Thermo Scientific KingFisher Pure RNA Blood Kit, Инструкция по эксплуатации

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22 | Thermo Scientific KingFisher Pure RNA Blood Kit Thermo Fisher Scientific
Chapter 5 | Protocols and Pipetting Instructions
Make sure that the elution strip is placed in the correct direction into the
elution block. Ensure that the perforated end is facing towards the user.
When the KingFisher Duo is to be run as a standalone instrument, transfer
the PURE_RNABlood_Duo protocol to the KingFisher Duo. The instructions
for transferring the protocol can be found in Chapter 4: “Using the software”
in the
BindIt Software for KingFisher Instruments version 3.2 User Manual.
6. Add the KingFisher Magnetic Beads and isopropanol to row C during the
dispense step.
When the KingFisher Duo pauses at the first dispense step after the lysis
step at approximately 5 minutes after starting the protocol run, remove the
plate from the instrument, and add the KingFisher Magnetic Beads and
isopropanol to
row C on the Blood RNA plate .
Row Row name Add
Added reagent
volume per well
C Sample Isopropanol
KingFisher Magnetic
Beads*
400 µl
15 µl
* Resuspend the KingFisher Magnetic Beads well by vortexing before use.
7. Place the plate back into the instrument and press
OK
.
8. Add the Rebinding Buffer (supplemented with ethanol) to row A during
the dispense step.
When the KingFisher Duo pauses at the dispense step after the lysis step
at approximately 10 minutes after the first dispense step, remove the plate
from the instrument, and add the Rebinding Buffer into
row A on the Blood
RNA plate
to rebind the RNA.
Row Row name Add
Added reagent
volume per well
A DNase Rebinding Buffer 490 µl
9. Place the plate back into the instrument and press
OK
. After the pause,
the protocol will continue to completion.
10. After the run is completed, remove the plate and elution strip, and store
the purified RNA.