Thermo Scientific KingFisher Pure RNA Blood Kit, Инструкция по эксплуатации

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6 | Thermo Scientific KingFisher Pure RNA Blood Kit Thermo Fisher Scientific
Chapter 1 | Kit Content
an efficient purification. For more details, refer to Chapter 5: “Protocols and
Pipetting Instructions” . Suitable consumables for the KingFisher Duo and
KingFisher Flex are listed in Table 1-3 and Table 1-4 .
Storage Conditions
Upon arrival of the kit, store the DNase I, DNase I Reconstitution Buffer,
and 2 x DNase Buffer at -20°C. Also after dissolving of the DNase I,
continue to keep it at -20°C. The Proteinase K solution is stable at room
temperature until the seal of the vial is broken. After being opened, the vial
should be stored at -20°C. Store the Thermo Scientific™ KingFisher™
Magnetic Beads at +4°C. Other kit components can be stored at room
temperature (15−25°C). The reagents are stable for up to two years from
the manufacturing date.
Additional Reagents Required
• 96−100% ethanol (EtOH), molecular biology grade
• 100% isopropanol, molecular biology grade
• 2 M DTT (dithiothreitol) solution (store at -20°C)
• PBS (Phosphate buffered saline for Tempus™ protocol)
Table 1-2. Thermo Scientific™ KingFisher™ magnetic particle processors
Cat. No. Product
5400100 KingFisher Duo magnetic particle processor
5400630 KingFisher Flex magnetic particle processor with 96 deep well head
Table 1-3. Thermo Scientific™ KingFisher™ Flex consumables
Cat. No. Product Package size
97002534 KingFisher Flex 96 tip comb for deep well magnet 100 pcs
97002540 KingFisher Flex 96 KF plate (200 µl) 48 pcs
95040450 Microtiter deep well 96 plate 50 pcs
95040460 Microtiter deep well 96 plate, sterile 50 pcs