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USER GUIDE

For Research Use Only. Not for use in diagnostic procedures.

CONFIDENTIAL — For Life Technologies Internal Use Only. Do Not Distribute.

Ion Torrent S5

™

and Ion Torrent S5

™

Sequencer

Catalog Numbers

A27211, A27213

Publication Number

MAN0010811

Revision

A.0

Содержание Torrent S5 XL

Страница 1: ...nly Not for use in diagnostic procedures CONFIDENTIAL For Life Technologies Internal Use Only Do Not Distribute Ion Torrent S5 and Ion Torrent S5 XL Sequencer Catalog Numbers A27211 A27213 Publication Number MAN0010811 Revision A 0 ...

Страница 2: ...IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF Important Licensing Information These products may be covered by one or more Limited Use Label Licenses By use of these products you accept the terms and conditio...

Страница 3: ...tions 11 Ion Torrent S5 and Ion Torrent S5 XL Sequencer input and output connections 12 CHAPTER 3 Create a Planned Run 13 About planned runs 13 Create a planned run 13 Planned run wizard key fields 14 CHAPTER 4 Instrument operation 16 Power the Ion Torrent S5 or Ion Torrent S5 XL Sequencer on or off 16 Power on 16 Power off 16 Chip handling guidelines 16 Select the planned run and perform the run ...

Страница 4: ...fuge 32 APPENDIX D Safety 34 Symbols on this instrument 34 Conformity symbols on this instrument 35 Safety alerts on this instrument 36 Location of safety labels on this instrument 36 Safety information for instruments not manufactured by Thermo Fisher Scientific 37 Instrument safety 37 General 37 Electrical 37 Cleaning and decontamination 38 Safety and electromagnetic compatibility EMC standards ...

Страница 5: ...ix in this document Revision history Revision Date Description A 0 November 2014 Ion Torrent S5 and Ion Torrent S5 XL Instrument User Guide for compliance testing Purpose of the guide The Ion Torrent S5 and Ion Torrent S5 XL Instrument User Guide Pub no MAN0010811 provides reference information for using the Ion Torrent S5 or Ion Torrent S5 XL Sequencer s Cat nos A27211 and A27213 Ion Torrent S5 a...

Страница 6: ...orrent S5 XL System Catalog no A27214 Components Catalog no Ion Torrent S5 XL Sequencer A27213 Ion S5 Installation Kit A27215 Ion S5 Installation Kit Catalog no A27215 Components Color Quantity Volume Storage Ion 520 530 OT2 Kit 4 2 C to 8 C Ion 540 Chip Kit 4 15 C to 30 C Ion Torrent S5 Sequencing Kit 4 2 C to 8 C Ion S5 Controls Kit 1 2 C to 8 C 1 6 Ion Torrent S5 andIon Torrent S5 XL Instrument...

Страница 7: ...Tubes Flat Cap do not use polystyrene tubes Axygen PCR 02 L C Varies 1 5 mL or 1 7 mL microcentrifuge tubes MLS Varies Ice buckets and ice Aluminum heat block capable of maintaining 50 C MLS Vortex mixer with a rubber platform MLS 1 Thermal cycler with a heated lid MLS 1 Standard laboratory vacuum line or vacuum pump MLS 1 Liquid trap MLS 1 Tygon tubing 4 MLS 1 1 Thermo Fisher Scientific has valid...

Страница 8: ... add noise and reduce the quality of the measurements The Ion Torrent S5 System must be installed on a bench that is free from vibrations or in contact with equipment that can cause vibrations to the bench freezers pumps and other similar equipment IMPORTANT Place the instrument at least 40 in 1 meter away from major sources of electronic noise such as refrigerators or microwaves Static electricit...

Страница 9: ...rrent S5 System must be connected to the Ion Torrent Server by a standard Category 6 Ethernet cable We do not troubleshoot data transfer issues associated with an indirect connection between the Ion Torrent S5 XL Sequencer and the Ion Torrent Server Torrent Browser 3 4 5 6 2 1 Figure 1 Ion Torrent S5 XL Sequencer configuration 1 Ion Torrent S5 XL Sequencer 2 Ion template preparation instrument Ion...

Страница 10: ...her Scientific field service engineer to correct the problem at your own expense In providing outbound access to the internet from the server you enable the Thermo Fisher Scientific support team to provide inbound support Both the Ion Torrent S5 Sequencer and the Ion Torrent Server run a remote monitor agent that can provide service personnel with critical system information such as installed soft...

Страница 11: ... 7 1 2 3 5 6 4 1 Touchscreen 2 Reagent cartridge 3 Power button 4 Chip clamp 5 Primary Wash solution bottle W2 position Waste reservoir located behind the W2 bottle shown on the right 6 Secondary Wash solution bottle W3 position 7 Waste reservoir Note The system utilizes RFID technology to verify that the proper reagents are loaded in positions 2 5 and 6 Reagents that exceed their expiration date ...

Страница 12: ...th the Ion Torrent S5 or Ion Torrent S5 XL Sequencer 3 On off switch Power switch where the states are on or off O 4 Power port 100 240VAC port that provides power to the instrument Chapter 2 System components Ion Torrent S5 and Ion Torrent S5 XL Sequencer input and output connections 2 12 Ion Torrent S5 andIon Torrent S5 XL Instrument User Guide CONFIDENTIAL For Life Technologies Internal Use Onl...

Страница 13: ...dy to perform the run see t_Start the sequencing run Note For additional information see the Torrent Suite User Interface Guide available on the Ion Community at ioncommunity lifetechnologies com Create a planned run 1 Open Torrent Browser for the Ion Torrent Server connected to your Ion Torrent S5 or Ion Torrent S5 XL Sequencer 2 Select the Plan tab select Templates locate the application you wan...

Страница 14: ...ressRNA barcode set which contains all 16 barcodes in the Ion Xpress RNA BC01 16 Kit If you are not using barcodes with DNA libraries Leave the Barcode field blank RNA libraries prepared using the Ion Total RNA Seq Kit v2 Select RNA_Barcode_None from the drop down list This will ensure that the proper trimming is performed on the resulting sequence when the RNA library does not have a barcode Plug...

Страница 15: ...hresholds Set thresholds for Bead Loading Key Signal and Usable Sequence In the Ion Proton Torrent Browser Monitor4Runs in Progress tab an alert is displayed if the values for a run fall below the selected thresholds Chapter 3 Create a Planned Run Planned run wizard key fields 3 Ion Torrent S5 andIon Torrent S5 XL Instrument User Guide 15 CONFIDENTIAL For Life Technologies Internal Use Only Do Not...

Страница 16: ...ry to power off the instrument overnight or over the weekend If the instrument will not be used for more than 3 days power off the instrument as follows 1 In the Main Menu select Tools Shut Down 2 Ensure the post run cleaning is complete then press Shut Down 3 After you exit the main touchscreen press the Halt button then OK when prompted The instrument will power down Chip handling guidelines To ...

Страница 17: ...g this procedure 1 Secure the chip loaded with template positive Ion Sphere Particles in the chip clamp close the instrument door then press Next 2 In the drop down list select a planned run that you created in the Torrent Suite Software then press Next Note You can also select Planned Run none then enter your run information on the following screen but selecting a predefined planned run is recomm...

Страница 18: ...Press the red pop up to see the detailed messages If a message states New Software Available update the software as follows Reviewer How do users with instruments not connected to the internet receive notification that software updates are available and how do they update the software 1 In the Main Menu select Options4Updates 2 Select the Released Updates checkbox then press Check 3 When the messa...

Страница 19: ...ottle then remove and empty the waste reservoir 2 Remove the nucleotide reagent cartridge 3 Visually inspect the waste and nucleotide reagent bays for liquid 4 Using absorbent paper soak up as much liquid as possible then wash the affected area with 10 bleach solution 5 Wipe the affected surfaces with IPA wipes then allow to air dry Chapter 4 Instrument operation Clean the instrument 4 Ion Torrent...

Страница 20: ...be performed before each run to prepare the run reagents The Initialize program walks you through the following steps Specifying the sequencing kit Loading the reagent and wash solutions After this step the instrument performs a reagent check Abbreviated instructions are provided on the touchscreen for detailed instructions see The Run program walks you through steps leading up to and through sequ...

Страница 21: ...iew by support personnel For troubleshooting if directed to do so by Technical Support Reagent Check Measures the pH of all reagents on the instrument This task is normally performed by the instrument as part of the Initialize program If directed to do so by Technical Support or as part of a troubleshooting procedure see Appendix B Troubleshooting RFID Lists the product product expiration date and...

Страница 22: ...ulator pressure If the icon is red see Manifold temperature If the icon is red see Chip block temperature If the icon is red see Percent instrument hard drive and SSD in use If the icon is red see LAN local area network status Icon turns red when instrument is not connected to the network Instrument status Green shown ready to use Red pulsing in use Appendix A Touchscreen reference Touchscreen gau...

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