ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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iSort
™
Automated Cell Sorter User Guide 21
3. Startup
Before you begin
Required solutions •
Sheath fluid: We recommend using sterile, flow cytometry grade 1X PBS as
the carrier for transporting particles through the optical cell.
•
100% bleach solution: 5.25% sodium hypochlorite in water. We recommend
using laboratory-grade bleach without additives and perfumes. Add 5 mL of
the 100% bleach solution to the Waste container to help prevent microbial
contamination.
•
10% bleach solution in deionized water: Used for decontaminating the
fluidics lines. Prepare this solution fresh daily and use during the Shut Down
procedure.
•
Detergent solution: Different detergent solutions are recommended for
different cleaning and maintenance functions.
-
We recommend using the Attune ™ Wash Solution (Cat. No. A24974)
when running the cleaning cycles (page 56) or performing the system
Shut Down (page 88).
-
For the storage of the nozzle tip after daily system Shut Down (page 88),
we recommend using 5% Decon
™ Contrad ™ 70 (Decon Laboratories, Inc.)
liquid detergent solution in deionized water.
-
We recommend using 5% Tween ™ 20 in deionized water when preparing
the Attune ™ Performance Tracking Beads for use in the daily performance
test (page 28).
•
Deionized water : Used in various cleaning and maintenance procedures, for
overnight storage of the instrument when not in use, and for preparing
various solutions.
IMPORTANT!
10% bleach is defined as a 1:10 dilution of 5.25% sodium hypochlorite
in deionized water. This gives a final concentration of 0.5% sodium hypochlorite
equivalent to 5000 ppm of available chlorine. We recommend using laboratory-
grade bleach. Avoid bleach with additives (such as perfumes).
IMPORTANT!
The use of the proper sheath fluid is essential for successful results.
Components such as sodium fluoride and phenoxyethanol can reduce cell
viability significantly and endotoxins, RNase, DNase, and proteases can hinder
further research procedures.
The use of non-sterilized sheath fluid is likely to result in contaminated cell
cultures. To reduce the risk of sheath contamination, make sure that the sheath
fluid container has been cleaned before adding sheath fluid.
Note:
Recommended storage temperature for solutions is room temperature
(15–30°C), but they can also be stored at colder temperatures. However, running
the instrument with cold reagents (<15°C) will affect the data quality. Before you
run the instrument, ensure that all fluid temperatures are at least 15°C.