V&P Scientific, Inc.
2
Technical Note 115A
SETUP FOR ASPIRATION MANIFOLD ON JIG
Attaching the Manifold
1.
Loosen Manifold Hold-Down Screws (3) using the 1/16 Allen wrench and remove the
Manifold Hold Down Bars (4) (Figure 1).
2.
Place the shoulder bolt heads (2) of the Manifold into the Jig slot (1) and slide down
into the slots until shoulder bolts hit the bottom of the slots.
3.
Place the Manifold Hold-Down Bars (4) on top of the Manifold and tighten the
Manifold Hold-Down Screws (3) to secure the Manifold in the Jig (Figure 2a).
Setting the X and Y Positions
1.
Slide a microplate into the Microplate Platform (13). Use the Allen wrench provided to
adjust the X and Y Manifold position so it is in the center of the wells of the microplate
(Figure 2a and 2b).
2.
When adjusting the X Position always loosen the X Positioning Screws first in the
direction the manifold is to be moved before tightening the opposing X Positioning
Screws. Failure to do this can pull the Shoulder Bolt (2) out of the Manifold and strip
the threads.
Figure 2: Securing Manifold in Jig (a). Adjusting X (a) and Y (b) Positions.
3.
For more precise positioning use one of the Registration Plates VP 903R-96, VP 903R-
384 or VP 903R-1536 (Figure 3). Place the Registration Plate into the Microplate
Platform (13) where the microplate would go and push against the back wall. Pull the
Z Arm Lever (8) down until the Manifold tubes are just over the Registration Plate.
Tighten the Z Arm Nylon Press Screw (9) to hold the Microplate Platform (13) in
position (Figure 4). Line up the white 1mm corner spots of the Registration Plate with
the corner tubes on the Manifold. This is done by using the provided 0.5 Allen wrench
to adjust the X and Y Position Screws (5 and 6) as shown in Figure 2.
7
8
6
2a
2b
3
5
