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Programming and Operation

C.3.5 Supernatant Collection

Example:

Aspirate 100 µl from 8 rows of a Microtiter

plate and discard the

total volume.

This program is performed with the 1250 µl
Equalizer pipette. All 100 µl fill volumes are
collected in the same tips. When completed, the
total volume is discarded with the purge step.

C.4 Operating Procedures

After programming the pipette, the display should show the first program step,
with the “Run” prompt showing in the lower left corner:

Figure C.4: Run Prompt

The following steps describe a basic program for pipetting reagent into a
Microtiter plate. The pipette is programmed as follows:

[Fill]

Vol

(250) [Enter]

Note:

The 10 µl Primer

Dispense reduces the
percentage of error in
incremental dispensing
(see section D.3
Recommendations for
Accurate Pipetting)

[Disp]

Vol

(10) [Enter]

[Disp]

Vol

(30) [Enter] [Enter]

[Fill]

Vol

(100) [Enter]

[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]

[Purge] [Enter] [Enter]

Содержание Matrix MultiChannel Equalizer Pipette

Страница 1: ...Thermo Scientific Matrix MultiChannel Equalizer Pipette with Expandable Equal Tip Spacing User Manual ...

Страница 2: ...pe 44 161 0 4862110 email matrix eu info thermofisher com Asia Pacific Local representative or matrix ap info thermofisher com 2011 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries All other trademarks and product names are the intellectual industrial property of their respective owners ...

Страница 3: ...s and Precautions 11 C PROGRAMMING AND OPERATION 12 C 1 Operating the Equalizer Pipette 12 C 2 Programming the Equalizer Pipette 14 C 2 1 Open Programming Mode or Scratch Pad P 0 14 C 2 2 Entering a Program in Open Programming Mode 14 C 2 3 Storing a Program 17 C 2 4 Program Recall 17 C 2 5 Adjusting Pipetting Speed 18 C 2 6 Reviewing the Program 18 C 3 Programming Examples 19 C 3 1 Sample Transfe...

Страница 4: ...r Pipette Maintenance 33 F 1 1 Equalizer Pipette Disassembly 33 F 1 2 Equalizer Pipette Cleaning and Autoclaving 36 F 1 3 Equalizer Pipette Maintenance and Reassembly 36 F 2 Installing Replacing Batteries 42 F 3 Charging Recharging Batteries 44 G TECHNICAL SERVICE 46 G 1 Instrument Warranty 46 G 2 Recommended Equipment Decontamination Methods 47 G 3 Factory Service Calibration Maintenance Repair 4...

Страница 5: ...IV ...

Страница 6: ...tasks quickly accurately and with less risk of developing repetitive strain injuries RSI typically associated with manual pipette use Laboratory results will become more standardized while less time is expended on tedious bench procedures Before You Begin Before using the Matrix Equalizer pipette verify the contents of your package The package should contain the following items 1 Matrix Equalizer ...

Страница 7: ...spirating and dispensing of viscous liquids Ideal for gel loading EASY CAL simplifies in lab calibration verification using distilled water The Matrix Equalizer pipette is lightweight and cordless utilizing a rechargeable battery that can be used during recharge The pipette also has an automatic shut off feature to extend battery life The Equalizer pipette uses integrated circuit technology to sto...

Страница 8: ...h 8 and 12 channnels Models range in volume from 0 5 µl to 1250 µl Each handle is color coded with a color ring for easy volume identification see section B 5 Handle Specifications B PIPETTE OVERVIEW B 1 Components Figure B 1 eQualizer Components 8 channel Equalizer Pipette 8 channel Equalizer 384 Pipette 12 channel Equalizer 384 Pipette ...

Страница 9: ...m A single pipetting step is executed by a quick press and release of the trigger Repeat pipetting is executed by pressing and holding the trigger Note At the end of any dispense the pipette tips should be removed from the fluid before releasing the trigger to prevent aspiration of fluid Ejector Trigger The ejector trigger is pressed to eject the tips from the pipette Tip Fittings The tip fittings...

Страница 10: ...rogrammed for the first time the first step will be the fill operation with the default fill volume See Section C 1 Programming the Equalizer Pipette for the default settings Prog The Program key on the Matrix Equalizer pipette enables you to store up to five separate programs in memory for future recall Each program can hold up to 40 steps Programs are stored in reserved program location numbers ...

Страница 11: ...ode until you program a new pipetting sequence B 3 2 Programming Keys Fill Disp Mix The Fill Dispense and Mix keys program the pipetting operations By pressing fill or mix keys you can enter the programming mode of the Equalizer The Run prompt disappears from the display and the appropriate operation appears with a flashing Vol prompt Fill dispense or mix volumes can be programmed by pressing the ...

Страница 12: ...erifies the end of the program with a triple beep tone B 3 3 Special Function Key The Special Function key is the small hole below the Volume key It can be activated with a pipette tip By repeatedly pressing the Special Function key you can display four functions Pipetting Speed SPd Beep Tone TONE Paced Dispense Speed PACE and Calibration CAL To exit the Special Function key at any time press the ...

Страница 13: ...ep tone symbol appears at the bottom of the display Note For paced dispensing the beep tone should be set ON1 to prepare you for each dispense step PACE The pace controls the speed of repeat pipetting paced dispensing FAST SLOW or OFF Press the Special Function key three times to display PACE and its current setting To change the setting press the Volume key Paced Dispense When using the paced dis...

Страница 14: ...he pipette display is a dynamic LCD screen It displays the current operation volume prompt if entering a program pipetting volume beep tone symbol if the beep tone is on pipetting speed and program number Figure B 4 Equalizer Display ...

Страница 15: ...1 0 µl 1 0 0 6 µl 2239 12 50 µl 12 Ch 384 0 50 12 50 µl Red 2 5 0 15 µl 2 0 0 15 µl 2230 30 µl 12 Ch 384 1 0 30 0 µl Purple 2 0 0 30 µl 1 7 0 30 µl 2231 125 µl 12 Ch 384 2 0 125 0 µl Yellow 2 0 1 0 µl 1 2 0 8 µl Table B 5 Equalizer Pipette Specifications Note For Accuracy and Precision values shown are expressed as a percent deviation or microliter µl value When applied to desired volume the great...

Страница 16: ... of an incompatible power transformer can damage the pipette CAUTION Always turn off the power and unplug the power transformer before cleaning the exterior Fluid seepage can damage internal components CAUTION Do not immerse the unit in liquid Fluid seepage can damage internal components CAUTION Do not autoclave the entire unit Extreme heat can damage the display and other electronic components No...

Страница 17: ...d position A In the case of the Equalizer 384 pipette with two set buttons tips arranged in racks of 384 can be attached when the indicator is set at the 4 5 mm mark Once tips are attached follow the steps below to change the pipette to a new tip spacing Matrix Equalizer Pipette 1 Depress the set button using your thumb or finger 2 While depressing slide the set button to the desired tip to tip sp...

Страница 18: ...n using your thumb or finger and repeat steps 2 and 3 Note On 8 Channel Equalizer 384 pipettes there is a notch see figure C 1 Position C located on the control rod that indicates the 9 mm position for easier aligning of the control rod for 96 well plates By applying pressure to the front side of the control rod the notch can be by passed for smoother slide operation Conversely when pushing or pul...

Страница 19: ... affecting previously stored programs To use the scratch pad mode From the start of any program simply begin entering a new protocol selecting a pipetting operation as described below This new protocol can now be used and will remain active until a stored program is retrieved P 0 appears in the program ID section of the display to indicate that the O pen programming mode or scratch pad mode is act...

Страница 20: ... can be the entire fill volume or a smaller increment for repeat pipetting The dispense volume needs to be programmed only once for repeat pipetting The pipette will dispense the volume each time the trigger is pressed or repeatedly during a paced dispense until the fill volume is reached or until insufficient volume remains Mix The Mix key programs the pipette to perform a combination of fills an...

Страница 21: ...is not a mix cycle the Purge prompt automatically appears Also the pipette emits a double beep tone to warn you that some fluid remains Press the trigger to dispense the remaining fluid and return to the beginning of the program Examples of pipetting programs with different mix steps are shown in Section C 3 Programming Examples 3 Enter volume Enter the desired volume for Fill Disp or Mix by press...

Страница 22: ...gram number P1 P5 is displayed 2 Press and hold the Prog key while simultaneously pressing the Special Function key to enter the programming mode The prompt Prog will appear on the display 3 Begin entering your new program refer to Section C 2 2 Entering a Program in Open Programming Mode for instructions NOTE When programming mode is active the Speed Setting and Beep Tone symbols will blink Upon ...

Страница 23: ...m functions until changed by the operator C 2 6 Reviewing the Program After programming the pipette review the programming steps 1 Set pipette for RUN mode Ensure the pipette is in the RUN mode RUN appears in the lower left corner of the display If the display is flashing all pipetting operations it is still in the programming mode press Enter to save the program and exit 2 Begin program Run the p...

Страница 24: ... Fill and dispense 100µl Option 1 Option 2 Fill Vol t s 100 Enter Fill Vol t s 100 Enter Enter Disp Vol t s 100 Enter Enter The second option is a one step program Because the program ends at a volume greater than zero and the last step is not a mix a Purge prompt automatically appears at the end of the fill to dispense the liquid and return to the beginning of the program C 3 2 Incremental Pipett...

Страница 25: ...ith reagent Mix 200 µl for 2 cycles Transfer 100 µl to the second column and repeat the mix step Follow this procedure for the rest of the plate Fill Vol t s 100 Enter Mix Vol t s 200 Mix Vol t s 2 Enter Enter Enter That s the entire program The mix step always returns to the volume at which it started 100 µl therefore the pipette continues the mix step indefinitely until you press the Purge key t...

Страница 26: ...he display should show the first program step with the Run prompt showing in the lower left corner Figure C 4 Run Prompt The following steps describe a basic program for pipetting reagent into a Microtiter plate The pipette is programmed as follows Fill Vol t s 250 Enter Note The 10 µl Primer Dispense reduces the percentage of error in incremental dispensing see section D 3 Recommendations for Acc...

Страница 27: ...of the eighth dispense the pipette emits a double beep tone if the beep tone is on otherwise it emits a single beep tone and displays the Purge prompt There is insufficient volume for another 30 µl dispense and the pipette signals for a purge step or Paced Dispense Steps 4 and 5 can also be performed as a paced dispense Instead of pressing the trigger for each dispense step press and hold the trig...

Страница 28: ... not limited to this volume D 1 Volume Measurement To measure the actual value of the programmed volume 1 Fill the programmed volume Vp then dispense the entire volume into a container on the analytical balance 2 Read the weight on the analytical balance Repeat this procedure ten times Note Use a clean and dry pipette tip with each pipetting cycle When filling the liquid immerse the pipette tip ap...

Страница 29: ...eport Please use the step listed there for best results If you cannot locate you documentation that lists the factory calibration step contact Thermo Fisher Scientific Tech Support at 800 345 0206 and have your pipette s serial number ready D 2 2 EASY Cal Mode Equalizer pipette users can quickly calibrate their pipette by using the unique EASY CAL feature EASY CAL has been developed for fast easy ...

Страница 30: ...r by pressing the Enter key The new calibration setting is confirmed by the CAL and SET messages on the display 5 Verify this new calibration setting by repeating Steps 1 and 2 D 2 3 CALC Mode To calibrate the pipette 1 Weigh out ten aliquots of distilled water on an analytical balance using one pipetting channel The programmed dispense volume must correspond to the calibration point for that pipe...

Страница 31: ...ibrate a 1250 µl 8 Channel pipette Water temperature 24 C Programmed Volume Vp 1000 µl Average of Actual Volume 990 µl Corrected Actual Volume Va 990 x 1 004 994 µl Calibration Point Cp 1000 DV x Cp Vp Va DV x 1000 1000 µl 994 µl DV 1006 D 2 4 CALVIEW CALVIEW displays the pipetting stroke number of steps used to reach the calibration point This value allows you to confirm the calibration during pi...

Страница 32: ...with air pressure relative humidity and the liquids vapor pressure all of which are temperature dependent Attach tips with slow even pressure When applying tips avoid banging or applying excessive force to the tip s as this stretches the tip seal and will affect volume Use Matrix tips which have been specifically designed to match the tip fittings on Matrix pipettes Calibrate the pipette for the l...

Страница 33: ...ueous solutions at faster speeds to expel liquid more accurately Note Viscous liquids should be dispensed slowly Tip touch During the dispensing of a liquid touch the tip against the side of the receiving container to prevent any liquid from remaining on the tip Discard final dispense When accurate dispensing of several increments of liquid are needed discard the last increment The cumulative perc...

Страница 34: ...ch as programming two fills that exceed the maximum fill volume Recheck the programming sequence Ensure you are not attempting to fill above the maximum fill volume of the pipette or attempting to dispense more than the total fill volume ERR_1 is displayed Signifies one of the following conditions Probable Cause Recommended Solution Liquid is aspirated into the pipette Disassemble and clean the pi...

Страница 35: ...embly the pipette leaks or does not fill liquid Probable Cause Recommended Solution O ring was not properly seated Disassemble the pipette and reseat the O ring See Section F 1 and or F 2 for pipette disassembly reassembly O ring was damaged during reassembly Replace the O ring See Section E 2 2 Pipette Cleaning and Autoclaving Relubrication step and Section E 2 3 Pipette Reassembly Tubing was dis...

Страница 36: ...elow In cases where pipettes have been exposed to biohazardous materials autoclaving internal components may be required Thermo Fisher Scientific provides the following Preventive Maintenance Autoclave Kit Preventive Maintenance Autoclave Kit Replacement cross tubes O ring lubricant Wooden applicator O rings CAUTION DO NOT AUTOCLAVE entire unit or clean the keypad with bleach solution or other sol...

Страница 37: ...y 1 Remove the color ring by gently prying loose one of the edges near the handle trigger 2 Use a Phillips head screwdriver to remove all three housing screws 3 Carefully remove the plastic cover housing and observe the layout of the internal parts Figure F 1 1a Removing Cover Housing ...

Страница 38: ...tor Trigger and Ejector Comb 5 Remove the manifold assembly and piston cylinder assembly by pulling the motor screw down from the motor shaft coupling and out of the main housing Be careful not to misplace the motor screw washer Remove the manifold and piston cylinder assemblies by firmly lifting them out of the main housing support ribs see Figure F 1 1c Figure F 1 1c Removing Piston Cylinder and...

Страница 39: ... box 18 Battery 5 Driver board 19 Main housing 6 Motor 20 Trigger hinge pin 7 Color ring 21 Handle trigger 8 Motor coupling 22 Handle trigger spring 9 Motor screw 23 Ejector trigger 10 Piston backplate 24 Piston 11 Photosensor 25 O ring 12 Cylinder 26 Cross tube 13 Cover housing 27 Manifold plate 14 Housing screw Figure F 1 1d Pipette Components ...

Страница 40: ... Replacement The O rings may require replacement if liquids have been over aspirated In this case the O rings should be removed prior to autoclaving Spare O ring packs are available from Thermo Fisher Scientific for all pipette volumes 1 Remove old O rings 1250 μl 250 μl Pipettes O rings can be removed from the pistons by squeezing one side of the O ring and lifting it out of the O ring groove on ...

Страница 41: ...Autoclave Kit Figure F 1 3a Removing O rings 3 Re lubrication 1250 μl and 250 μl Pipettes Use the wooden applicator and O ring lubricant supplied in the Preventive Maintenance Autoclave Kit to apply a thin layer of lubricant approximately 0 5 inches approx 1 3 cm down into each piston hole that requires it Spread the lubricant evenly along the inner wall of each cylinder by using a circular motion...

Страница 42: ...of the cylinder see Figure F 1 4a NOTE To correctly orient the piston and cylinder assembly ensure that the lot number of the cylinder is facing up and the painted section of the piston backplate is facing down Also care should be taken to keep the O rings from being pinched when inserting the pistons into the cylinder Figure F 1 4a Reassemble Piston Cylinder Assembly 2 Cross Tube Replacement Conn...

Страница 43: ...le of the motor coupling This can be accomplished by holding the piston cylinder assembly at a slight angle while inserting the motor screw into the hole of the motor coupling Slowly spin the motor screw until the flat end of the motor screw is fully engaged into the motor coupling hole see Figure F 1 4c The cylinder can now be seated between the plastic ribs in the main housing NOTE The lot numbe...

Страница 44: ...t is the only tube not twisted Note The Manifold Assembly is comprised of the Manifold Plate and Tip Fittings and is connected to the Piston Cylinder Assembly by the tubing Figure F 1 4d Rotating Manifold Assembly 5 Installing Manifold Assembly Place the back plane divider onto the link assembly then insert the manifold assembly into the main housing support ribs Be sure to inset the pin located o...

Страница 45: ...with reassembly of the main housing and housing cover 6 Inserting Ejector Trigger Place the end of the ejector spring against the plastic stop and compress the spring while positioning the ejector trigger onto the ejector trigger rail NOTE Be careful not to pinch the cross tubes when inserting the ejector trigger Optional Dowel Back Plane Divider Manifold Assembly ...

Страница 46: ...mb Slide the ejector comb over the tip fittings with the slots on the back of the comb exposed the ribs on the ejector trigger should mate with the slots on the aluminum ejector comb see Figure F 1 3g Figure F 1 4g Replacing Ejector Comb Ejector Trigger Ejector Comb Ejector Comb ...

Страница 47: ...nd attach the housing cover Place the cover housing onto the main housing and fasten the three screws Figure F 1 4h Replacing the Housing Cover 9 Test Pipette Test the pipette for smooth operation repeat steps 4 9 as necessary 10 Replace Color Ring Re attach the color ring The pipette is now ready for use Use dowel to line up screw hole Housing Cover ...

Страница 48: ...ette is turned off before removing the battery case 1 Remove battery case Position your thumb over the thumb grips see Figures F 2a b and brace your fingers against the front of the keypad Apply pressure to the panel with the thumb grips to release latch Carefully slide battery case out of the pipette allowing sufficient slack for attached wires of the battery Figure F 2a Battery Case Closed Figur...

Страница 49: ...tor with a small flathead screwdriver Grasp all three wires and pull out connector see Fires F 2f g CAUTION Be careful not to tear the Flex Cable Figure F 2f Lift Battery Connector Figure F 2g Unclasp Battery Connector 5 Connect New Battery Attach the wires of the new battery in place of the old battery 6 Reassemble Console Cover Attach the pipette top to the main housing Fit the edges of the pipe...

Страница 50: ... current into the battery Flashing Orange to Green When the battery is fully drained the indicator will remain a solid orange for a while as the battery requires constant charge current As the unit continues to charge current is supplied in pulses This is indicated by the flashing of the indicator As the battery nears full charge the flashing slows down becoming nearly a constant green When the ba...

Страница 51: ...isassembled other than for procedures for normal maintenance No other warranty is expressed or implied including the warranty of merchantability Should a unit need to be returned the purchaser must pay insurance and shipping charges Thermo Fisher will assume the cost of returning the repaired unit to the purchaser This product packaging is capable of withstanding normal shipping hazards Contact Th...

Страница 52: ...onent Heat at 65 C 10 Hr 70 30 IPA Water 10 Bleach Ethylene Oxide Autoclave 121 C 15 min Multichannel Pipettes Pipette Exterior YES Wipe Wipe YES NO Piston Cylinder Assembly YES YES NO YES YES Tip Manifold YES YES NO YES YES Battery NO Wipe NO NO NO Power Supply NO Wipe NO NO NO Pipette Exterior NO Wipe Wipe NO NO Single Channel Electronic Pipettes Pipette w Battery Removed YES Wipe Wipe YES NO Lo...

Страница 53: ...nience with a single documentation form to select service options and certify proper decontamination Free shipping via FedEx ensures pipettes arrive at our facility quickly reducing the time away from your lab Contact your sales representative or call 800 345 0206 US or 44 0 161 486 2112 Europe to have these pre paid pipette shipping boxes delivered right to your door Accuracy Precision Every pipe...

Страница 54: ...lidation sheet or CD containing results of performance validation Validation If a pipette fails validation on site we will offer to repair it at our facility and if necessary deal with the original manufacturer to ensure any major repairs are completed properly Service and Support Our support specialists work in partnership with you to keep your pipettes performing at their peak We understand how ...

Страница 55: ...e approvals and marks mentioned Transformer Item 6099 6096 9068 6098 9068 6098 9068 Standard EN61010 1 1993 Safety regulations EN55022 EN55011 1 Emissions EN50082 1 1992 Immunity EN60825 DENAN Directive 73 23 EEC Low Voltage 89 336 EEC Electromagnetic compatibility Law No 234 Electical Appliance and Material Safety Law Japanese Ministry of Economy Trade and Industry Approval mark Europe ...

Страница 56: ...Key 7 F Factory Calibration Step 24 Fill 15 Fill Key 6 H Hazards and Precautions 11 K Keypad 5 L Lubrication 36 M Maintenance 31 Maintenance Procedures 31 45 Mix 15 Mix Key 6 O On Off Key 5 Open Programming Mode 14 Operation Keys 5 P PACE 8 Paced Dispense 8 22 Pipetting Trigger 4 Prog Key 5 Program Examples 19 21 Program Recall 17 Program Storing 17 Program Keys 6 Purge 16 Purge Key 6 R RUN mode 6...

Страница 57: ...52 Index ...

Страница 58: ...thermofisher com Hours of Operation 8 00 AM to 5 00 PM ET Europe Tel 44 0 161 486 2112 Email matrixtechcorp eu service thermofisher com Hours of Operation 08 30 to 17 00 GMT www thermoscientific com 25403 Rev F Thermo Fisher Scientific Inc All Rights Reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change ...

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