VWR avantor 700-1472 Скачать руководство пользователя страница 15

10.  Troubleshooting

Problem

Causes

Resolutions

No Migration

Power supply not 
operating or not 
correctly connected, or 
gel inserted backwards

Check the proper operation of the 
power supply and proper connection 
of the power leads to + and - outputs.  
Check that the gel is properly oriented 
(DNA will migrate t electrode)

Abnormal 
Electrophoresis 
speed.

Incorrect voltage 
setting, incorrectly 
prepared buffer.

Check power supply output, check 
that buffer is the proper type and 
appropriate concentration.

Abnormal heating of 
buffer or gels

Buffer concentration 
too high, voltage too 
high, or buffer has 
been reused too many 
times.

Use 0.5X TAE/TBE buffer or reduce 
running voltage.  If buffer has been 
used in previous runs, replace with 
freshly prepared buffer.

11.  Equipment disposal  

This equipment is marked with the crossed out wheeled bin 
symbol to indicate that this equipment must not be 
disposed of with unsorted waste.
Instead it's your responsibility to correctly dispose of your 
equipment at lifecycle -end by handling it over to an 
authorized facility for separate collection and recycling. It's 
also your responsibility to decontaminate the equipment in 
case of biological, chemical and/or radiological 
contamination, so as to protect from health hazards the 
persons involved in the disposal and recycling of the 
equipment.
For more information about where you can drop off your 
waste of equipment, please contact your local dealer from 
whom you originally purchased this equipment.
By doing so, you will help to conserve natural and 
environmental resources and you will ensure that your 
equipment is recycled in a manner that protects human 
health.  Thank you  

14

vwr.com |  Instruction manual VWR® Real Time Electrophoresis Tank 

Содержание avantor 700-1472

Страница 1: ...VWR Real Time Electrophoresis Tank System North American Catalog Number 76359 754 European Catalog Number 700 1472 This product is for research use only Version 1 Issued 08 2021 Instruction manual...

Страница 2: ...earchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven 3216385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leicestershire LE17 4XN uk vwr com United Stat...

Страница 3: ...re any parts missing damaged or incorrect please contact your distributor or VWR s Customer Service Department Table of Contents 1 Introduction 2 1 1 Included Component List 3 1 2 Diagrams of Included...

Страница 4: ...r casting the agarose gels The blue LED transilluminator and viewing filter are compatible with green fluorescent stains such as VWR s EZ Vision Products SYBR Green and other similar stains that fluor...

Страница 5: ...Filter and Power Leads Gel Running Tank Blue LED Transilluminator 12V Power Supply Smart phone imaging enclosure with photo filter 1 2 Diagrams of Included Components 4 vwr com Instruction manual VWR...

Страница 6: ...ded Components Gel Casting Set Double Sided Comb 12 22 teeth Long Gel Tray 10 5 x 10cm Short Gel Trays 10 5 x 6cm Gel Casting Divider Gel Casting Stand 5 vwr com Instruction manual VWR Real Time Elect...

Страница 7: ...22 5 6mm 1mm 12 Gel trays 10 5cm x 11cm and 10 5cm 6cm 2 Installation Place the electrophoresis tank on a smooth level surface Confirm that the power supply to be used is suitable for the voltage and...

Страница 8: ...supply cords from the safety lid and do not remove the safety lid when the attached power supply is running Attempting to do so can cause damage and can also create a shock hazard Use only appropriat...

Страница 9: ...the gel tray is not level the thickness of the gel will not be uniform and DNA migration may be uneven Insert the comb s into the gel casting stand as per image on the right Multiple combs can be ins...

Страница 10: ...tes hold the two sides of comb and gently lift it out of the gel The wells should be straight and undamaged Carefully remove the gel tray with the gel from the stand and place it into the migration ta...

Страница 11: ...with the gel tray onto the gel bed inside the gel tank Carefully pour an appropriate buffer solution to a level of about 2 mm above the surface of the gel Typically 200ml of buffer is required It is...

Страница 12: ...ttom of the wells Carefully lower the safety cover onto the gel tank The orange viewing filter should fully contact the surface of the buffer so there are no bubbles visible If the buffer level is too...

Страница 13: ...s The LED illuminator has a built in timer so the light will automatically switch off after 5 minutes Connect the positive red and negative black power leads from the safety cover to the and outputs o...

Страница 14: ...ure 9 Maintenance Always disconnect the 12V power supply when cleaning the transilluminator When cleaning the surfaces of the instrument and LED transilluminator use a damp cloth with mild detergent i...

Страница 15: ...ent disposal This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed of with unsorted waste Instead it s your responsibility to correctly...

Страница 16: ...plication or from ordinary wear and tear If the required maintenance and inspection services are not performed according to the manuals and any local regulations such warranty turns invalid except to...

Страница 17: ...2 Email sales ie vwr com Italy VWR International S r l Via San Giusto 85 20153 Milano MI Tel 39 02 3320311 02 487791 Email info it vwr com Korea VWR International 2F ACE Gwanggyo Tower I Daehak 4ro 17...

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