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vii

BioOptix™ 10 

Table of Contents

Section 1 Introduction

1.1 Product Overview  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1-1
1.2 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1-2
1.3 System Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1-3

1.3.1 BioOptix 10 Front Panel Controls   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1-3
1.3.2 BioOptix 10 Left-side Controls and Connections  . . . . . . . . . . . . . . . . . . . . .  1-4
1.3.3 BioOptix 10 Right-side Connections  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1-5
1.3.4 BioOptix 10 Rear Panel Controls and Connections  . . . . . . . . . . . . . . . . . . .  1-6

Section 2 Installation

2.1 Unpacking the BioOptix 10 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-1
2.2 BioOptix 10 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-1

2.2.1 BioOptix 10 Location and Space Requirements . . . . . . . . . . . . . . . . . . . . . .  2-1
2.2.2 Electrical Requirements   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-2
2.2.3 Assembling the BioOptix 10  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-2
2.2.4 Assembling the Foxy 200 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-9
2.2.5 Fluid Connections   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-10
2.2.6 Windows PC Connection  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-14

2.3 BioOptix 10 Software Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-14

2.3.1 Configuring BioOptix 10 Software  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-14

2.4 BioOptix 10 System Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2-15

Section 3 Operation

3.1 Sample Preparation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-1
3.2 Column Installation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-1

3.2.1 Column Tubing and Adapters  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-1

3.3 BioOptix 10 Software  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-2

3.3.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-2

3.4 Gradients . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

3.4.1 Defining a Gradient  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-3
3.4.2 Saving the Gradient   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-3

3.5 Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

3.5.1 Equilibrate Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-4
3.5.2 Load Settings  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-5
3.5.3 Wash Settings   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-6
3.5.4 Elute Settings   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-6
3.5.5 Flush Settings   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-7
3.5.6 Saving the Method File  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-7

3.6 Experiments  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-7

3.6.1 Setting up the Experiment   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-8
3.6.2 Editing the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-8
3.6.3 Saving the Experiment File   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-9

3.7 Running the Experiment  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-9

3.7.1 System Operation   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-9
3.7.2 Operation Displays   . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-10
3.7.3 Suspending System Operation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  3-10

Содержание 10

Страница 1: ...BioOptix 10 Installation and Operation Guide 69 5203 150 Copyright 2004 Teledyne Isco Inc All rights reserved Revision B September 20 2005 ...

Страница 2: ......

Страница 3: ...Return Authorization Number specified Be sure to include a note describing the malfunction This will aid in the prompt repair and return of the equipment Teledyne Isco welcomes suggestions that would improve the information presented in this manual or enhance the operation of the equipment itself Teledyne Isco is continually improving its products and reserves the right to change product specifica...

Страница 4: ......

Страница 5: ...s il est très recommandé que vous le faites à l intérieur d un environnement isolé conçu pour tels liquides Cet environnement isolé devrait être construit selon les règlements fédéraux provinciaux et locaux aussi que le plan de votre compagnie qui concerne l évènement d un accident avec les matières hasardeuses WARNING Avoid hazardous practices If you use this instrument in any way not specified i...

Страница 6: ...En tout cas utilisez toujours de pratiques bonnes de la laboratoire et des procédures standardes de la sûreté Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts Three levels of severity are described in the sample alerts below CAUTION Cautions identify a potential hazard which if not avoided may result in minor or moderate injury This category can also warn you ...

Страница 7: ...écurité Ce symbole signale l existence d instructions importantes relatives au pro duit dans ce manuel Ce symbole signale la présence d un danger d électocution Warnungen und Vorsichtshinweise Das Ausrufezeichen in Dreieck ist ein Warnzeichen das Sie darauf aufmerksam macht daß wichtige Anleitungen zu diesem Handbuch gehören Der gepfeilte Blitz im Dreieck ist ein Warnzeichen das Sei vor gefährlich...

Страница 8: ...BioOptix 10 Safety vi ...

Страница 9: ... 2 10 2 2 6 Windows PC Connection 2 14 2 3 BioOptix 10 Software Installation 2 14 2 3 1 Configuring BioOptix 10 Software 2 14 2 4 BioOptix 10 System Preparation 2 15 Section 3 Operation 3 1 Sample Preparation 3 1 3 2 Column Installation 3 1 3 2 1 Column Tubing and Adapters 3 1 3 3 BioOptix 10 Software 3 2 3 3 1 Overview 3 2 3 4 Gradients 3 2 3 4 1 Defining a Gradient 3 3 3 4 2 Saving the Gradient ...

Страница 10: ...5 3 Electrical 4 8 4 5 4 Inaccessible Fuses 4 8 4 5 5 Inaccessible Breakers 4 8 4 6 Service Department 4 10 4 7 How to Ship Returns 4 10 Appendix A Method Templates A 1 Opening the Template A 1 A 2 Modifying the Template A 3 A 2 1 General Editing Rules A 3 A 2 2 Blocks A 4 A 2 3 Blockn A 4 A 2 4 Other A 4 Appendix B Fluid Path Diagrams B 1 Fluid type Blocks B 1 B 2 Load type Blocks B 2 B 3 Gradien...

Страница 11: ...ctions 2 13 2 17 Adhesive retainers attached 2 13 2 18 Column bypass tubing 2 16 2 19 Mode control knob 2 16 2 20 BioOptix 10 Manual Hardware Control window 2 17 3 1 Gradient File 3 2 3 2 Equilibrate tab settings 3 4 3 3 Load tab settings 3 5 3 4 Wash tab settings 3 6 3 5 Elute tab settings 3 6 3 6 Flush tab settings 3 7 3 7 Experiment File 3 8 3 8 BioOptix 10 Operation Buttons 3 9 3 9 Pause at en...

Страница 12: ...ptix 10 Front Panel Controls 1 3 1 3 BioOptix 10 Left side Connections 1 4 1 4 BioOptix 10 Right side Connections 1 5 1 5 BioOptix 10 Rear Panel Controls and Connections 1 6 2 1 Tubing Color chart 2 12 2 2 Windows PC Minimum Requirements 2 14 3 1 Operation Buttons 3 9 4 1 Common Problems 4 7 ...

Страница 13: ...ctions of interest System highlights include Individual detector on each channel Unique gradient conditions can be used for each channel great for scouting ideal gradient conditions Repeatable uniform flow for each sample regardless of buffer gradient or sample consistency Ten independent precision pumps provide flow rates from 0 5 to 12 mL min for each channel Fast and easy sample loading individ...

Страница 14: ...g water Gradient Accuracy 5 full scale using water Gradient Linearity 5 full scale using water Operating Pressure 0 to 6 895 bar 0 to 100 psi Flow Cell Path Length 2 mm Detector Wavelength 190 to 360 nm Detector Noise 5 10 4 AU Detector Drift 1 10 3 AU hr CE Conformity Specifications a d Pollution Degree 2 Installation Category II Maximum Altitude 2000 meters a Excludes Foxy 200 Fraction Collector...

Страница 15: ...ion port for syringe injected liquid samples 2 Mode Knob Configures fluid path for purging manual sample injection or purification operation 3 Column Inlet Port Allows connection of tubing to column inlet 4 Column Clamp Position Adjust Secures column clamp and allows for horizontal and rotational positioning of columns 5 Column Outlet Port Allows connection of tubing from column outlet 6 Column Cl...

Страница 16: ...s to accept sample for each channel 4 Pump Purge Port When the Mode Knob is in the Purge Pump position pumped fluids are diverted to this port bypassing the column detection and collection 5 Waste Ports Connects to waste collection container s Connecting to separate contain ers allows isolation of waste buffers by each channel which could be benefi cial should a purified sample fail to collect 6 I...

Страница 17: ... BioOptix 10 Right side Connections Figure 1 3 BioOptix 10 right side connections 1 Table 1 4 BioOptix 10 Right side Connections Item Name Description 1 Purified Sample Outlet Channels 1 through 10 which connect to the Foxy 200 Fraction Collector ...

Страница 18: ...e type fuses 100 117 VAC 3 amp T type fuse 234 VAC 1 5 amp T type fuses Internal fuses are not accessible to the user and should only be replaced by a trained service technician Pump Drive Board F101 4 0 amp T type fuse Power One PS F1 Not Serviceable Lamp Drive Board F1 375 amp T type fuse Case Bottom Assy F4 Not Serviceable T2 primary F5 Not Serviceable T2 secondary F6 Not Serviceable T2 seconda...

Страница 19: ...Isco or its authorized representative for advice Compare the contents of the boxes with the enclosed packing slip If there are any shortages contact Teledyne Isco immediately 2 2 BioOptix 10 Installation 2 2 1 BioOptix 10 Location and Space Requirements The system should be installed on a work surface capable of sup porting the system and buffer sample and waste containers The system is designed f...

Страница 20: ...f the BioOptix 10 is factory set Refer to the serial number label on the right side panel to determine the electrical requirements Teledyne Isco recommends that the BioOptix 10 system runs on a dedicated AC circuit to prevent abnormal conditions that may be caused by high current or electrical noise The BioOptix 10 and Foxy 200 should be connected to the same circuit to prevent ground loop anomali...

Страница 21: ...lete the connections 1 Release the two sets of tubes from the retaining clips Fig ure 2 3 2 Remove the four screws securing the back metal plate on the column module Figure 2 3 Then lift and remove the plate Figure 2 3 Removing the column module back plate Pump Module Column Module Screws Screws Retaining Clips ...

Страница 22: ...nel Route these two bundles through the slots at the edge of the panel Figure 2 4 Figure 2 4 Routing the Sample Out lines column module 4 Connect the beige 25 pin connector from the pump module to the column module connector labeled Pump Controller Refer to Figure 2 5 Figure 2 5 Pump controller connection Route tubing through slots To Foxy 200 From Pump Module ...

Страница 23: ...nector labeled BD3 P110 to P110 on the Power Drive Circuit Board Figure 2 6 Figure 2 6 P110 Connection on BD3 6 Connect the plug labeled BD1 J106 to J106 on the Control ler Circuit Board Figure 2 7 Figure 2 7 J106 Connection on BD1 From Pump Module From Pump Module ...

Страница 24: ...s in the plate will fit around the connections made in steps 3 through 7 Secure the plate with the four screws Figure 2 3 Then reattach the tubing to the retaining clips 9 The remaining ribbon cable connections are routed from the top of the pump module through the opening on the back plate of the column module Refer to Figure 2 9 and complete the connections matching the connector labels Figure 2...

Страница 25: ... Loosen the nut on the compression fitting insert the tubing fully into the fitting and finger tighten the nut Then use a wrench to tighten the nut an additional full turn Repeat for all ten channels c Replace the splash guard so that the tubing is routed up and over the guard Figure 2 10 Liquid connections pump module shown 11 Slide the two modules together Refer to Figure 2 11 Align the eight ho...

Страница 26: ...BioOptix 10 Section 2 Installation 2 8 Figure 2 11 Aligning the fasteners Figure 2 12 Column clamps inserted ...

Страница 27: ...ecure the clamps by tighten the thumbscrews against the stain less steel shafts Figure 2 12 2 2 4 Assembling the Foxy 200 1 Refer to section 2 of the Foxy 200 X Y Fraction Collector instruction manual Unpack the Foxy 200 and assemble the arm Note To prevent damage to the ground wire on the fraction collector arm attach the ten channel drop former to the arm before pro ceeding with the arm assembly...

Страница 28: ...is kit also includes a tubing cutter Except as noted on the Foxy connections use the following steps for tubing connections 1 Loosen the nut on the compression fitting 2 Insert the tubing fully into the fitting 3 Finger tighten the nut 4 Using a wrench tighten the fitting an additional full turn Buffer A These inlet ports are located on the left side of the BioOptix 10 The ferrules on these inlet ...

Страница 29: ... the Buffer Assembly b Attach the mounting block to the two holes using the hex head screws c Attach the manifold to the mounting block with the Phillips screws and lock washers d Connect the ten Teflon tubes each 3 long by 0 085 ID from the manifold to the Buffer A inlet ports e Cut to length a section of 0 250 OD Teflon tubing P N 023 0508 01 from the accessory kit and route the buffer solution ...

Страница 30: ...n point If there might be a need to recover waste fluid e g undetected purified sample a collection container for each channel is recom mended Isolating waste fluid for each channel can simplify sample recovery Pump Purge This port is used when the pumps clears any fluid remaining in the pump This fluid is cleared before a run is started or during system maintenance Connect a length of 0 250 OD Te...

Страница 31: ...hread and finger tighten the fittings into the ten channel drop former assembly After connecting the tubing peel the adhesive backing from each retainer Then attach each retainer to the top of the Foxy 200 arm Figure 2 17 Figure 2 17 Adhesive retainers attached Blue Yellow Orange Green Clear Blue Yellow Orange Green Clear 1 2 3 4 5 6 7 8 9 10 1 10 Retainers with adhesive backing ...

Страница 32: ...file named setup exe on the CD ROM drive 4 Follow the on screen instructions to complete the software installation 2 3 1 Configuring BioOptix 10 Software After installing the BioOptix 10 software on the PC the software should be configured to match the hardware of the BioOptix 10 system To configure the software 1 Start BioOptix 10 software The software installation routine placed an icon on the d...

Страница 33: ...l value will vary according to the proper ties of the stationary and mobile phase Select a value low enough to limit the flow rate when a column or tub ing blockage may be indicated but high enough to avoid false indications when higher column operating pressures may be the norm Default Data Directory Click the Browse button to select a default data directory 4 Click the Save button to accept the ...

Страница 34: ...n Inlet and Outlet ports Figure 2 18 Figure 2 18 Column bypass tubing 7 For each channel set the mode control knobs to Purge Fig ure 2 19 Figure 2 19 Mode control knob 8 From the BioOptix 10 software menu select Tools Manual Control The BioOptix 10 Manual Hardware Control win dow is displayed Figure 2 20 Column Inlet Column Outlet ...

Страница 35: ...assing through the internal pump and valves Buffer B solution should be visible at the Pump Purge port 11 Continue to manually pump Buffer B solution through the system and inspect the tubing connections If any connections are leaking use a wrench to tighten the fitting 1 4 turn then check again for leaks Repeat as neces sary If no leaks are found proceed with step 12 12 Click the Stop Pump button...

Страница 36: ...BioOptix 10 Section 2 Installation 2 18 ...

Страница 37: ...ng holes on the side of the BioOptix 10 accept 9 5 mm 3 8 in mounting stems To install a column insert the column into the clamp and turn the knob to close the clamp and secure the column When inserting any column be sure to observe the flow direction if noted on the column Orient the column with the inlet at the top and the outlet at the bottom 3 2 1 Column Tubing and Adapters Because of the vari...

Страница 38: ...ain settings that direct the operation of all channels Experiment files are based on method file settings The BioOptix 10 system operation can be started after the experiment settings are defined Sections 3 4 through 3 6 describe these files in detail 3 4 Gradients A gradient file can be created by selecting the File New Gradient menu command pressing the Ctrl G keyboard buttons or by clicking the...

Страница 39: ...s Insert Row adds a row above the selected row Delete Row removes the selected row from the table Add Row adds a row to the end of the table Sharp changes can be made in the gradient by entering a row with 0 mL as the pumped volume followed by another row with the new B and volume Pairing rows such as these can be used to form step gradients 3 4 2 Saving the Gradient To be used in method and exper...

Страница 40: ...g the purification Figure 3 2 Equilibrate tab settings The Equilibrate tab Figure 3 2 contains the following Fluid B Enter the desired mix of buffer fluid as a percentage of Buffer B Flow Rate mL min Enter the desired flow rate from 0 5 to 12 mL min Volume mL Enter the total amount of Buffer fluid to pump during this block Collect Effluent Select the desired action for effluent fluid as it comes o...

Страница 41: ...id sample from the Sample In ports onto the column Select Manual to inject the sample from the manual injection ports located on the top of the BioOptix 10 onto the column Note that selecting Manual disables the flow rate and volume settings Flow Rate mL min If a Pump injection is selected enter the desired flow rate in mL min Volume If a Pump injection is selected enter the desired volume in mL C...

Страница 42: ...or a discussion on the settings for this tab Figure 3 4 Wash tab settings 3 5 4 Elute Settings This block pumps the buffer solutions through the column The mixture and quantity of buffer solution is defined by the gradient file Figure 3 5 Elute tab settings The Elute tab Figure 3 5 contains the following Gradient Click the Browse button to select a gradient file Volume mL This is reported by the g...

Страница 43: ...e used in an experiment file the method file must be saved Save the method by selecting the File Save menu command Use a descriptive file name so that it can be located later 3 6 Experiments An experiment file can be created by selecting the File New Experiment menu command pressing the Ctrl E keyboard buttons or by clicking the E toolbar button Before building and displaying the experiment file y...

Страница 44: ...compatible file name 2 Advance the cursor to the next cell to the right BioOptix 10 completes the remaining cells in the row using the settings from the method file as defaults 3 Repeat steps 1 and 2 for all channel positions that will be in use The table now is populated with default settings for each channel position in use Figure 3 7 Experiment File 3 6 2 EditingtheExperiment The table can be e...

Страница 45: ... 9 Similarly the BioOptix 10 would proceed through the Sample Load Wash Elute and Flush blocks Stop Next Tube Play Fast Forward Table 3 1 Operation Buttons Button Description Play Click to begin the experiment run If the Stop button was clicked during the run clicking the Play button will resume the run Stop Click to suspend the run activity From this state you click Play to resume the run or clic...

Страница 46: ...ging across part of the plot area will zoom in on the selected region In Pan mode clicking and dragging moves the plot area under the cursor to a new position The view can be returned to the default view by clicking the Reset Graph button Absorbance trace In addition to the zoom feature which scales the plot area both horizontally and vertically the scale of the absorbance trace can be modified al...

Страница 47: ...s a wash fluid through the pumps to prevent salts from crys tallizing in the system CAUTION Salts will cause premature wear to the internal components and eventually pump failure Figure 3 10 Pump flush option If another experiment will be started immediately this flush can be skipped Otherwise if the system will remain idle for a few hours or even overnight the pump flush is recommended Flush the ...

Страница 48: ... using BioOptix 10 software To do so select the File Open menu command or click the Open toolbar button Multiple channels from the experiment can be opened by select the lst file Indi vidual channels can be viewed by opening a run file Viewing the run data and chromatograms is very similar to the operation displays described in section 3 7 2 The run data and chromatograms displayed in the active w...

Страница 49: ...ter the run the BioOptix 10 can automatically print the purifi cation run results or save the file in a format listed in section 3 8 2 To automatically receive the hard copies or save the files select the Tools AutoReport menu command and modify the set tings ...

Страница 50: ...BioOptix 10 Section 3 Operation 3 14 ...

Страница 51: ...flush the pump at the end of a purification run see section 3 8 If the system will remain idle for a few hours or even overnight a pump flush fol lowing the run is recommended The pump flush can also be initiated from a menu command Daily Pump Flush 1 Select Tools Edit Pump Flush and review the settings Save any changes you ve made 2 Ensure the Buffer In lines are placed in containers of wash flui...

Страница 52: ...l may open the case enclosure WARNING Earth ground bonding connector Do not remove or disconnect 1 Remove the rear cover item 1 Figure 4 2 by unfastening the nine screws located on the top and back of the BioOptix 10 2 Unfasten the bottom 2 screws and rotate the valve assem bly up Slide the slide lock over to catch and hold the valve assembly 3 Remove the upper valve assembly item 2 Figure 4 2 by ...

Страница 53: ...he glass cylinder 12 Remove the glass cylinder and bottom O ring This exposes the push rod and seal 13 The push rod shaft has two slots for a wrench Hold the push rod at the slots with a 3 16 wrench and remove the 6 screw and washers from the bottom of the push rod 14 Lift the push rod out of the assembly 15 Using care to avoid damaging the push rod cut the seal with a sharp blade Then pull the se...

Страница 54: ...ston Plug Top O Ring P N 202 5001 09 Glass Cylinder Seal P N 202 9090 32 Push Rod Bottom O Ring P N 202 5001 09 375 OD Flat Washer 5 OD Rubber Washer 5 OD Flat Washer 6 Interior Lock Washer 6 1 4 Pan Head Screw Retainer Plate 6 Interior Lock Washer 6 1 4 Pan Head Screw Top Mounting Tab Bottom Mounting Tab Wrench Slots Inductor 8 32 1 1 4 Pan Head Screw Push Rod Seal P N 202 9090 32 ...

Страница 55: ...asher into the bottom mounting tab c Insert and tighten the four screws with lock washer through the side of the case item 4 Figure 4 2 21 Place the drip tray item 3 Figure 4 2 back into the sys tem 22 Return the upper valve assembly item 2 Figure 4 2 to its original location and secure with the mounting hardware 23 Place the back cover item 1 Figure 4 2 on the BioOptix 10 and secure it with the m...

Страница 56: ... the waste port mount ing plate Figure 4 5 The optical assembly is located behind this plate Figure 4 5 Waste port mounting plate Figure 4 6 Deuterium lamp location 2 Disconnect the wiring connector 3 Release the twist lock cable clamp 4 Remove the three lamp mounting screws 5 Carefully pull the lamp straight out from the optical assembly Lamp Mounting Screws Twist Lock Clamp ...

Страница 57: ...ormance of the system If you notice in your log that detector responses have changed or you are running samples and the absorbance readings are not what you think they should be you should run a standard If you run a standard sample and obtain the expected results the problem is not being caused by the instrument You can then examine other causes for your problem such as the column or mobile phase...

Страница 58: ...ses WARNING Risk of electrical shock Disconnect the electrical power before opening the rear panel or otherwise gaining access inside the equipment Only trained service personnel may gain access to the interior Internal fuses are not accessible to the user and should only be replaced by a trained service technician Pump Drive Board F101 4 0 amp T type fuse Power One PS F1 Not Serviceable Lamp Driv...

Страница 59: ...the circuit breaker separate the pump and column modules see section 2 Locate the circuit breaker illustrated in Figure 4 7 and press the button Figure 4 7 Circuit breaker location The thermal breaker is located behind the waste port panel Figure 4 6 To reset the breaker open the panel and locate the reset switch Figure 4 8 Insert a small flat blade screwdriver into the opening and push the switch...

Страница 60: ...an be obtained from our Web site at http www isco com 4 7 How to Ship Returns Be sure all parts and hardware are back in place before packing Wrap the unit in heavy paper or put it in a plastic bag If the original shipping carton is not available put the wrapped unit in a strong cardboard box at least six inches longer in each basic dimension than the unit itself Fill the box equally around the un...

Страница 61: ...ile Rename the desired template file as Standard tpl when needed keeping multiple files each named as Standard tpl in separate uniquely named subfolders Copy the desired template file to the same folder as the biooptix exe file as needed A combination of these file maintenance techniques may also be practical However with any file maintenance technique care should be taken to prevent overwriting t...

Страница 62: ...ix 10 software is shown in Figure A 1 Figure A 1 Installed Standard tpl file Tip You can instruct Windows to always open a tpl file with Notepad or other text editor To do so 1 Open Windows Explorer and locate the file named Stan dard tpl 2 Highlight the file and right click to display menu options 3 Select the Open With option Windows will display a list of programs 4 Highlight Notepad or the pre...

Страница 63: ...e within the acceptable range Count 5 in Figure A 2 is an example of a numeric value Text entries Text entries are enclosed in quotes and follow an equal sign Enter text between the quotes observing the string length or option requirements Any printing ASCII character may be used Nonprinting characters such as returns line feeds tabs must be avoided Entries are not case sensitive Name Equilibrate ...

Страница 64: ...ee options which determine the controls that will appear on the tab Fluid This type provides controls for pumping an isocratic mix of buffer solution An example of a Fluid tab can be seen in Figure 3 2 Load This type provides controls to pump liquid sample onto the column or to allow a manual injection An example of a Load tab can be seen in Figure 3 3 Gradient This type is used to reference a Gra...

Страница 65: ...he pump to draw either sample from the Sample In ports or buffer solution Channel This valve controls allows fluid to be pumped only when the channel is active Mode This manually controlled front panel valve has three positions Operate passes fluid from the pump to the column Manual Inject directs injected fluid onto the column Purge Pump diverts fluid from the pump to a purge port that is common ...

Страница 66: ...tical when the fluid block is in the experiment for column equilibration Collecting the effluent may be desirable if the fluid may contain something of interest e g during a column wash Figure B 2 Inactive channel B 2 Load type Blocks Load blocks apply the sample onto the column There are two modes that can be selected in the Method file Pump or Manual injection Pump injections draw liquid sample ...

Страница 67: ...he liquid sample is intro duced directly onto the column Figure B 4 Figure B 4 Fluid path for Load type blocks Manual injections B 3 Gradient type Blocks Gradient blocks are similar to fluid type blocks Figure B 5 The Buffer valve switches between buffers A and B to produce the correct mix Figure B 5 Fluid path for Gradient type blocks Mode Effluent To Foxy Manual Sample Injection Column Buffer Sa...

Страница 68: ... will be idle for more than a few hours See sections 3 8 and 4 1 The flush removes salts from the Buffer in lines to the Mode knob Figure B 6 Figure B 6 Pump flush B 5 Plumbing Diagram A complete plumbing diagram can be found on the following page Buffer Sample Channel Mode Pump Buffer A Buffer B To Pump Purge ...

Страница 69: ...BioOptix 10 Appendix B Fluid Path Diagrams B 5 Figure B 7 BioOptix 10 Plumbing Diagram ...

Страница 70: ...BioOptix 10 Appendix B Fluid Path Diagrams B 6 ...

Страница 71: ... the call outs in the accompanying tables to determine the part number for the item Replacement parts can be purchased by contacting Teledyne Isco s Customer Service Department Teledyne Isco Inc Customer Service Department P O Box 82531 Lincoln NE 68501 USA Phone 800 228 4373 402 464 0231 FAX 402 465 3022 E mail info isco com ...

Страница 72: ...BioOptix 10 Appendix C Replacement Parts Lists C 2 ...

Страница 73: ...13BioOptix 10 Replacement Parts Lists C 3 13BioOptix 10 Replacement Parts Lists C 1 Detector Assembly ...

Страница 74: ...13BioOptix 10 Replacement Parts Lists C 4 ...

Страница 75: ...13BioOptix 10 Replacement Parts Lists C 5 ...

Страница 76: ...13BioOptix 10 Replacement Parts Lists C 6 ...

Страница 77: ...13BioOptix 10 Replacement Parts Lists C 7 C 2 Column Module ...

Страница 78: ...13BioOptix 10 Replacement Parts Lists C 8 ...

Страница 79: ...13BioOptix 10 Replacement Parts Lists C 9 ...

Страница 80: ...13BioOptix 10 Replacement Parts Lists C 10 ...

Страница 81: ...13BioOptix 10 Replacement Parts Lists C 11 C 3 Pump Module ...

Страница 82: ...13BioOptix 10 Replacement Parts Lists C 12 ...

Страница 83: ...13BioOptix 10 Replacement Parts Lists C 13 ...

Страница 84: ...13BioOptix 10 Replacement Parts Lists C 14 ...

Страница 85: ...erity Applied Performance Criteria EN61000 4 2 Electrostatic Discharge Level 2 4kV contact discharge Level 3 8kV air discharge B B EN61000 4 3 Radiated RF Immunity 80 MHz to 1000MHz 80 AM at 1kHz Level 1 1V m A EN61000 4 4 Electrical Fast Transient Level 2 1kV on ac lines B EN61000 4 5 Surge on AC Lines 1kV common mode 500V differential mode B EN61000 4 6 Conducted RF on AC lines 150 kHz to 80 MHz...

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Страница 87: ...period of one year 365 days from their shipping date or from the date of installation by an authorized Teledyne Isco Service Engineer as may be appropriate During the warranty period repairs replacements and labor shall be provided at no charge Teledyne Isco s liability is strictly limited to repair and or replacement at Teledyne Isco s sole discretion Failure of expendable items e g charts ribbon...

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