Red coloured shading indicates overexposure of white. Blue coloured shading indicates
underexposure. The optimum exposure time is one showing no red shading.
The InGenius LHR has a very wide dynamic range which means that higher levels of light can be
detected by the camera before it reaches saturation point.
To check for saturation on a grabbed image, click on the Histogram icon.
A graph will be displayed indicating which grey shades have been detected by the camera, from 0
- 4095 (or 0-65535 if EDR has been used). Providing that all areas of the image are within this
range and within the linear range of the camera, quantification may be performed with confidence.
Please note that for dyes that have a lower dynamic range compared to the camera e.g. silver or
coomassie then it will not be possible to utilise the full dynamic range of the camera.
16) Auto exposure
17) Printing
18) Saving an image
19) UV safety override
Auto exposure
- InGenius LHR has a unique auto-exposure system leaving the software to decide
the optimum camera settings to "grab" the best image. The image will have no saturated areas and
is fully quantifiable. This setting should only be used when capturing images of fluorescent
samples. It is especially useful when the gel is dark or if the bands are very faint. To activate this
function simply click on the AUTO EXPOSE button.
Depending upon the gel this can take several seconds until the camera has found the optimum
settings. (A progress indicator shows the automatic exposure control is operating).
Once it has completed the process the "grabbed" image is displayed in the image window and the
lighting is automatically switched off.
Printing
- When an image is "grabbed" the PRINT icon is available.
Click this to send a copy of the image to the thermal printer
for a hard copy output.
Saving the image
- You have the choice of either selecting FILE/SAVE AS or FILE/EXPORT. In
the first case the image can be saved in the Syngene secure SGD format for true GLP. With the
second option, the image can be saved in a range of formats including TIF, BMP, GIF, PCX, TGA,
WPG, and JPEG. Images can then be exported to other software packages.
For more detailed information concerning capture and enhancement including custom user
configurations, annotation, filter and colour overlays, image browser etc. - please refer to the full
manual supplied.
UV Safety override
-To use the transilluminator with the door open override the UV safety
switch by pulling down the safety interlock switch which is located above the door opening on the
right.
Note
- Please take adequate precautions to ensure that the UV light cannot reach your eyes or
skin or anyone else’s by wearing the appropriate protective equipment.
Note
- The interlock will be re-set when the door is closed.
* If both the
focus indicator
and
increased effective pixel
features are not present then install
GeneTools software as these features are licensed with the analysis software.
S Y N G E N E
A DIVISION OF THE SYNOPTICS GROUP
CONTACT SYNGENE:
EUROPE
IN USA:
BEACON HOUSE
5108 PEGASUS COURT, SUITE M
NUFFIELD ROAD
FREDERICK
CAMBRIDGE
MD 21704
CB4 1TF
Tel: +44 (0)1223 727123
Tel: 800 686 4407/301 662 2863
Fax: +44 (0)1223 727101
Fax: 301 631 3977
Email: [email protected]
Email: [email protected]
Email: [email protected]
Email: [email protected]
www.syngene.com
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IGLHRINST0408
