ProtoCOL 3
2-Sector Spiral Batch Set Up - Quick Guide
Start ProtoCOL 3 software
Log on to ProtoCOL 3
Capture image
Select the image tab and check the
live box
Create batch
Click New Batch
Position plate
Insert the plate holder, ensuring the
plate holder that gives the best
contrast between the colony and
background is used
Adjust the exposure by increasing/
decreasing the sliding scale
Capture image
Click
Click
Enter
Slide
Click
Plate configuration
Select Plate Configuration to set
plate size, sample volume and
threshold. Defaults are Circular
plate 90mm, Spiral volume
100µl and threshold of 20.
Change if necessary
Select
Select
Choose an application
Select Spiral Plate
1
2
3
4
5
6
Place plate onto plate holder
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