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Portable Optical Tweezers Kit Chapter 5: Setup and Adjustment
Rev B, February 15, 2022
Page 51
Settings:
•
Press the
Settings
button in the Kinesis software and select the “Advanced” tab
(Figure 40).
• In the
Device Controls
field, choose “Velocity control” for the
Wheel Mode
(sometimes also referred to as Joystick Mode). This corresponds to a continuous
motion of the stage when the wheel is actuated.
•
The recommended
Max. Velocity
is set to 0.03 mm/s and
Acceleration
to 1 –
2 mm/s 2 .
•
The recommended
15 Drive Array Velocities
are set to
o
Velocity 4
: 0.03
o Velocity 3
: 0.02
o
Velocity 2
: 0.01
o Velocity 1 : 0.005
This defines the different velocities which can be accessed via drive buttons in
the software as shown in Figure 41. The controller wheel deflection is proportional
to the max. velocity.
• The settings need to be entered for each servo motor separately. All other
settings remain default values.
Important Note: By clicking OK, the settings are sent to the device. By checking the
Persist Settings to the Device
checkbox the settings are stored in the device and
loaded after each restart of the controllers. In the Kinesis Software you can also enter
all parameters under the
Device Startup Settings
tab. By clicking
Save
, this will store
the settings on your computer as well. When the controllers are reconnected to this
computer they will download the stored settings immediately. You can find further
information in the KDC101 manual.
You can operate the motors either by hand via the controllers or by software. When you
choose the latter, you can move the stage by pressing the “Drive” buttons in the main
window, see Figure 41. Here, you can also open the Drive Array Velocities in a drop-down
menu. Each of the horizontal blue bars in the “Drive” button field corresponds to one of the
velocities.
15
These velocities are good start values to ensure that the particles are not lost when moving at
maximum speed. If the Optical Tweezer is adjusted very well, trapping is possible at even higher
velocities.