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5. APPLICATION NOTES
Generation of micro particle free plasma samples and recovery of micro
particles from platelet poor plasma:
Ceveron
®
MFU 500 is designed to be used for filtration of fresh, clear,
platelet poor plasma samples.
For turbid plasma samples pre-filtration through another filter with larger
pores is required.
What is the efficiency for removal of micro particles ?
After filtration of plasma spiked with 10,000 MP/
µ
l only 0.4% ± 0.004% of
MPs were found in the filtrate.
Recovery of micro particles from the filter plate
MPs can be recovered from the filter plate by rinsing each well with 200µL
of PBS or micro particle free plasma. Rinsing is performed by gentle
pipetting the sample for 3 to 5 times and then pipetting off the sample.
Recovery rates are in general > 85 %
Sample Material
Sample
Pre-filtration /
filter required
Notes
PPP
NO
Collection of PPP under
standardized condition is required
PRP
YES
Instead of pre-filtration prepare
PPP by standard centrifugation
procedure
Whole Blood
YES
Prepare PPP by standard
centrifugation procedure
Lipaemic Plasma
YES
5 µm filter
Lyophilized Plasma
YES
5 µm filter
Some lyophilized plasma do not
require pre-filtration
6. REPLACING O-RING AND GASKET
An accessory kit containing an additional O-ring, gasket and Allen wrench is
included with each unit of Ceveron
®
MFU 500. O-ring and gasket should be
replaced when worn or vacuum leakage is observed.
For replacing the black gasket, the 12 screws have to be loosened by using
the Allen wrench included in the accessory kit. The O-ring can simply be
replaced manually.
7. CLEANING AND STORAGE
Basic preventive maintenance requires regularly cleaning of
Ceveron
®
MFU
500.
Use a mild soap, standard laboratory detergent, a dilute solution of sodium
hypochlorite, or ethyl alcohol to clean all surfaces of the manifold. After
cleaning, rinse off any residual liquid with a soft cloth or paper towel
dampened in clean water, then wipe dry.
Should there be any liquid leakage into the manifold base or the vacuum
tubing, it can be removed by rinsing the base with distilled water while the
vacuum pump is running (the pump is water resistant). After rinsing with
water, air should be pumped through the unit at least for 2 runs to dry out
the system
Other than occasional cleaning, the Power Supply is maintenance-free. For
cleaning the case, use a clean, lint-free, antistatic and dry cleaning cloth.
Store in a controlled environment at a temperature between 15°C and 30°C.
Содержание Ceveron MFU 500
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