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9
10
Seal the filter plate tightly with a plate sealer foil (provided).
Stop Cock
k
has to be in the open position (in line with the tubing
i
).
Press the start button
e
.
Press the filter plate
a
Note: If a turbid plasma sample
cannot be filtered completely
within the preset time, repeating the filtration will not have an effect as
the filter is likely blocked! See 8. Trouble Shooting
Release the vacuum
After complete ventilation of the unit, first take off the filter plate
a
and then
the manifold collar
b
.
Finally remove the receiver plate
l
containing the filtrates
To
obtain
the
best
possible
vacuum, the whole plate has to
be sealed independent off the
number of filled wells. Make sure
the foil is tightly put on the surface
of the filter plate. We recommend
to use a fresh foil for every
filtration.
After the pump starts, press the
filter
plate
a
a
a
a
shortly but tightly to
the manifold collar (
b
to ensure
tight fitting of the plate. The pump
stops automatically after ~ 3
minutes, the time required for
filtration of 200
µ
L plasma.
To release the vacuum, press the
ventilation valve button
f
f
f
f
. The
pressure will fall to 0 at the
pressure gauge
h
.
The vacuum pump inside the unit
will generate a vacuum of ~ - 15
inHg (i.e. ~ - 45 kPa), The
pressure will be indicated on the
pressure gauge
h
.
Note: Filter plates and plate sealer
are intended for single use only !
Note: Receiver plates are intended
for single use only !
Содержание Ceveron MFU 500
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