Thermo Scientific Invitrogen PowerEase Touch PS0350, Руководство пользователя

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7.
Touch Start run to begin the run. When the run begins, the Run screen will
display (See Figure 12).
Note: If the run needs to be stopped or paused before completion, touch Stop
or Pause . If run parameters need to be edited during the run, pause the run,
enter changes, then touch Resume . When resumed, the run time will continue
from where it was paused.
8.
When the run is complete, the Run Complete screen will be displayed (see
Figure 13).
If the method used is a custom method that has not yet been saved, the
Run
Complete screen will display a Save as option. If the method is a saved or
pre-programmed method, the Save as option will not be present. This screen
also displays an option to navigate to the Home screen or to repeat the method.
Constant current
Discontinuous buffer systems and, to a lesser extent, continuous systems increase
resistance during the run. With the current limiting setting on the PowerEase
™
Touch
350W Power Supply, the voltage will increase as resistance increases to satisfy
Ohm’s law (V=IR). If no voltage limit is set and a local fault condition occurs, such as
a poor connection, very high local resistance may cause the voltage to increase to
the maximum capacity of the power supply. This may lead to local overheating and
damage to the electrophoresis cell or create unsafe conditions.
Note: When operating under constant current conditions, it is recommended to set a
voltage limit on the power supply slightly above the maximum expected for the run.
Constant Power
Power is a function of voltage and current P=IV. For a typical gel run at constant
power, the current will decrease during the run, causing the voltage to increase. The
power limiting function may be used when running sequencing gels to remove the
ammonium persulfate from the wells and to heat the gel to an optimal temperature for
DNA separations.
Custom methods
Select Custom Methods on the Home screen to bring up the Custom Methods
screen. From here, the Saved Methods or Method Type screen can be selected (see
Figure 6).
The Saved Methods screen displays methods that have been previously saved
(shown in alphabetical order). Selecting a method brings up the run screen for the
method, allowing the method to be run or edited. The saved method can be edited
by touching any of the run parameter fields.
The Method Type screen displays method types that can be entered (see Figure 5).
Chapter 2
Methods
Custom methods
2
PowerEase ™ Touch 350W Power Supply User Guide
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