CONTROLLED COPY
Centre for Biological Engineering
Standard Operating Procedure
SOP025
Title: USE AND MAINTENANCE OF THE SYSTEC VX 95 AUTOCLAVES
Location:
Autoclave Room, H31, CBE
Version 004
Effective Date: 28
th
June 2020
Review 28
th
June 2022
Written by: A. Chandra
Reviewed by: P.Hourd
Approved by: R.I.Temple
Page 25 of 26
Annex 1: Example of ‘dummy waste bags’ used for Autoclave validation ( dry waste )
• 1 x 2m long 1.6 inner bore tube
• 2 x pipette tip boxes
• 5 x T-175 flasks with bags
• 10 x bijous
• 20 x 1000ml pipette tips
• 20 x 5000ml pipette tips
• 1 x pipette tip box
• 3 x pipette tip box packaging
• 10 x large gloves
• Cardboard glove box
• 3 x T-175 flasks with bags
• 5 x 25 ml centrifuge tubes
• 5 x 20ml centrifuge tubes
10 x bijous Bag 3
Annex 2 Example of ‘dummy waste bags’ which go into for buckets due to
having some liquid content
.
• 40 agar plates
• 3 x 25ml pipettes
• 3 x 50 ml pipettes
• 3 x 30ml bijous
• 3 x weighing boats
• 10 x Pasteur pipettes
• 1 x T-75 flasks
• Packaging of the flasks, pipettes
• 3 x 50 ml centrifuge tubes
• 2 x small bottle
• 1 pair of small gloves
•
Annex 3 . The load for validation of waste cycle No 6 (liquids) for the Autoclave by Systec included
• 1 x 5L bottle with water
• 2 x 2L bottles with water (one with probe)
• 1 x 1L bottles with water
