Thermo Scientific TSQ Altis, Руководство по оборудованию

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15
Calibrating the MS
Calibration Parameters
60 TSQ Altis, Quantis, and Fortis Hardware Manual Thermo Scientific
b. Adjust the spray voltage plus or minus 250 units.
c. Change the API depth position from L/M to M.
8. Repeat this procedure for negative ion polarity mode.
Calibration Parameters
The calibration parameters affect mass accuracy and resolution.
The tune parameters affect the magnitude of the ion signal. There are two types of tune
parameters:
• Mass-dependent parameters: DC offset voltages of lens L0, Turner-Kruger Lens 1 (TK1),
and mass-analyzing quadrupoles and their end segments.
• Compound-dependent parameters: spray voltage (H-ESI or N-ESI), spray current
(APCI), sheath gas pressure, auxiliary gas pressure, sweep gas pressure, vaporizer
temperature, and ion transfer tube temperature.
Calibrating and Tuning the MS
Successful calibration of the MS requires a steady flow rate of EMRS.
Before you calibrate the MS, follow the procedure “ “Evaluating the Spray Stability.”
1. Set the syringe pump to infuse the EMRS at 5 μL/min into the API source.
2. Place the MS in On mode.
3. In the Define Scan pane, select Full Scan (Q1) or Full Scan (Q3) .
4. Set the scan range to 50-1650 .
Note When the MS completes a calibration, the Tune application writes the calibration
parameters and the mass-dependent tune parameters to the calibration file, which
overwrites the existing file. You cannot replace or modify the calibration file.
The Tune application writes the compound-dependent tune parameters to a change
record in the History pane. You can rename and save the change record to the Favorites
pane for future use in the Tune application or the Method Editor.
CAUTION Do not operate the system if it fails a calibration procedure. Contact a Thermo
Fisher Scientific field service representative.
Note
• For optimum performance, calibrate the MS every one to three months of operation.
• To reduce the risk of potential errors, monitor quality control standards frequently.