Thermo Scientific TSQ Altis, Руководство по оборудованию

Страница: 73 / 176

Thermo Scientific TSQ Altis, Quantis, and Fortis Hardware Manual 55
15
Calibrating the MS
Set up the syringe pump and the MS for calibration, and then perform the calibration.
• Preparing the Syringe Pump for Calibration
• Preparing the MS for Calibration
• Determining the Initial API Source Settings
• Evaluating the Spray Stability
• Calibration Parameters
• Calibrating and Tuning the MS
Preparing the Syringe Pump for Calibration
Use the syringe pump to infuse the EMRS calibration solution into the H-ESI source. For a
list of calibration supplies, see
Appendix E, “Replaceable Parts.”
Before you begin, follow the procedure in Appendix B, “Setting Up the Syringe with the
Syringe Pump.”
Risk of electric shock: This instrument uses voltages that can cause electric shock and /or
personal injury. While operating the instrument keep the covers on.
CAUTION Do not wear nitrile gloves when you are working with the calibration solution,
EMRS. Nitrile gloves are made with diphenylguanidine which is soluble in EMRS. If
diphenylguanidine contaminates the EMRS, a peak at
m/z 212 will be in the spectrum.
IMPORTANT To prevent the EMRS from degrading, do the following:
• Do not return any EMRS back to the original vial.
• Do not store EMRS in a glass syringe. Discard unused EMRS and rinse the syringe
with acetonitrile.
• If you observe degradation products in the EMRS mass spectrum, rinse the sample
line (including the fittings, syringe, tubing, and union) with acetonitrile.