5
Configure Spectrometer data-collection dialog box
For most experiments, the default settings work well.
There are four parameters listed in the dialog box.
l
Sample Time:
This is similar to the shutter speed of a camera. Logger
Pro
automatically selects the proper sample time during calibration.
Note:
For
emission studies, you may need to change the sample time manually.
l
Wavelength Smoothing:
This is the number of adjacent readings on either
side of a given value that is used to calculate an average value.
Note:
Be
careful adjusting this parameter as it may shift your wavelength values
slightly.
l
Samples to Average:
This is the number of readings taken at a given
wavelength to calculate an average reading.
l
Wavelength Range:
The range is determined by the type of
Spectrophotometer in use.
By clicking on the picture of the Spectrophotometer in this dialog box, you will
gain access to four options: calibrate, configure data collection, go to the
support website, and current connection. Click on an item to select it.
can display and analyze these graphs without a spectrometer connected to your
computer. Follow these steps to view one of these graphs.
1. Choose Open from the File menu.
2. Open the Sample Data folder.
3. Inside the Sample Data folder, open the Physics folder.
4. Inside the Physics folder, open the Gas Discharge Spectra. Open the desired
file.
You can use the mercury emissions graph to test fluorescent lighting for the
presence of mercury.
Measure Fluorescence with Logger
Pro
You may use your Spectrophotometer to measure the fluorescence spectrum of
an aqueous sample, such as chlorophyll, riboflavin, and fluorescein.
Fluorescence is the emission of light by a compound after it has absorbed a
particular wavelength of light. Under most circumstances, the emission of light
will occur at a longer wavelength than the light used to excite it. Go Direct
SpectroVis Plus has two excitation wavelengths, one at 405 nm and one at
500 nm.
There are three general types of data collection that measure fluorescence—
fluorescence
vs.
wavelength, which produces a spectrum, fluorescence
vs.
concentration, and fluorescence
vs.
time for kinetics experiments. Once the units
have been changed to Fluorescence from the Experiment menu, follow the
instructions in the Collect Data with Logger
Pro
section of this user manual to
collect these types of data.
Note:
You may need to change the sample time to
get accurate peak intensities in fluorescence mode. To adjust the sample time
choose Set Up Sensors ► Spectrophotometer: 1 from the Experiment menu. Set
the Sample Time to a suitable value. This value can be adjusted during data
collection.
Change the Settings in Logger
Pro
Spectrophotometer Dialog Box
The Spectrophotometer dialog box lists all the settings for the device. To
display this box, choose Set Up Sensors ► Spectrophotometer from the
Experiment menu.
