Thermo Scientific Applied Biosystems QuantStudio 6 Pro, Руководство пользователя

Страница: 109 / 241

The instrument and block require valid ROI/uniformity, background, normalization, and dye calibrations
to perform verification.
For a description of verification, see “Instrument verification description” on page 104 .
For a description of the pass criteria, see “Performance specifications pass criteria” on page 105 .
RNase P instrument verification TaqMan ™ Array Card
The ViiA
™
7 Array Card RNaseP Verification Kit includes one empty array card and eight tubes of
solution. Each tube contains reaction mix (PCR master mix, RNase P primers, and FAM
™
-MGB dye-
labeled probe) and a known concentration of human genomic DNA template.
To perform an instrument verification run, each solution is loaded into the empty array card. When
complete, the array card contains five replicate groups of standards (200, 400, 800, 1600, and
3200 copies), two of unknown populations (800 and 1600 copies), and one that serves as a no template
control (NTC).
See Figure 15 on page 109 .
1
2
3
4
5
6
7
8
Figure 15 RNase P standards layout
1
Port 1, No Template Control
2
Port 2, Unknown A (800 copies)
3
Port 3, Unknown B (1,600 copies)
4
Port 4, Standard 200 copies
5
Port 5, Standard 400 copies
6
Port 6, Standard 800 copies
7
Port 7, Standard 1,600 copies
8
Port 8, Standard 3,200 copies
Chapter 7
Calibrate and verify instrument performance
Perform verification using RNase P with a TaqMan ™ Array Card
7
QuantStudio ™ 6 Pro Real-Time PCR System and QuantStudio ™ 7 Pro Real-Time PCR System User Guide
109