STEMCELL RoboSep-S, Руководство пользователя

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1.0 Introduction
RoboSep™-S is the second generation of RoboSep™, a fully automated cell separator based on column-free
EasySep™ technology. EasySep™ is an immunomagnetic cell separation technology that enables both
positive and negative selection of desired cells from virtually any species, sample source, or size. Using an
integrated color touchscreen, RoboSep™-S can be programmed to separate cells from up to four samples
simultaneously and can also sequentially isolate different cell types from the same sample. The user simply
loads the cell sample and reagents into the carousel and starts the experiment. RoboSep™-S automatically
executes the EasySep™ immunomagnetic labeling and magnetic separation steps using disposable pipette
tips, eliminating cross-contamination. After completion of the cell separation cycle, the cells of interest are
immediately available for any application.
1.1 EasySep™ Magnetic Cell Separation Technology
EasySep™ is a powerful immunomagnetic cell separation procedure that combines the specificity of
monoclonal antibodies with the simplicity of a column-free magnetic system. EasySep™ can be used as
either a negative or a positive selection approach to purify your cells of interest.
For
EasySep™ negative selection applications, unwanted cells are targeted for depletion using a cocktail of
monoclonal antibodies directed against specific cell surface antigens. The labeled unwanted cells are then
cross-linked to EasySep™ magnetic particles. The sample tube containing the cells is placed in a specially
designed EasySep™ magnet. The desired (unlabeled) cells are removed either by inversion of the magnet
(manual EasySep™) or by pipetting off the supernatant (RoboSep™-S).
With
EasySep ™ positive selection , highly purified cells are obtained by targeting the cells of interest with
monoclonal antibodies directed against one or more specific cell surface antigens. These targeted cells are
cross-linked to EasySep™ magnetic particles. The sample tube containing the cells is then placed directly in
a specially designed EasySep™ magnet. The cells that are not bound to the magnetic particles are removed
either by inversion of the magnet (manual EasySep™) or by pipetting off the supernatant (RoboSep™-S).
Unlike larger particles used with other cell separation systems, the EasySep™ magnetic particles used in
positive selection procedures do not interfere with subsequent flow cytometry and do not need to be removed.