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HPLC

Model 210D

9.

  Load the LC.CON control file from your PeakSimple program folder. This control file contains all the

necessary information to configure PeakSimple for HPLC.

10.

  Verify that the correct I/O port is

specified in the 

Edit/Overall

 screen. By

default, COM 1 is entered in the 

Edit/Overall

screen because many Windows

TM

computers have COM 1 designated as the
serial port. Other computers may use COM
2, COM 3, or COM 4. You may have to
examine the 

My Computer/System

 screen

to determine what serial port numbers
Windows has assigned to the hardware in
your particular computer. If you upgraded
to the 6 channel USB data system, enter
the USB device ID number in the box
instead.USB device ID numbers are unique
to each instrument, and the number for your
210D is printed on the HPLC under the cable port, and also on your PeakSimple disk. Once you have
successfully gotten the data system to sign on, click Save All so you don’t have to repeat this step the next time
you open PeakSimple.

8.

  

Double-click on the PeakSimple icon to launch the program.  Verify that

communication is established between the computer and the pump.  An error
message (shown at left) will appear if communication is not established.

7.  Installing PeakSimple software

You will find your PeakSimple

installation disk just inside the

front cover of your manual.
Install the PeakSimple

software by inserting the

PeakSimple CD into the CD-

ROM drive of your Windows

TM

computer.  Double-click on My
Computer, then open the CD-ROM
drive.  Double-click on the “setup.exe”
program icon and follow the
instructions.

Содержание 210D

Страница 1: ...nd a 100 L glass injection syringe SGE part 005300 HPLC Model 210D UV detector lamp switch Rheodyne 7725i injection valve Pressure gauge Prime purge valve Column compartment thumbscrews Storage compar...

Страница 2: ...s onto the PEEK tubing letting about 1mm of PEEK tubing protrude Slidethecolumnthroughthecolumnheatersoyoucanreachtheotherend andattachthesecondnut Roughlycenterthecolumninthecolumnheater Replace the...

Страница 3: ...lve The 1 4 Tygon tubing attached to the pump head s isthepistonflushline Pistonflushing helps keep the high pressure seal s clean minimizingwearandtearfromcrystallizedand abrasivebufferdepositsthatle...

Страница 4: ...ng Alength of tygon tubing is provided to connect the pump to the degas fitting on the left hand side of the HPLC The tubingisequippedwithanutforeasyconnectiontothedegasfitting With the 210D power ON...

Страница 5: ...box instead USBdeviceIDnumbersareunique toeachinstrument andthenumberforyour 210D is printed on the HPLC under the cable port and also on your PeakSimple disk Once you have successfullygottenthedatas...

Страница 6: ...y on channel 3 and the detector cell temperaturewilldisplayonchannel4 12 Setting a Gradient Program Click on the Gradient button for channel 1 If your system is isocratic the Inital and Final percenta...

Страница 7: ...pressure should begin building to operating range which is dependent upon the flow rate solvent viscosity and the back pressure of the column selected When the system stabilizes at the operating pres...

Страница 8: ...e documentation in your SRI manual under HPLCInjectorsformoreinformationaboutoperatingtheinjectionvalve HPLC Model 210D The detector exit tube that connects to the top of the solvent recycle valve may...

Страница 9: ...0mL minute Pump A 100 methanol Pump B 99 75 methanol with 0 25 acetone Events Time Event 0 00 Zero100 HPLC Model 210D ExpectedPerformance Step Gradient This chromatogram shows the 210D UV detector res...

Страница 10: ...chromatogram is a UV separation of 5ppm BTX with an isocratic 210D system The second chromatogram shows the same run after using PeakSimple s data smoothing function Short term up down noise is a func...

Страница 11: ...r smoothing ExpectedPerformance UV Detector Noise This pair of raw and smoothed chromatograms shows 20 minutes of a UV detector noise run on an isocratic 210Dsystem Inthelongertimesegment thesignaldri...

Страница 12: ...Turn ON the UV lamp switch Thesignalshouldjumpto3 6mV Thissignalis100 transmittance or0absorbanceunits Clickon the Zero 100 button to zero the signal with just the solvent flowing this is 100 transmi...

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