Section 9- Proteins & Labels
Max Absorbance:
used to rescale the upper limit of the vertical axis.
Sample Type:
The same six sample types (options) listed under Protein A280 (Section 8) are available for purified Proteins & Labels
analysis and concentration measurement. All of the options can be viewed by clicking the mouse while it is positioned within the
sample type box. The sample type (color-keyed) can be selected by clicking on the preferred option or by scrolling through the
selections using the up or down arrow keys located to the left of the sample type box. See Protein A280 (Section 8) for a detailed
description of each sample type.
λ
3:
user-selected wavelength
Norm Abs @
λ
3:
10mm equivalent normalized absorbance at the respective wavelength.
Dye 1 (or 2):
user-selected dye
Norm Abs @
λ
1:
normalized 10 mm equivalent absorbance of selected Dye
uM:
concentration based upon selected Dye’s extinction coefficient. See the “Concentration Calculation (Beer’s Law)” Appendix for
more details on this calculation.
Abs ratio
λ
1/
λ
3:
ratio of the absorbance of Dye 1 to the absorbance at the user-selected wavelength (
λ
3).
mg/ml:
concentration of proteins in the sample calculated using the absorbance at 280 nm minus the absorbance at 340 nm (i.e.
normalized at 340 nm). See the “Concentration Calculation (Beer’s Law)” Appendix for more details on this calculation.
Show Report:
formatted for 200 samples although the buffer size can be increased to accommodate thousands of samples.
Baseline Type
This application module has two user-selectable “Baseline Type” options. The default setting is set to normalize the display spectrum at
750nm. Alternatively, the 400-750 Slope Baseline Type will normalize the display at 750nm and accommodate any linear baseline offset
across the 400 to 750nm range.
9-2
