4
STEINDORFF
®
4)
When observing the microstructure of a specimen, usually use a low power objective to look for the object,
then move the object towards the center of the field of view and exchange a high power objective for
observation. Care should be taken to avoid the objective being knocked against the specimen and damaged.
When focusing, lift the stage allow it close the objective lens as near as possible.
Lower the object surface while viewing. When an image is observed, use the fine adjustment knob for
adjustment until a sharp image is obtained.
5)
Examining the specimen in orthogonal polarized light. As mentioned in section (6) when a sharp image is
obtained, because the polarizer is still inserted in the optical path, actually it is in a single polarized light.
Then insert an analyzer. Pay attention to the graduated marks of the polarizer and analyzer. When both
marks are situated at “0” position, it means that they are in orthogonal. Now, the polarized orientation of
the analyzer is in north - south (or up and down).
6)
Examining the specimen in the conical polarized light. Usually, conical polarized light is used for high
power examination. Insert Bertrand lens into the light path when using orthogonal polarizing light. The
swinging upper lenses of the condenser are arranged so that they may be moved into the optical axis of the
microscope for examining the conical characteristic of the specimen.
7)
When an indistinct image of the filament of the bulb reveal in the field of view, add a ground glass
properly. When the light is yellowish, a filter can be added. (Place a filter above the collector in the base of
the microscope in transmitted light).
8)
Adjusting the light housing.
When the bulbs is replaced, the light housing should be realigned. Insert the light housing into the adapter
of the vertical illuminator. Switch on the power, moves the light housing back & forth to eliminate the
image of the filament in the field of view. After operating as mentioned in Section (6) then observe through
eyepiece tube with the eyepiece removed, until a bright spot at the back focal plane of the objective or the
image of the aperture diaphragm can be seen.
Adjust screws at the rear and side surface for centering the filament of the bulb.
USE OF BERTRAND LENS IN POLARIZING MICROSCOPE
The Bertrand Lens is generally used to observe interference figures in high power objective. Please check the
following points while using Bertrand Lens.
