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Aqua

Flash U

ser’s Manual 

18 

Appendix C:   Calibrating the 

Aqua

Flash 

 

 
The 

Aqua

Flash can be user calibrated.  This action will overwrite the factory calibration which 

can only be restored using 

Aqua

Flash Calibration Solution P/N 8600-225 purchased from 

Turner Designs.   
 

NOTE:  Do not overwrite the factory calibration unless you are sure you have the proper 
standards for calibrating the AquaFlash. 
 

Calibration Procedure 
 
The materials required for calibrating the 

Aqua

Flash are: 

 

-

 

Two glass cuvettes

 

-

 

Standard that has a known chlorophyll concentration value; this can either be live 
algae or a secondary standard, such as a dye solution.

 

-

 

Deionized water

 

-

 

Kim wipes

 

-

 

Aqua

Flash instrument

 

 

1.  Turn the 

Aqua

Flash on and wait until the warm up has completed. 

2.  Press the CAL button. 
3.  Press the ENTER button. 
4.  Fill a cuvette ¾ full with deionized water. 
5.  Insert the cuvette into the 

Aqua

Flash and press ENTER. 

6.  When measurement has completed, remove the cuvette from the instrument. 
7.  Fill a second cuvette ¾ full with the standard solution. 
8.  Insert the cuvette into the 

Aqua

Flash and press ENTER. 

9.  When measurement has completed, remove the cuvette from the instrument. 
10. Press the CAL VAL button and adjust using the 



 arrow keys to set the value 

displayed to match the standard solution

’s known chlorophyll (

µ

g/L) concentration. 

 

-

 

If the message “Standard<Blank Recalibrate<ENT>” is displayed, it means the standard 
solution used didn’t generate a response higher than the blank.  Either the standard being 
used isn’t appropriate for this application or a standard with a higher concentration is 
required to proceed with calibration. 
 

-

 

If the message “RE-CAL USING HIGHER CONC.” is displayed, it means the standard 
solution’s response was slightly higher than the blank’s response but not high enough and 
a standard with a higher concentration is required to proceed with calibration. 
 

-

 

If the message “RE-CAL USING LOWER CONC.” is displayed, it means the standard 
solution’s response was too high and a standard with a lower concentration is required to 
proceed with calibration.  You can either dilute the standard by half and re-calibrate or 
obtain a new standard.   

Содержание AquaFlash

Страница 1: ...P N 998 8601 Revision A January 14 2016 TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 ...

Страница 2: ...ication and Function 6 2 2 Instrument Power Up 7 2 3 Calibration Check 8 2 4 Measuring a Sample 9 2 5 Data Displayed 10 2 6 Downloading Data 10 2 7 Installing the Interface Integration Adaptor Cable 11 3 Sample Analysis Guidelines 12 4 Measurement Parameters 4 1 Chlorophyll 13 4 2 Photosynthetic Efficiency 13 5 Warranty 5 1 Terms 14 5 2 Warranty Service 14 5 3 Out of Warranty Service 14 Appendixes...

Страница 3: ...end of life product proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at...

Страница 4: ...c phytoplankton fluorescence characteristics which are used to directly estimate the photosynthetic efficiency of algae or plant material There are two measurement modes single and multiple turnover The AquaFlash is a multiple turnover PAM fluorometer in that one saturating pulse of light is delivered to the sample to quickly reach the maximum fluorescence value with the interest of looking at how...

Страница 5: ...N 2300 115 1 5 General Information Precautions and Cleaning The plastic battery tab at the back of the AquaFlash must be removed before use Grasp it firmly and pull gently to remove it to activate the batteries that power the AquaFlash Use caution around solvents they may degrade the plastic case of the AquaFlash If a sample is accidentally spilled inside the sample compartment invert the AquaFlas...

Страница 6: ... the sample analysis procedure detailed in section 2 4 ON OFF Turns the instrument ON or OFF ENTER Allows users to proceed to the next screen menu DATA When the DATA button is pressed users can choose between either sending clearing data or setting date time Use the keys to choose between the two options a marker will indicate the selection made If SEND CLEAR DATA is selected press ENTER and a sub...

Страница 7: ...ing whether there is drift in the AquaFlash s calibration SSS is available for purchase as accessory P N 8000 951 2 2 Instrument Power Up Press the ON OFF button to power on the AquaFlash After a 5 second warm up the AquaFlash is ready for operation Pressing the ON OFF button again will turn the unit off or if left idle on the HOME screen for 3 minutes the unit will automatically turn off to save ...

Страница 8: ...n reading the Adjustable Solid Secondary Standard SSS 5 Press the READ button a If the CHL value displayed is lower than the calibration value determined in step 2 adjust the SSS to give you a larger response by rotating the silver hex screw located at the top of the SSS counterclockwise and re read the SSS b If the CHL value displayed is higher than the calibration value determined in step 2 adju...

Страница 9: ...o your AquaFlash and close the lid 5 Press READ again and the measurement will begin 6 The measurement is complete remove the cuvette and discard the sample 7 Results will be displayed as shown below 8 Press the down arrow key to view Fo minimum Fm maximum and Blank raw fluorescence values 9 When finished viewing data or if ready to run the next sample press ESC to get to the Home screen and repea...

Страница 10: ...imit of the instrument 2 6 Downloading Data If your computer does not have a serial port available you will need to install the Interface Integration Adaptor Cable see section 2 7 before proceeding Once installed plug the Data Download Cable into the Adaptor Cable and continue with Downloading Data instructions If your computer has a serial port plug the Data Download Cable into the serial port an...

Страница 11: ...e to view data before clearing data from the AquaFlash 2 7 Installing the Interface Integration Adaptor Cable This cable allows you to convert from RS232 to USB You will need the USB drive and the Interface Integration Adaptor Cable both included in the AquaFlash package to complete the installation 1 Plug the USB drive into your computer 2 Double click the InstallParallaxUSBDriversv2 08 02 exe fi...

Страница 12: ...tamination between subsequent samples will skew results Three rinses with the sample intended for measurement will help flush out any residual and decrease the chance for cross contamination Do not use a cuvette cap as it may cause the cuvette to not seat properly in the sample compartment When handling the cuvette hold it near the top Any bubbles in the sample will affect the readings Take care n...

Страница 13: ...e abundance estimates 4 2 Photosynthetic Efficiency Yield The AquaFlash uses two measuring LEDs to estimate photosynthetic efficiency The first LED monitoring is used to excite the sample with very low light intensity so as not to induce a change in chlorophyll reaction centers While continuously monitoring the sample using the monitoring LED the second LED saturating blasts the sample with a high...

Страница 14: ...t turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Authorization number RMA Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be r...

Страница 15: ...AquaFlash User s Manual 15 ...

Страница 16: ...hargeable batteries Auto Power Off After 3 minutes of inactivity on the HOME screen only Light Source LED Detector Photodiode LCD Display 2 x 16 characters Resolution 12 bits Case Meets IP 67 Standard dustproof and waterproof Temperature 41 104 F 5 40 C Internal Memory 1000 records Data Output Format ASCII Weight 0 87 lbs 0 4kg Size 1 75 x 3 5 x 7 25 4 45cm x 8 9cm x 18 4cm ...

Страница 17: ...wnload data to your PC and clear the data log When memory is full the instrument will display Memory Full When READ is pressed you will not be allowed to analyze samples until data have been downloaded and cleared from memory Battery Warning Messages Battery power is checked whenever users turn the instrument on using the ON OFF button After the 5 second countdown If the battery power is 20 the fo...

Страница 18: ...Fill a second cuvette full with the standard solution 8 Insert the cuvette into the AquaFlash and press ENTER 9 When measurement has completed remove the cuvette from the instrument 10 Press the CAL VAL button and adjust using the arrow keys to set the value displayed to match the standard solution s known chlorophyll µg L concentration If the message Standard Blank Recalibrate ENT is displayed it...

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