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BioOptix™ 10
Section 3 Operation
3-5
Pausing at the end of a block allows for buffer changes or
other mechanical adjustments before proceeding with the
next block.
•
Wavelength
– Type or select the desired wavelength from
190 to 360 nm to be used for UV detection. Note that all
blocks use the same wavelength.
Note
The Equilibrate tab is a “Fluid” block type. See Appendix A.
3.5.2 Load Settings
This block loads the sample onto the column.
Figure 3-3
Load tab settings
The Sample Load tab (Figure 3-3) contains the following:
•
Injection
– Select
Pump
to pump liquid sample from the
Sample In ports onto the column. Select
Manual
to inject
the sample from the manual injection ports (located on
the top of the BioOptix 10) onto the column. Note that
selecting
Manual
disables the flow rate and volume
settings.
•
Flow Rate, mL/min
– If a
Pump
injection is selected, enter
the desired flow rate in mL/min.
•
Volume
– If a
Pump
injection is selected, enter the
desired volume in mL.
•
Collect Effluent
– Select the desired action for effluent
fluid as it comes off the column. If
Yes
is selected, enter
the desired fraction size.
•
Pause at End
– Select
Yes
to have the system wait for
operator intervention after completing this block. Select
No
to automatically advance to the next block.
Note
The Load tab is a “Load” block type. See Appendix A.
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