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Instruction manual | 

vwr.com

 

3

Table of contents

1. Warning 

4

2. 

Safety Information 

4

3. 

Package Contents 

5

 

3.1  IT406 FL2 BG 

5

 

3.2  IT406 FL4 

6

 

3.3  IT406 FL4D 

7

4. Unpacking 

8

5. 

Intended use 

8

6. 

Symbols and conventions 

8

7. 

Instrument description 

9

 

7.1 IT406 FL2 BG 

9

 

7.2 IT406 FL4 

10

 

7.3 IT406 FL4D 

11

8. 

Installation  

13

 

8.1 Installing the objectives 

13

 

8.2 Installing stage extension or mechanical stage 

13

 

8.3 Installing the stage insert 

14

 

8.4 Installing the eyepieces 

14

 

8.5 Installing the condenser  

14

 

8.6 Installing the LED housing  

15

 

8.7 Installing color filters 

15

 

8.8 Installing the PC screen (IT406 FL4D) 

16

 

8.9 Cable connection (IT406 FL4D) 

16

 

8.10 Connecting the power supply 

17

9. 

Brightfield observation (transmitted light) 

18

10. 

Use of the microscope in brightfield (transmitted light)  19

 

10.1 Turning on the microscope 

19

 

10.2 Adjusting the light intensity 

19

 

10.3 Adjusting the coarse focus tension 

19

 

10.4 Diopter adjustment 

19

 

10.5 Adjusting interpupillary distance 

20

 

10.6 Use of eyeshields 

20

 

10.7 Selecting the light path 

20

 

10.8 Stage and stage inserts 

21

 

10.8.1 Installing stage inserts 

21

 

10.9 Aperture diaphragm 

22

 

10.10 Using color filters 

22

11. 

Use of the microscope in Phase Contrast 

 

(transmitted light) 

23

 

11.1 Installing the phase contrast slider 

23

 

11.2 Phase contrast slider 

23

 

11.3 Centering the phase ring 

23

12. 

Fluorescence observation (reflected light) 

25

13. 

Use of the microscope in fluorescence (reflected light)  26

 

13.1 Using the microscope 

26

 

13.2 Switching fluorescence filter cubes 

26

 

13.2.1 Available fluorescence filter cubes 

26

 

13.3 Installing fluorescence filter 

27

 

13.4 Use of the Anti-glow cap 

28

 

13.5 Use of the UV shield 

28

14. 

Simultaneous observation in Phase 

 

Co Fluorescence 

29

15. Microphotography 

30

 

15.1 Use of C-mount cameras 

30

 

15.2 Use of Reflex cameras 

30

16. Troubleshooting 

31

17. 

Repair and maintenance 

33

18. 

Technical service 

33

19. Warranty 

33

20. 

Compliance with local laws and regulations 

33

 Disposal 

34

Содержание 630-3254

Страница 1: ...Instruction manual VWR Microscope VisiScope Series 400 VERSION 02 ISSUED 03 2021 EU cat no 630 3254 630 3256 630 3255...

Страница 2: ...CTURER Europe VWR International bv Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven 32 16 385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leices...

Страница 3: ...the coarse focus tension 19 10 4 Diopter adjustment 19 10 5 Adjusting interpupillary distance 20 10 6 Use of eyeshields 20 10 7 Selecting the light path 20 10 8 Stage and stage inserts 21 10 8 1 Insta...

Страница 4: ...responsibility deriving from incorrect instrument use that does not comply with this manual 2 Safety Information Avoiding Electrical Shock Before plugging in the power supply make sure that the suppl...

Страница 5: ...hase contrast slider 7 Glass insert for stage 8 Metal insert for stage 9 Objectives 10 Eyepieces 11 Stage inserts 12 Centering telescope 13 Green filter IF550 14 Lens cleaning tissue 15 UV shield 16 D...

Страница 6: ...ondenser 3 LED illuminator 4 Objectives 5 Eyepieces 6 Anti glow cap 7 Glass insert for stage 8 Metal insert for stage 9 Mechanical stage 10 UV shield 11 Stage inserts 12 Lens cleaning tissue 13 Dust c...

Страница 7: ...bjectives 6 Eyepieces 7 Glass insert for stage 8 Metal insert for stage 9 Mechanical stage 10 Stage inserts 11 Camera 12 C mount 13 Mouse 14 Keyboard 15 Monitor 16 UV shield 17 Power supply power cord...

Страница 8: ...ds one around the neck and one around the base lift the microscope from the container and put it on a stable desk 5 Intended use For research and teaching use only Not intended for any animal or human...

Страница 9: ...MENT RING EYEPIECE PHASE CONTRAST SLIDER LED HOUSING FILTER HOLDER COARSE FOCUS KNOB FINE FOCUS KNOB OBJECTIVE STAGE MICROSCOPE BODY LIGHT PATH SELECTOR LEVER STAGE PLATE CONDENSER PHOTO TV PORT FLUOR...

Страница 10: ...EPIECE LED HOUSING FILTER HOLDER COARSE FOCUS KNOB FINE FOCUS KNOB OBJECTIVE STAGE MICROSCOPE BODY LIGHT PATH SELECTOR LEVER STAGE PLATE CONDENSER PHOTO TV PORT FLUORESCENCE CUBE LEVER TRANSMITTED LIG...

Страница 11: ...G EYEPIECE FILTER HOLDER COARSE FOCUS KNOB FINE FOCUS KNOB OBJECTIVE STAGE MICROSCOPE BODY LIGHT PATH SELECTOR LEVER STAGE PLATE CONDENSER FLUORESCENCE CUBE LEVER TRANSMITTED LIGHT ADJUSTMENT KNOB REF...

Страница 12: ...al 12 IT406 FL4D back side MONITOR POWER SUPPLY CABLE HDMI PLUG PC STICK HDMI CABLE TO MONITOR USB CABLE TO CAMERA MONITOR POWER SUPPLY CONNECTOR PC STICK POWER SUPPLY CABLE LED POWER SUPPLY CABLE USB...

Страница 13: ...re very sensitive to dust To prevent dust and contamination from entering the microscope cover all the unused holes with dust caps 3 Fig 3 When operating use the low power objective 10X to search and...

Страница 14: ...pieces into the tubes of the optical head Fig 6 8 5 Installing the condenser 1 Insert the condenser illumination unit into the bracket Fig 7 2 Turn the condenser illumination unit clockwise about 90 w...

Страница 15: ...luminator No operation is therefore necessary Fig 10 8 7 Installing color filters Remove the filter holder then install the color filter you need Fig 11 12 1 Mount the color filter flat as shown in 1...

Страница 16: ...eel 3 to lock the monitor Fig 14 8 9 Cable connection IT406 FL4D 1 Connect the USB mouse keyboard receiver in the slot A of the PC stick 2 Plug the USB3 0 cable in the slot B of the PC stick 3 Plug th...

Страница 17: ...8 8 10 Connecting the power supply 1 Turn the main switch to O OFF before connecting the power supply 2 Insert the jack of the power supply into the power socket of the microscope Fig 19 3 Plug the po...

Страница 18: ...pecimen Begin observation Adjust aperture diaphragm Insert in the light path the desired objective and focus the specimen Adjust interpupillary distance Adjust diopter Adjust brightness Main switch Ob...

Страница 19: ...or the specimen defocuses while adjusting the fine focus knob 5 the coarse focus knob is too loose Turning the tension adjustment collar 4 in clockwise direction tightens the coarse focus tension 3 Ro...

Страница 20: ...lasses Fold rubber eyeshields with both hands Folded eyeshields avoid scratching the lenses of eyeglasses Fig 25 Use without eyeglasses Raise eye shields and observe at the microscope placing eyes to...

Страница 21: ...hand or by turning the knobs 1 of the mechanical stage Fig 28 When switching objectives take care not to touch the holder plates with the objectives as their weight may damage the front lens 10 8 1 In...

Страница 22: ...e With low contrast specimens move the Aperture Diaphragm lever AS 1 to set the numerical aperture to about 70 80 of the objective s N A Fig 31 If necessary remove one eyepiece and looking into empty...

Страница 23: ...via the two side bolts see chapter 10 3 4 The 4x 10x position 2 must be used with 4x and 10x phase contrast objectives the 20x 40x position 3 with the 20x and 40x and the SL position 4 is used for br...

Страница 24: ...in the phase contrast slider using a screwdriver until the bright ring and the dark ring match Fig 38 4 The 4x and the 10X phase contrast objectives use the same ring on the phase contrast slider The...

Страница 25: ...e microscope in fluorescence reflected light 13 1 Using the microscope Move the main switch in the position I to turn on the microscope then rotate the adjustment knob 1 Fig 40 to adjust the fluoresce...

Страница 26: ...s provided with the fluorescence cube fix the new cube on the slider Fig 43 1 2 FIGURE 42 FIGURE 43 Filter name Excitation filter Dichroic mirror Barrier filter Applications VisiCube IT_B 325 375 nm 4...

Страница 27: ...h rapid decay must first be observed in Fluorescence and then in Phase Contrast The combined observation allows you to easily identify some areas of the sample that emit fluorescence 1 Turn on the mic...

Страница 28: ...apter 5 Connect the Reflex camera 4 to the T2 ring just installed Fig 49 6 Mount the other end of the relay tube 1 into the empty hole of the trinocular port then tighten the clamping screw Fig 47 T2...

Страница 29: ...th the objective phase ring Adjust the condenser ring to match the objective phase ring The light ring and or the phase contrast ring is not centered Adjust the bolts to center them The objective used...

Страница 30: ...n them For the best results use the VWR cleaning kit If you need to send the microscope to manufacturer for maintenance please use the original packaging 18 Technical service Web Resource Visit the VW...

Страница 31: ...It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposa...

Страница 32: ...14 85 00 international info fr vwr com 0 18 TTC min prix appel POLAND VWR International Sp z o o Limbowa 5 80 175 Gdansk Tel 48 58 32 38 200 info pl vwr com PORTUGAL VWR International Material de Labo...

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