Thermo Scientific HyPerforma 2.1 S.U.B., Руководство по интеграции оборудования

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Thermo Scientific Single-Use Systems Integrator’s Guide | 13
Chapter 1 | Configuration Guidelines
Please Note: The impeller power number assumption of Np=2.1
is only an approximation, based on an academic exercise.
Recommended best practice for determination and comparison of
Np between bioreactors should be carried out by the end user and
should be based on conditions matching the specific application. End
users should also anticipate and accept some variability that may be
inherent in the analysis technique or modeling method chosen. If a
different or alternative Np value is determined by the end user, the
safety interlocks must be based solely on the maximum prescribed
RPM for a working volume as found in the reference tables in this
publication.
Never rely strictly on an estimated magnitude of
P/V when setting the agitator speed interlock levels.
Table 1.3. Maximum agitation rates (in RPM) for S.U.B.s at a P/V
of 40 W/m
3
. Using the 2,000 L S.U.B. above 20Wm
3
requires con-
sultation with Thermo Fisher Engineering.
50 L
S.U.B.
100 L
S.U.B.
250 L
S.U.B.
500 L
S.U.B.
1,000 L
S.U.B.
2,000 L
S.U.B.
Fill %
2.1 and 5.1 Systems
100 230 184 148 127 109 *
95 227 181 145 125 105 *
90 222 178 143 123 103 *
85 218 174 140 121 101 *
80 214 171 137 118 99 *
75 209 167 134 116 97 *
70 205 164 131 113 95 *
65 200 160 128 111 92 *
60 194 155 125 108 90 *
55 189 151 121 105 87 *
50 183 146 117 101 86 *
5.1 Systems Only
45 177 141 113 98 82 *
40 170 136 109 94 79 *
35 162 130 104 90 75 *
30 154 123 99 85 71 *
25 145 116 93 80 67 *
20** 135 107 86 75 64 *
* Consultation with Thermo Fisher Scientific engineers is required when operating 2,000 L
S.U.B.s at a P/V of 40 W/m
3
.
** RPM values assume the system is operating at no less than 20% volume. In order to
ensure proper volume measurements, end users are responsible for ensuring proper load
cell calibration prior to system use.
NOTE: Load cell values must be used with interlocks to keep the system operating
within recommended P/V ranges.