ThermoFisher Scientific invitrogen iSort Series, Руководство пользователя

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28 iSort
™
Automated Cell Sorter User Guide
Run Performance Test
Overview The Performance Test procedure allows you to track the performance of the iSort ™
Automated Cell Sorter and verify performance against the QC criteria. The
procedure requires the use of the Attune
™
Performance Tracking Beads.
We recommend that you run the procedure every day to ensure that the system is
performing optimally before starting your experiments.
Required materials •
Attune ™ Performance Tracking Beads (Cat. No. 4449754). Before use, briefly
vortex the beads to mix.
•
5% Tween
™
20 in deionized water (Cat. No. 003005). Before use, dilute the
50% Tween ™ 20 solution to 5% in deionized water.
•
10X PBS
•
Sheath fluid (1X PBS, flow cytometry grade)
•
Deionized water
CAUTION!
The Attune ™ Performance Tracking Beads contain 0.05% sodium azide
as a preservative. Sodium azide is an extremely toxic and dangerous compound,
particularly when combined with acids or metals. Properly dispose of solutions
containing sodium azide.
Run Performance
Test
1. On the Maintenance screen, press Calibration & Performance Test , then select
Performance Test .