Thermo Scientific Vitrobot Mk IV, Руководство пользователя

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Chapter | Safety
Confidential, limited rights
PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 14
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Use a cryogenic glove and/or tongs to handle any object going into or out of the Liquid
Nitrogen.
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Dewar flasks are under vacuum to provide insulation and can collapse from thermal shock
or slight mechanical shock.
•
Before usage, the inside of the Dewar flask should be inspected for etched surfaces,
cracks or pinholes which could cause an explosion. When damage is observed the flask
should be replaced. If there is a small leak in the flask e.g. developed when it is frozen
and some liquid “enters” the flask vacuum compartment it will expand when the flask is
warmed up, (expansion ratio is 696:1) and cause an explosion.
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If cryogenic liquid or cold boil off contacts a worker's skin or eyes, frozen tissues should
be flooded or soaked with tepid water (60-100 F, 16-38 °C). DO NOT USE HOT WATER.
Cryogenic burns which result in blistering or deeper tissue freezing should be seen
promptly by a physician.
Note For more information about possible dangers, causes and recommen dations when
using a Dewar flask, see manufacturer’s safety instructions.
2.6 Climate Chamber
CAUTION! Heated surfaces. Extreme temperatures may be present. Take necessary precautions.
The Vitrobot chamber can be operated at a temperature range between 4 °C and 60 ° C; when
used at hot temperatures, care should be taken not to touch hot surfaces to avoid skin burn.
2.7 Safety Data Sheets (SDS)
For Safety Data Sheets, please refer to your chemicals supplier.
2.8 Biohazard
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When used in a ML-II (BSL-2) or higher category lab, especially when working with
infectious biological material, the Vitrobot pads can be protected to avoid contamination by
covering them with a piece of Parafilm M® plastic sheet, carefully placed between the
sponge material of the pads and the filter paper disc used for blotting the samples prior to
the plunging session.
•
After use, both the filter paper disc and the Parafilm M® sheet should be disposed
according to the EH&S rules and regulations in vigor depending on the BioSafety level and
local officer guidelines.