| Instruction manual
22
2
Multi well plates can be directly inserted in the mechanical
stage. (Fig. 30)
10.9 Aperture diaphragm
The Numerical Aperture (N.A.) value of the aperture diaphragm
affects the image contrast. Increasing or reducing this value
one can vary resolution, contrast and depth of focus of
the image.
With low contrast specimens move the Aperture Diaphragm
lever (AS) (1) to set the numerical aperture to about 70%-80%
of the objective’s N.A. (Fig. 31)
If necessary, remove one eyepiece and, looking into empty
sleeve, adjust the condenser’s ring in order to obtain an image
like the one in fig. 32.
10.10 Using color filters
Selecting the appropriate color filter according your need.
(Fig. 33)
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
1
FIGURE 30
FIGURE 31
FIGURE 32
FIGURE 33
Filter
Use
Green (IF550)
Phase contrast microscopy
