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Turner BioSystems GloRunner, Руководство по эксплуатации

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www.turnerbiosystems.com

4

I. 

Introduction

Description

The GloRunner

 is an easy-to-use, sensitive luminometer with a broad

dynamic range designed to read glow luminescent reactions in 96-well
plates.  The instrument’s small size and affordable price make it an
ideal laboratory instrument.  This manual is designed to guide you
through the installation, setup, calibration, and operation of your
instrument.

Inspection

Upon receiving your luminometer, please inspect it carefully and make
sure all accessories are present (refer to the packing list shipped with
the instrument).

Standard accessories:

v

 

Power Cord

v

 

9-pin Serial Cable

v

 

Operating Manual

v

 

Quick Start Guide

v

 

Software

 General Precautions

v

 

This instrument is intended for indoor use only.

v

 

A clear, transparent adhesive film should be placed over the 96-
well microplate to prevent spills or cross-contamination between
wells.

1

v

 

Wipe up spills immediately. See Appendix C for cleaning
instructions.

v

 

The luminometer contains sensitive optical components and
precision-aligned mechanical assemblies. Avoid rough handling.

                                                       

1

 This adhesive film may be purchased at Rainin, catalog no. 96-SP-100

  or any similar manufacturer.

Содержание GloRunner

Страница 1: ... GloRunner Microplate Luminometer Operating Manual Dated 09 18 2006 Version 1 2 Part Number 998 9005 645 N Mary Avenue Ÿ Sunnyvale CA 94085 Phone 408 636 2400 Ÿ Toll Free 888 636 2401 Fax 408 737 7919 Ÿ www turnerbiosystems com ...

Страница 2: ...nometer DXL Software 7 Overview 7 Software Operation 9 Communications Settings Procedure 11 GloRunner User Interface Features 13 Setting the Options 14 V Reading Samples 16 Starting a Run 16 VI Calibration of the GloRunner 17 Procedure 17 VII Relative Light Units and Common Questions 19 Overview 19 FAQs 19 APPENDIX A Installing the Software 21 Overview 21 Procedure 21 APPENDIX B LED Messages and F...

Страница 3: ... Warranty Service 24 Maintenance 24 Warranty 24 Obtaining Service 25 Warranty Service 25 Out of Warranty Service 26 Address for Shipment Error Bookmark not defined APPENDIX D Specifications 27 Specifications for the GloRunner Microplate Luminometer 27 ...

Страница 4: ... all accessories are present refer to the packing list shipped with the instrument Standard accessories v Power Cord v 9 pin Serial Cable v Operating Manual v Quick Start Guide v Software General Precautions v This instrument is intended for indoor use only v A clear transparent adhesive film should be placed over the 96 well microplate to prevent spills or cross contamination between wells 1 v Wi...

Страница 5: ...tial component failure within the instrument See Appendix B for a detailed description of what the lights represent v Sample Tray Sample plates are placed on the tray for reading The plate format for this version is 96 well No other well format can be read v Drawer The drawer opens to receive sample plate v Power Switch The ON OFF switch is located on the back panel of the instrument v Power Cord ...

Страница 6: ...iosystems com 6 Power Cord Connection Power Switch RS 232 Serial Port Connection Figure 3 LED Status Lights Sample Tray Figure 1 9 pin Male Connector To GloRunner Figure 2 9 pin Female Connector To Computer Drawer ...

Страница 7: ...male 9 pin connector attaches to your computer See Figure 2 5 Turn on the power switch rear of unit Note The sample tray opens and closes via the software user interface To open the sample tray please proceed to the next section for further instructions IV GloRunner Microplate Luminometer DXL Software Overview The GloRunner Microplate Luminometer DXL software is very easy to use This direct to Exc...

Страница 8: ...If you elect to review the tutorial you will see the following tutorial window The tutorial can be printed by clicking the Print button When you are done reviewing the tutorial click the Close button to close the window Figure 5 ...

Страница 9: ... first using the GloRunner The second option is to use an existing workbook This feature allows you to add data to an existing workbook This should be selected if you would like to keep a running log of data from multiple assays If you are using the luminometer for the first time select the Create a new workbook option Once this is selected you will be prompted to select a data storage template fo...

Страница 10: ...s Figure 8 The top window is the GloRunner dialog box The bottom window is the active Excel spreadsheet in which data from the run will appear The dialog box is used for starting runs changing or selecting options toggling the tray position and establishing communication between the GloRunner and the computer If communication has not already been established between the GloRunner and the computer ...

Страница 11: ...unner dialog box pull down menu and the screen in Figure 9 will be displayed Figure 9 2 Select the proper COM port and click on OK The COM port selection depends upon the computer that is connected to the instrument If you are unsure of which COM port to select select COM 1 and proceed to the next step Figure 10 ...

Страница 12: ...the one displayed in Figure 12 go back to step 2 and select the next COM port For most computers the COM port selection will need to be either COM 1 or COM 2 If no error message appears and the tray door opens this is the correct COM port Please note the COM port selection for future reference Figure 11 Figure 12 ...

Страница 13: ...following message is used to indicate that the GloRunner is getting ready to take readings Figure 14 The GloRunner tray detection mechanism is helpful in two ways v This mechanism prevents plates being unintentionally left in the GloRunner If you have not selected the option to have your plate stored inside the instrument after the run the plate will be automatically ejected This will help prevent...

Страница 14: ...r multiple runs You can select up to 999 runs of the same plate with up to a 999 second delay between the end of one run and the start of the next run 4 The GloRunner will automatically eject the microwell plate at the end of the run If you prefer to temporarily store the plate within the instrument after the run select the Delay before microplate ejection option The plate can be stored inside the...

Страница 15: ...n in the top left corner of the grid Alternatively individual rows and columns can be selected or de selected by pressing the letter or number at the head of the row or column Select or de select individual wells by clicking on that well 7 Once you have made your selections click the OK button 8 The Excel Spreadsheet also contains the reading parameters as seen in Figure 18 Figure 18 ...

Страница 16: ...closed click the Tray In Out button on the GloRunner Dialog Box 4 Insert the plate in the sample tray so that well A1 is positioned to the right rear of the sample tray 5 Click on the Start button in the software to close the sample tray and start the instrument 6 The GloRunner will read the whole plate in approximately 30 seconds if the measurement duration is set at 1 second Once the run is comp...

Страница 17: ... determined that detector normalization is required choose Utilities on the pull down menu Next select Calibrate Using Light Plate You will see the following pop up menu Figure 20 Procedure 1 Type in the password and click on the Enter New Password button You will be prompted with the following pop up button Figure 21 2 Turn on the light plate by briefly pressing on the Start button Insert it into...

Страница 18: ...nce OK is clicked the plate will be drawn into the GloRunner and the detectors will be standardized 4 If you see a message similar to Figure 23 and your light plate is powered on please contact Turner BioSystems for support Figure 23 ...

Страница 19: ...nks for both instruments It is not sufficient to compare the statistics on the blanks alone because the samples give a relative scale of the light units between different instruments If a detailed sensitivity study with multiple serial dilutions of the sample is not possible a common tool for determining rough sensitivity is CV CV is defined as the standard deviation of a group of samples divided ...

Страница 20: ...s are logged for technical support purposes Logging these values can be valuable in diagnosing instrument related performance issues Q Why is the calibration procedure password protected A Calibration of the detectors is only needed under rare circumstances We would like to be certain that you have spoken to a technical support person before you calibrate the detectors to be sure this will solve t...

Страница 21: ... Turner BioSystems GloRunner Microplate Luminometer software in the D drive of your computer Most computers will automatically start the installation process once the disk is placed in the CD ROM If your computer automatically installs you will see the InstallShield Wizard screen show in Figure 24 Figure 24 2 If your computer does not automatically start the installation process click the Windows ...

Страница 22: ...rectory for installation is C Program Files Turner BioSystems GloRunner To select an alternative directory click on the Change button shown in Figure 25 and choose your directory option as seen in Figure 26 Click the OK button when you are ready to continue with the software installation Figure 25 Figure 26 ...

Страница 23: ...mputer The GloRunner is powered on and the RS 232 is not connected to the computer v The red LED indicates other types of errors When the red LED is illuminated a potentially serious error has occurred At this point you should contact Turner BioSystems technical support for further instructions Firmware Upgrades The GloRunner Microplate Luminometer has the capability to download new firmware to th...

Страница 24: ...ely dry inside Warranty Turner BioSystems warrants the GloRunner Microplate Luminometer and accessories to be free from defects in materials and workmanship under normal use and service for a period of one year from the time of initial purchase with the following restrictions v The instrument and accessories must be installed powered and operated in compliance with the directions in this Manual an...

Страница 25: ... reimburse you for the cost of surface shipment to us v If you are an international customer who purchased directly from Turner BioSystems not from a third party distributor contact Turner BioSystems for instructions The instrument will be repaired at no charge if it is under warranty Turner BioSystems cannot however pay shipping duties or documentation costs outside the continental United States ...

Страница 26: ... assist you by phone or correspondence at no charge v Repair service will be billed on a flat rate basis Your invoice will include freight charges Address for Shipment Turner BioSystems 645 N Mary Ave Sunnyvale CA 94085 USA Telephone 408 636 2400 Toll Free 888 636 2401 US Canada Fax 408 737 7919 Email techsupport turnerbiosystems com ...

Страница 27: ...ght channel solid state array patent pending Spectral Response Range 300 610 nm Peak Wavelength 550 nm Detection Mode Glow luminescence Plate Format 96 well Reading Speed 1 second per well less than 30 seconds per plate Computer Interface RS 232 User Interface User friendly Windows software controls machine functions Requires Windows 95 or later Power 0 5A 100 240V 50 60Hz universal Dimensions 16 ...

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