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Connecting the EASY-Spray Column Plumbing

Examples of LC Connections to the EASY-Spray Column

Thermo Scientific

EASY-Spray Series Ion Source User Guide

Configuring the EASY-nLC 1200 Plumbing for Two Columns

Use the touch screen on the EASY-nLC 1200 to configure the use of two columns.



To configure the EASY-nLC 1200 for use with two columns

1. On the LC touch screen, press

Maintenance > Devices

2. In the Devices list, press

EASY-nLC (HPLC)

3. Press

Properties

, clear the

One Column Setup

check box, and then press

Apply

The Home page shows the two-column setup (

Figure 17

). For additional instructions,

refer to the LC instrument manual.

Figure 17.

EASY-nLC 1200 touch screen showing a two-column configuration

Connecting an RSLCnano System to the EASY-Spray Column

Follow the appropriate procedure for a one- or two-column setup for an RSLCnano system.
The two-column setup includes a precolumn connected across the autosampler valve.

•

To plumb a one-column setup for the RSLCnano instrument

•

To plumb a two-column setup for the RSLCnano instrument

Two columns

Note

To connect the RSLCnano system to the EASY-Spray column, you must use the

Thermo Scientific Dionex UltiMate 3000 RSLCnano EASY-Spray Application Kit
(P/N 6720.0395).

Содержание EASY-Spray NG

Страница 1: ...EASY Spray Series Ion Source User Guide 60053 97275 Revision B June 2018 ...

Страница 2: ... Sciex Pte Ltd All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Therm...

Страница 3: ...e you purchased the product Number of product pieces and the estimated total weight and volume Pick up address and contact person include contact information Appropriate pick up time Declaration of decontamination stating that all hazardous fluids or material have been removed from the product For additional information about the Restriction on Hazardous Substances RoHS Directive for the European ...

Страница 4: ......

Страница 5: ...5 Controller Power Supply 6 Chapter 2 Installing the EASY Spray Series Ion Source 7 Connecting Power to the Ion Source 7 Powering the Ion Source for an EASY nLC 1200 MS System 8 Powering the Ion Source for Other nanoLC MS Systems 9 Attaching the Ion Source 9 Adjusting the Emitter Tip Position 12 Removing the EASY Spray Series Source 14 Chapter 3 Installing the Camera and LCD Monitor 15 Installing ...

Страница 6: ...ASY Spray Column 27 Additional Resources 30 Chapter 5 Configuring the Mass Spectrometer for NSI Mode 31 Configuring the EASY Spray NG Source s NSI Parameters 31 Configuring the EASY Spray Source s NSI Parameters 32 Chapter 6 Maintenance 33 Guidelines 33 Tools and Supplies 34 Cleaning the EASY Spray Column Emitter Tip 35 Replacing the Temperature Controller PCB 36 Chapter 7 Troubleshooting 39 Chapt...

Страница 7: ... column 20 Figure 12 nanoViper fitting 22 Figure 13 Recommended plumbing locations on the venting Tee nanoViper example 24 Figure 14 Tee holder secured to the bottom of the column holder 25 Figure 15 Example of a one column setup with nanoViper fittings 25 Figure 16 Example of a two column setup with nanoViper fittings and a Viper union 26 Figure 17 EASY nLC 1200 touch screen showing a two column ...

Страница 8: ...Figures viii EASY Spray Series Ion Source User Guide Thermo Scientific ...

Страница 9: ...ced on the mass spectrometer MS Also because this guide uses drawings of various connections and component parts to help illustrate procedures be sure to start from no matter where it appears Contents Accessing Documentation Providing Documentation Feedback Compatible Mass Spectrometers Special Notices Symbols and Cautions Safety Precautions Regulatory Compliance Contacting Us Accessing Documentat...

Страница 10: ...nline User Documentation Visit the Thermo Fisher Scientific website for product manuals and more To view user documentation from the Thermo Fisher Scientific website 1 Go to thermofisher com 2 Point to Services Support and click Manuals on the left 3 In the Refine Your Search box search by the product name 4 From the results list click the title to open the document in your web browser save it or ...

Страница 11: ...r complete definitions see Table 2 CAUTION If you connect the EASY Spray Series ion source to another type of MS you might impair the safety protection provided by the equipment Table 1 Ion sources and compatible mass spectrometers Ion source model Thermo Scientific mass spectrometer EASY Spray NG Orbitrap Tribrid Seriesa and TSQ Series II a In 2018 the name Orbitrap Fusion Series changed to Orbit...

Страница 12: ...spose of waste oil and when handling wetted parts of the instrument Hot surface Allow any heated components to cool before touching them Risk of electric shock This ion source uses voltages that can cause electric shock and personal injury Before removing the ion source for servicing shut down the MS and disconnect it from line power Risk of eye injury Eye injury can occur from splattered chemical...

Страница 13: ...ng components options or peripherals that Thermo Fisher Scientific has not qualified and authorized Unauthorized changes can also result in bodily injury and or damage to your system and laboratory Ensure continued compliance with regulatory standards Follow all installation instructions provided in the documentation that comes with your system Order replacement parts as specified in the instrumen...

Страница 14: ...find global contact information or customize your request 1 Go to thermofisher com 2 Click Contact Us select the country and then select the type of support you need 3 At the prompt type the product name 4 Use the phone number or complete the online form To find product support knowledge bases and resources Go to thermofisher com us en home technical resources To find product information Go to the...

Страница 15: ... of the EASY Spray Series are as follows Simple and robust design for nano liquid chromatography nanoLC MS integration Better separation with zero dead volume ZDV connections Increased productivity with the temperature controlled EASY Spray column Contents Advantages of Nanoelectrospray Functional Description Figure 1 nanoLC MS system example using the EASY Spray ion source camera and monitor Ther...

Страница 16: ...tion and droplet shrinkage often requires a drying gas thermal heating or both due to the high volume of liquid that exits the emitter Nanospray ionization NSI also known as nanoelectrospray ionization nanoESI or NSI is a form of ESI that employs low flow rates of 10 to 1000 nL min NSI or nanoESI generally does not require a drying gas or thermal heating Compared with ESI NSI tolerates a wider ran...

Страница 17: ... of the tool and then later of the emitter connect the video camera and LCD monitor if applicable For instructions see Chapter 3 Installing the Camera and LCD Monitor The column holder is mounted onto the front observation window and includes a position control knob Use the control knob to align the z axis front to back of the emitter positioning tool page 12 with the MS ion transfer tube To align...

Страница 18: ... Description 4 EASY Spray Series Ion Source User Guide Thermo Scientific Figure 3 EASY Spray ion source P N ES081 with the USB camera Source housing Column holder Camera observation window Locking lever Front observation window ...

Страница 19: ...to the heater socket which is located on the front labeled Heater The temperature control knob has an OFF setting and temperature settings in 5 C increments from 30 through 60 C As the column heats up the actual temperature appears on the display Tip If you have the Thermo Scientific EASY nLC 1200 instrument and the EASY Spray P Bus Temp Control Cablea P N 70005 63055 you can power the EASY Spray ...

Страница 20: ...mounted power supply with a detachable input plug and a power adapter cable The other end of the power adapter cable connects to the input power socket labeled P BUS located on the bottom of the ion source to provide the 24 Vdc input for the temperature controller PCB Figure 5 Controller power supply assembly shown with a European input plug Note The total length of the output cord and power adapt...

Страница 21: ...he nanoLC MS system Contents Connecting Power to the Ion Source Attaching the Ion Source Adjusting the Emitter Tip Position Removing the EASY Spray Series Source Connecting Power to the Ion Source This section describes how to connect power to the EASY Spray Series ion source when using either the EASY nLC 1200 instrument or another LC instrument such as the Thermo Scientific Dionex UltiMate 3000 ...

Страница 22: ...nnector to the P BUS socket located on the bottom of the ion source as follows a Align the red dot on the plug with the red dot on the right side of the socket Figure 6 b Push the plug into the socket Figure 6 Power connection for the temperature controller PCB bottom view 2 Connect the other end of the cable to the P BUS socket on the back of the EASY nLC 1200 instrument To add the EASY Spray ion...

Страница 23: ... plug with the red dot on the right side of the socket Figure 6 b Push the plug into the socket 4 Plug the power supply into an electrical wall outlet Attaching the Ion Source Follow these procedures to install the EASY Spray Series ion source To install the ion source onto the MS To install the optional Tee holder under the column holder CAUTION To avoid an electric shock always use the detachabl...

Страница 24: ...ere with or prevent installation of the ion source To avoid contaminating the ion transfer tube do not touch its exposed entrance Note You might need to use a small slotted screwdriver to loosen the screws on the ion sweep cone The spray cone is directly behind the ion sweep cone Align the source mounting holes with the MS pins and carefully attach the source Then lock the source levers Unlock bot...

Страница 25: ... unlock and bend the Tee holder upward as shown in the preceding image Remove the hex screw located on the bottom of the column holder Hold the Tee holder under the column holder with one hand With the other hand insert and tighten the hex bolt through the bottom of the Tee holder and the column holder The bolt is provided with the Tee holder 2 1 Bend the top part of the Tee holder down to the ext...

Страница 26: ...older Figure 8 shows the emitter positioning tool touching the MS ion transfer tube 0 mm gap The length of the tool s tip ensures the 3 mm gap for the EASY Spray column s emitter Figure 7 Emitter positioning tool Figure 8 Gap positions in front of the MS ion transfer tube side view 0 mm gap between the ion transfer tube and the emitter positioning tool 3 mm gap between the ion transfer tube and an...

Страница 27: ...hes the ion transfer tube when you adjust its position or you will damage the actual emitter when fully inserted requiring you to replace the EASY Spray column CAUTION High voltage To prevent electric shock never insert a foreign object especially a conductive part into the ion source opening The opening is intended only for the emitter positioning tool and the EASY Spray column Y Z X Slowly inser...

Страница 28: ... Cable from the ion source Unplug the controller power supply from the electrical wall outlet and then disconnect the output cable from the ion source 8 After the system has cooled to room temperature unlock the source s locking levers 9 Grasp the source housing with both hands and slowly pull it away from the MS The MS automatically switches to off mode after a few seconds You can store the sourc...

Страница 29: ...low the applicable procedure in Adjusting the Video Picture Contents Installing the USB Camera Installing the Analog Camera and LCD Monitor Adjusting the Video Picture Table 3 Camera types and installation procedures Camera P N Monitor Procedurea a Might require a 1 5 mm hex key to complete Dino Lite USB ES218 No To install the USB camera Dino Lite analog ES216 Yesb b LCD monitor P N ES217 To inst...

Страница 30: ...ation onto the data system computer 2 With the focusing wheel facing toward you slowly press the camera into the camera opening Figure 9 Figure 9 USB camera installed on the EASY Spray ion source 3 Optional Connect the camera to the USB extension cord 4 Plug the USB cord into the data system computer s USB port IMPORTANT You must install this application before connecting the camera cable to the d...

Страница 31: ... edge of the instrument 2 Install the camera as follows a Using a 1 5 mm hex key loosen the hex screw near the camera opening and then insert the camera with its focusing wheel facing toward you Figure 10 b Tighten the hex screw until it touches the camera do not overtighten it Figure 10 Analog camera installed on the EASY Spray ion source c Connect the yellow video connector to the monitor s Vide...

Страница 32: ...the video image as needed ii If the picture is too dark turn on the camera s light iii If the picture is out of focus adjust the camera s focusing wheel For the LCD monitor do the following i Turn on the monitor by pressing its POWER button ii If the picture is too dark turn on the camera s light iii If the picture is out of focus adjust the camera s focusing wheel iv If there is no picture press ...

Страница 33: ...of LC Connections to the EASY Spray Column Additional Resources Tools and Supplies Table 4 lists the required tools and supplies for configuring the LC plumbing and EASY Spray column connections Not all parts are required Note The kit for the EASY Spray Series ion source supplies the venting Tee fittings and sleeves only You are responsible for supplying the remaining parts and tools Table 4 Tools...

Страница 34: ... 0009 large size Two column setup Any nanoViper equipped precolumn This image shows the Acclaim PepMap100 Nano Trap For the PepMap100 Nano Trap Fisher Scientific 164705 Viper union for use with the EASY nLC 1200 SC904 UltiMate 3000 RSLCnano EASY Spray Application Kit Thermo Fisher Scientific 6720 0395 a Multiple sizes are available Table 4 Tools and supplies Sheet 2 of 2 Image Description Part num...

Страница 35: ...mples of LC Connections to the EASY Spray Column Setting the Column Temperature Before you begin turn the temperature control knob to the lowest temperature setting To set the temperature for the EASY Spray column Do one of the following Manually turn the ion source s temperature control knob EASY nLC 1200 instrument Use either the Thermo Xcalibur Instrument Setup window or use the EASY nLC 1200 i...

Страница 36: ...ressures up to 1200 bar 17 400 psi they are fingertight fittings that require only very small torques to seal Therefore you must follow the next procedure to avoid damage by overtightening Figure 12 nanoViper fitting IMPORTANT If you later manually turn the temperature control knob you must repeat this procedure to reset the touch screen value Table 5 Readings for the actual temperature display Di...

Страница 37: ...grees from where you felt the initial resistance Examples of LC Connections to the EASY Spray Column This section describes how to connect the one or two plumbing lines from a Thermo Scientific nanoLC instrument to the EASY Spray column in a one or two column configuration Table 4 lists the required parts Follow the appropriate topic Connecting the EASY nLC 1200 to the EASY Spray Column Configurin...

Страница 38: ...tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn Do not tighten the fitting by more than a 1 4 turn Figure 13 Recommended plumbing locations on the venting Tee nanoViper example 2 On the Waste In line insert the fitting into the venting Tee s bottom port tighten the fitting by hand until you feel resistance and then tighten t...

Страница 39: ...e the Tee holder forward Figure 14 Figure 14 Tee holder secured to the bottom of the column holder 4 Connect the venting Tee to the EASY Spray column Figure 15 Figure 15 Example of a one column setup with nanoViper fittings 5 Adjust the Tee holder s position so that it supports the nanoViper fitting and then use a 3 mm hex key to tighten the hex bolt on the bottom of the column holder Hex bolt for...

Страница 40: ...tighten 1 8 to 1 4 turn 45 90 degrees 2 On the Waste In line insert the fitting into the venting Tee s bottom port tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn Do not tighten the fitting by more than a 1 4 turn 3 Insert a 3 mm hex key into the bolt that secures the venting Tee holder to the column holder loosen the hex bo...

Страница 41: ...ess Apply The Home page shows the two column setup Figure 17 For additional instructions refer to the LC instrument manual Figure 17 EASY nLC 1200 touch screen showing a two column configuration Connecting an RSLCnano System to the EASY Spray Column Follow the appropriate procedure for a one or two column setup for an RSLCnano system The two column setup includes a precolumn connected across the a...

Страница 42: ...gure 18 2 Insert the fitting on the other end of the capillary into the Viper union tighten the fitting by hand 3 Connect the union to the EASY Spray column to complete the one column setup Figure 18 Figure 18 Example of a one column setup for an RSLCnano system direct injection CAUTION Overtightening the fittings can irreparably damage their sealing surfaces For additional information about nanoV...

Страница 43: ...t 4 on the same switching valve 3 Insert the fitting on the other end of the capillary into the Viper union tighten the fitting by hand 4 Connect the union to the EASY Spray column to complete the two column setup Figure 19 Figure 19 Example of a two column setup for an RSLCnano system preconcentration CAUTION Overtightening the fittings can irreparably damage their sealing surfaces For additional...

Страница 44: ...uring Thermo Scientific nanoLC sources log in free to planetorbitrap com choose Library Scientific Library Keyword and search for A1969 Refer to the Thermo Scientific EASY nLC Series documentation for the following Setting up the column assembly Modifying the LC instrument before connecting it to the EASY Spray Series ion source For other LC instruments refer to their documentation ...

Страница 45: ...o Tune application to configure the NSI source parameters For additional information refer to the Tune Help To set the NSI source parameters 1 Open the Tune application by choosing Start All Apps Windows 10 or All Programs Windows 7 Thermo Instruments or Thermo Model and so on 2 Click the Ion Source tab and then type 1900 in the Pos Ion Spray Voltage V box Figure 20 Use 1900 V as the start value f...

Страница 46: ...For additional information refer to the Tune Plus Help To set the NSI source parameters 1 Open the Tune Plus application by choosing Start All Apps Windows 10 or All Programs Windows 7 Thermo Instruments or Thermo Model and so on 2 Choose Setup NSI Source and then enter 1 90 in the Spray Voltage kV box Figure 21 Use 1 90 kV as the start value for the spray voltage If the intensity of the full scan...

Страница 47: ...Tip Replacing the Temperature Controller PCB Guidelines For optimal results follow these guidelines when performing the procedure in this chapter Always wear a new pair of lint and powder free gloves when handling solvents Never reuse gloves after you remove them because the surface contaminants on them recontaminate clean parts Always place the components on a clean lint free surface Have nearby ...

Страница 48: ... first aid for accidental exposure and procedures to remedy spills or leaks Read the MSDS or SDS for each chemical you use Store and handle all chemicals in accordance with standard safety procedures Always wear protective gloves and safety glasses when you use solvents or corrosives Also contain waste streams use proper ventilation and dispose of all laboratory reagents according to the direction...

Страница 49: ...y pull back on the wash cap to expose the emitter tip 4 With the other hand gently wash the emitter tip with a stream of organic solvent 5 Reinstall the column in the ion source and then use the video camera to inspect the emitter tip for any residual lint or particulates 6 If the emitter tip is not fully clean use the sonicator as follows a With the column inserted into the wash cap place the emi...

Страница 50: ...er PCB P N ES233 in the EASY Spray source remove the additional cover plate Figure 23 Figure 23 EASY Spray ion source bottom with the new temperature controller PCB and extra cover plate 3 Slowly pull out the enclosure for the temperature controller PCB Figure 24 Figure 24 Temperature controller PCB P N ES233 CAUTION High voltage To prevent electric shock make sure that you disconnect the P Bus ca...

Страница 51: ...e controller PCB 6 EASY Spray only Align the screw holes on the additional cover plate over the PCB s back two screw holes Figure 24 7 Using a 2 5 mm hex key install the four hex screws into the bottom of the ion source You can now install the EASY Spray Series ion source back onto the MS To dispose of the old PCB see WEEE Directive 2012 19 EU CAUTION To prevent damage to the electronic components...

Страница 52: ...6 Maintenance Replacing the Temperature Controller PCB 38 EASY Spray Series Ion Source User Guide Thermo Scientific ...

Страница 53: ...s too dark The camera light is off Turn on the camera light Figure 9 Column heater issues The column is not heated or The actual temperature display is blank The heater is off or there might be a loose connection or a damaged PCB Try these solutions EASY nLC 1200 Using the touch screen remove the EASY Spray from the devices list and then reenter it See To add the EASY Spray ion source to the EASY ...

Страница 54: ...pray to spit Try degassing the mobile phase or purging the line and then recheck the line for air bubbles There is an emitter blockage from particles in the sample other small particles from the flow lines or valves and so on Try adjusting the spray voltage If that does not resolve the blockage problem either clean the emitter see Cleaning the EASY Spray Column Emitter Tip or insert a new EASY Spr...

Страница 55: ...ose View Display Status View and then click the All tab 2 Choose Setup NSI Source 3 Compare the setting in the NSI Source dialog box to the readback value for the NSI source s absolute spray voltage A green check mark indicates that the parameter is functioning properly The ion source s HV connector has a loose connection If you have a digital multimeter that can measure 20 M mega ohms do the foll...

Страница 56: ...7 Troubleshooting 42 EASY Spray Series Ion Source User Guide Thermo Scientific ...

Страница 57: ...emp Control Cable for the EASY nLC 1200 instrument LC167 EASY Spray tools emitter positioning tool and 3 mm hex key ES232 LCD monitor and power supply ES217 Tee holder ES234 USB extension cord 00302 99 00095 Wash cap for the EASY Spray column ES235 Consumables Peptide Columns EASY Spray column 15 cm 75 μm ID PepMap C18 3 μm particles 100 Å pore size ES800 EASY Spray column 15 cm 50 μm ID PepMap RS...

Страница 58: ... Columns EASY Spray column 25 cm 200 μm ID PepSwift Monolith ES810 EASY Spray column 15 cm 75 μm ID Accucore C4 2 6 μm particles 150 Å pore size ES811 EASY Spray column 15 cm 75 μm ID PepMap C18 3 μm particles 300 Å pore size ES812 For other consumables visit www thermofisher com easyspray www fishersci com and www unitylabservices com Transfer Lines EASY Spray transfer line 7 μm ID emitter 20 μm ...

Страница 59: ...ts of the EASY Spray Series ion source kits Sheet 1 of 2 Image Description Quantity Part number Provided in the EASY Spray NG Ion Source Kit P N ES082 See Figure 2 EASY Spray NG housing 1 a Provided in the EASY Spray Ion Source Kit P N ES081 See Figure 3 EASY Spray housing 1 b Provided in both ion source kits Controller power supply assembly Power supply 24 Vdc output shown without the input plug ...

Страница 60: ... F 233 Wash cap for the EASY Spray column 1 ES235 a The EASY Spray NG source housing comes with the EASY Spray NG Ion Source Kit P N ES082 and cannot be purchased separately b The EASY Spray source housing comes with the EASY Spray Ion Source Kit P N ES081 and cannot be purchased separately c The ion source kit includes either the analog camera P N ES216 with the LCD monitor P N ES217 or the USB c...

Страница 61: ...20 troubleshooting 39 EASY Spray P Bus Temp Control Cable 5 electrical wall outlets number of 7 emitter positioning tool 12 See also procedures F figures list of vii H heater cable 20 troubleshooting 39 high voltage internal cable location 37 troubleshooting 41 I ion sources compatible mass spectrometers xi housing description 3 installing 10 locking levers positions 3 photo of 3 4 power connectio...

Страница 62: ...tor installing 15 nanoViper fittings using 23 video picture adjusting 18 R regulatory compliance xiii replaceable parts 43 S safety symbol descriptions xi signal intensity troubleshooting 40 source See ion source spray troubleshooting 40 voltage readback value 41 setting the value EASY Spray 32 setting the value EASY Spray NG 31 symbols meaning xi T Tee holder adjusting position 25 shipping config...

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