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Although there is no recommended setting for this friction, it should be noted that if there is
no friction at all, the joystick would always fall to the center position under gravity, which
may not be desirable. Similarly, if the friction is set too tightly, the joystick may become too
stiff to use or may be locked in place and could be damaged if the dial is tightened further.
With a little practice, a suitable tension setting can be established.
1.4.2.7
‘Work’ Position
The users’ ‘Work’ position can be designated as any position within the range of travel of the
micromanipulator. It is used primarily to ensure that the micropipette tip is not
inadvertently lost from the microscope field of view. It is recommended that the ‘Work’
position is refreshed at regular intervals during the course of an injection experiment. The
micropipette can be returned to the last position memorized by briefly touching the ‘Work’
key (See Operating Instructions).
1.4.2.8
‘Home’ Position
Activation of the ‘Home’ function moves the mechanical to its outermost and uppermost
position, with respect to the microscope. The ‘Home’ position of a right-hand
micromanipulator will be all the way up and to the right and the ‘Home’ position of a left-
hand micromanipulator will be all the way up and to the left. It is for this reason that a right-
hand micromanipulator should not be used on the left side of the microscope and vice-versa.
The ‘Home’ position is a factory preset and cannot be re-configured. See Operating
Instructions for details.
1.4.2.9
Speed
The speed of the micromanipulator can be adjusted to accommodate different microscope
magnifications and the differing needs of operators and applications. There are six speed
settings corresponding to six different sensitivities of micromanipulator movement. See
Operating Instructions on how to set the desired movement range. Speed settings 1 and 2 are
good for locating pipette into field of view. Speeds 3, 4, and 5 are ideal for micromanipulation
and microinjection.
1.4.2.10
‘Z-floor’
The ‘Z-floor’ is a function which allows the user to define a lower limit (or “floor”) of the Z-
axis travel of the micromanipulator. In practice, it is used to protect a micropipette from
being broken by preventing the user from crashing the micropipette into the bottom of the
microinjection chamber. The ‘Z-floor’ is described in more detail in the Operating
Instructions section.
1.4.2.11
Micropipette Holder Clamp
The micropipette holder clamp arrangement on the front of the mechanical is designed to
allow many different micropipette types to be used with the maximum stability possible. The
clamp can be adjusted for height, reach and angle of attack. See Operating Instructions for
details on setting the micropipette holder clamp for the optimum angle of attack of the
micropipette.
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