the device 3 Connect the grounding plug 3 of the power supply cable to a correctly grounded and fused socket 4 Make sure the power supply cable is not subj...

Страница 30: ...ation 0001 0001 11H for CO2 Device response for temperature and CO2 Data Nominal value x 10 2 bytes integer Nominal value 4 bytes floating point number Internal use 5 bytes for CO2 otherwise 7 Bytes Command list Requesting failure codes Command 1001 0000 90H Data none Response Reading failure codes Failure code The microprocessor returns a total of 10 bytes 5 integer values Each inte ger value rep...

Страница 31: ...required is transmitted Particularities during data communication Data request and response For the data communication between PC and microcontroller the following particularities must be observed The microprocessor stores an int or unsigned int value with the sequence Highbyte Lowbyte in the memory For the PC this sequence is reversed The microcontroller transmits these values in its format i e t...

Страница 32: ...rcuits an alarm message is issued to the connected alarm monitoring system The poten tial free contacts 1 changeover contact have been laid out for the follo wing circuits Alarm relay NOTE Switching structure For all failures reported by the device sensor circuit open deviation from the nominal value and door open for more than 10 minutes the alarm relay changes state Circuit Voltage External fusi...

Страница 33: ...alues for the operating voltage of the external circuits and of the fusing of the alarm system are given in the table above A 1 Connect the individual conductors 1 to 4 of the connecting cable as shown in the wiring diagram 2 Connect the connector 5 of the alarm system connecting cable to the interface 6 at the control box at the rear panel of the device Fig 15 Example of a connection scheme ...

Страница 34: ...regulations set forth by the operator to protect the cultures Water supply The water tray of the work space can hold up to 3 0 l of proces sed water When the running operation always keep a suf ficient quantity of processed water of the following quality available demineralized and distilled or autoclaved for sterilization or completely deionized and distilled or autoclaved for sterili zation When...

Страница 35: ... set amount of CO2 12 The device is ready for operation 13 Load work space with cultures NOTE Duration of the auto start routine When the device is cold and when the ambient temperature is low the auto start routine may take up to 10 hours Loading Max loading surface To ensure sufficient air circulation and even heating of the sam ples the loading surface within the work space should be used up to...

Страница 36: ...onal areas 2 displays that show numeric values for temperature and CO2 content 8 keys for selecting functions and for entering data 6 LEDs that show functions or operating states 3 Temperature display 4 Heating LED 5 Key for setting temperature nominal value 6 Key for increasing value 7 Key for reading failure codes stopping acoustic alarm 8 Key for activating auto start 9 LED for indicating door ...

Страница 37: ...Operating Instructions symphony 5 3 A 06 2009 7 Handling and control 7 Handling and control 7 Handling and control 37 Fig 16 Power switch Operating panel ...

Страница 38: ...hat the test routine is being run The temperature display shows a three digit number for the corre sponding assembly parameter set P 1 Operating and display board P 2 Measuring cell P 3 Main board P n Parameter number The CO2 display shows the software version device version 2 Test routine completed The temperature display shows the current temperature value the CO2 display shows the current CO2 v...

Страница 39: ...o reduce the nominal value Press the keys 3 Accept and store the nominal value Release both keys The temperature display shows the current actual value measured in the work space 7 5 Setting the CO2 nominal value 1 Indicate the nominal value Press the key The CO2 display shows the current nominal value CO2 nominal value 2 Enter the nominal value The nominal value can be increased or reduced in inc...

Страница 40: ...ration of the device The auto start rou tine should be run at least every three months on the occasion of cleaning and maintenance works Duration of auto start routine Running the routine usually takes 5 to 7 hours At low room temperatures and when the device is cold it may take up to 10 hours until the auto start routine has been completed If the glass door is opened or if the power supply of the...

Страница 41: ...em The device is equipped with a failure diagnostic system This system recognizes failures during the operation and allows the allocation of failure causes by numeric codes Failure recognition is displayed by an acoustic and a visual alarm at the operating panel The diagnostic system stores the last 10 failures in the sequence of their occurence The failure table can be requested and read If the c...

Страница 42: ...rs should be opened for some time if the nominal values are reduced 1 The audible alarm sounds 2 Silence the audible alarm Press any key The audible alarm is silenced 3 Read failure codes Keep the key depressed If no failure is detected each display shows three hyphens Indication of failure code If the system detects a failure the display that is assigned to the cor responding control circuit show...

Страница 43: ... Registered failure codes The temperature display shows the last 10 failure codes that had been registered The CO2 display shows the number of the indivi dual failure code within the table 5 Exit the failure code table Release the key The temperature and CO2 displays show the current actual values 6 Erase the failure code table Keep the depressed for 5 seconds The temperature and CO2 displays flas...

Страница 44: ...apsed or max adjustment value exceeded Repeat auto start 99 Device doors open Doors open for more than 10 minutes door switch Close device doors test door switch for correct function 100 Temperature below nominal value Actual value nominal value 1 C Contact Service 101 Temperature above nominal value Actual value nominal value 1 C Do not exceed ambient temperature limit 104 Temperature sensor faul...

Страница 45: ... Press the power switch NOTICE Thermal protection Reset When the cause of the failure e g excessive temperature in the operating room has been repaired the device is set to normal incubation operation after it has been turned on again If the cause of the failure cannot be repaired with simple measures e g by ventilating the room or by reducing the temperature in the operating room the thermal prot...

Страница 46: ...iners and all accessories from the work space 2 Pump water out off the water reservoir 3 Clean and decontaminate the work space and wipe device dry 8 2 Turn device off using the power switch 4 Unplug power connector and protect it against accidental reconnec tion 5 Close the CO2 supply system shut off valves 6 Disconnect gas pressure hoses from sleeve at the rear of the device 7 Until the device i...

Страница 47: ...ntaining solvents detergents with an alcohol content of more than 10 or powerful acids and bases Chloride containing disinfectants Chloride containing disinfectants may corrode stainless steel Use only disinfectants that do not affect stainless steel Do not use sodiumhypochlorite solutions such as Purex and Clorox These can cause corrosion and pitting of stainless steel Do not use steel wood pads ...

Страница 48: ...nection Check to see if the device is deenergized 1 CAUTION Health hazard The surfaces of the work space may be contaminated Contact with contaminated cleaning liquids may cause infections Disin fectants may contain harmful substances When cleaning and disinfecting always observe the safety instructions and hygiene regulations Wear safety gloves Wear safety glasses Wear mouth and respiratory syste...

Страница 49: ...e of the measuring cell Removing accessories and shelf system 1 Remove all shelves then remove the entire shelf system from the work space For removal and installation of the shelf system please refer to Section 5 3 2 If required remove the blower wheel and its cover from the baseplate of the measuring cell The wheel and the cover can be autoclaved Removing blower wheel and cover A 1 Fig 17 Remove...

Страница 50: ...the decontamination routine takes approx 25 hours 1 CAUTION Hot surface The surfaces of the exterior door handle work space particu larly the glass door armatures and the interior sheet of the outer door are heated during the decontamination routine During the routine run or immediately after completion of the run always wear safety gloves when tou ching these surfaces observe the warning indicato...

Страница 51: ...down to the preset temperature nominal value 2 C A Heating phase Remaining run time approx 25 hours The work space is heated to a temperature of 90 C while an elevated relative humidity is created The cur rent decontamination temperature is shown at the temperature display Decontamination phase Remaining run time approx 23 hours After the decontamination atmosphere has been created the decontamina...

Страница 52: ...s 3 Start the routine Keep the key depressed for 5 seconds The 90 C LED flashes Close the device doors The actual value is shown at the temperature display The remaining run time is shown at the CO2 display The door LED goes off 4 Complete the routine Keep the key depressed for 5 seconds Indication of running routine The display returns to the normal operating state incubation opera tion 9 3 3 Can...

Страница 53: ...heck If safety devices were removed or disabled for inspections the device must not be operated before the safety devices have been reinstalled and chekked for their correct function 10 2 Service intervals During normal operation the following service routine must be performed Service routines Weekly service Refill the work space water tray with fresh processed water 3 month service Run auto start...

Страница 54: ...r as the evaporation of water will result in a lower temperature reading Comparison measurement procedure 1 Turn device on using power switch 2 Set temperature nominal value and allow device to be heated This may take up to several hours A 3 Fig 19 Place measuring instrument 3 onto the center area of the work space Alternatively a temperature sensor may be posi tioned in this location Route the co...

Страница 55: ...he nominal value Press the key The preset value of 37 C is displayed 3 Enter the measured value destination value Press the keys or Press the keys Destination value e g 36 4 C 4 Accept the destination value Press the key The temperature display momentarily shows CAL Then the corrected actual value measured destination value 36 4 C is displayed 5 Cancel the calibration process Press any key The tem...

Страница 56: ...lass door or through the access port at the rear panel of the device The comparison measurement must be performed when the device is completely stable Comparison measurement procedure 1 Turn device on using power switch 2 Set CO2 nominal value and allow device to heat up completely and to create humidity This pro cess may take several hours A 3 Fig 20 Route the measuring instrument either through ...

Страница 57: ...he nominal value Press the key The set nominal value of 5 is displayed 3 Enter the measured value destination value Press the keys or Press the keys Destination value e g 5 6 4 Accept the destination value Press the key The CO2 display momentarily shows CAL then the corrected actual value measured destination value 5 6 is displayed 5 Cancel the calibration process Press any key The temperature dis...

Страница 58: ...carefully all the way to the stop 7 Install retainer 1 8 Connect gas hose to sterile filter sleeve and secure it using hose clamp Check to see if the gas hose is securely seated on the sleeve 10 8 Replacing the device fuses Fig 22 The two identical device fuses 4 are installed in the fuse compartment 1 next to the power plug receptacle of the device Time delay fuses 6 3 A 5x20 mm 1 The fuse holder...

Страница 59: ...door is loca ted in the retaining slot No tools are required to replace the seal 1 Fig 23 Pull magnetic seal 3 out of the guide slot 1 2 Position new seal at a corner 2 and press seal retaining rail 4 into slot 3 Make sure that the retaining rail taper is positio ned correctly in the slot 1 and that the seal is flush with the door frame Fig 23 Door seal replacement ...

Страница 60: ... the Technical Support for parts and accessories please have the following information available Model Number Serial Number Date Purchased NOTE Repairs Use only original parts that have been tested and approved by VWR The use of other parts presents potential hazards and will make the warranty void Additional parts For additional parts please contact VWR Service Organisation ...

Страница 61: ...mperature range C 18 33 Temperature control range C RT 3 55 Temperature deviation at 37 C time C 0 1 Temperature deviation spatial DIN 12880 Part 2 at 37 C C 0 6 Duration of the auto start routine to 37 C ambient temperature 20 C h 5 10 Temperature recovery time at 37 C door open 30 seconds to 98 of initial value min 10 Heat transfer to environment at 37 C during 90 C decontamination kWh h kWh h 0...

Страница 62: ... 3 Rated voltage V V V 1 N PE 230 V AC 1 N PE 120 V AC 1 N PE 100 V AC Rated frequency Hz 50 60 Interference suppression DIN VDE 0875 Interference level N Type of protection DIN 40 050 IP 20 Protection class l Overvoltage category IEC 1010 EN 61010 ll Pollution severity IEC 1010 EN 61010 2 Rated current A 5 3 120 V AC On site fusing Fuse Circuit breaker T 16 A G 16 Rated input kW 0 64 120 VAC EMC ...

Страница 63: ...Operating Instructions symphony 5 3 A 06 2009 12 Technical data 12 Technical data 12 Technical data 63 Average gas consumption Fig 24 CO2 consumption ...

Страница 64: ...be decon taminated The device components must be cleaned thoroughly after the cleaning they must be disinfected or sterilized as requi red by the application Discarded devices or device components must be provided with an appropriate certificate showing the decontamina tion measures performed All device components can be discarded properly after they have been decontaminated properly NOTE Recyclin...

Страница 65: ...eet painted Door inner panel Galvanized steel sheet painted Operating panel and indicator foil Polyethylene Magnetic door seal Magnetic core sheathed with EMPP Heating Silicone sheathed resistance type wires Interior containers installed components and shelves Stainless steel 1 4301 Plug for pipe channel Silicone Pressure compensation opening insert POM with brass sinter filter Glas screen Soda si...

Страница 66: ... 2009 66 14 Device log Record following informations here nameplate information work carried out maintenance work and repairs Part number Serial number Service number Location Operator s note Date Signature Device type Work carried out Notes ...

Страница 67: ...Operating Instructions symphony 5 3 A 06 2009 67 15 Certificate of decontamination ...

Страница 68: ...www vwrsp com ...

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