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HPLC

Model 210D

This pair of chromatograms shows a short-term, one minute UV detector noise run on an isocratic 210D
system.  The first chromatogram is the raw data, and the second shows it after smoothing.

Expected Performance

UV Detector Noise

This pair of raw and smoothed chromatograms shows 20 minutes of a UV detector noise run on an isocratic
210D system.  In the longer time segment, the signal drift is visible.

UV detector noise

averages

approximately

100µV from peak to

peak, or 10

-4

 a.u.

(absorbance units).

This UV detector

signal drift

averages

approximately

1.3mV in 20

minutes, or

0.0013 a.u.

Содержание 210D

Страница 1: ...nd a 100 L glass injection syringe SGE part 005300 HPLC Model 210D UV detector lamp switch Rheodyne 7725i injection valve Pressure gauge Prime purge valve Column compartment thumbscrews Storage compar...

Страница 2: ...s onto the PEEK tubing letting about 1mm of PEEK tubing protrude Slidethecolumnthroughthecolumnheatersoyoucanreachtheotherend andattachthesecondnut Roughlycenterthecolumninthecolumnheater Replace the...

Страница 3: ...lve The 1 4 Tygon tubing attached to the pump head s isthepistonflushline Pistonflushing helps keep the high pressure seal s clean minimizingwearandtearfromcrystallizedand abrasivebufferdepositsthatle...

Страница 4: ...ng Alength of tygon tubing is provided to connect the pump to the degas fitting on the left hand side of the HPLC The tubingisequippedwithanutforeasyconnectiontothedegasfitting With the 210D power ON...

Страница 5: ...box instead USBdeviceIDnumbersareunique toeachinstrument andthenumberforyour 210D is printed on the HPLC under the cable port and also on your PeakSimple disk Once you have successfullygottenthedatas...

Страница 6: ...y on channel 3 and the detector cell temperaturewilldisplayonchannel4 12 Setting a Gradient Program Click on the Gradient button for channel 1 If your system is isocratic the Inital and Final percenta...

Страница 7: ...pressure should begin building to operating range which is dependent upon the flow rate solvent viscosity and the back pressure of the column selected When the system stabilizes at the operating pres...

Страница 8: ...e documentation in your SRI manual under HPLCInjectorsformoreinformationaboutoperatingtheinjectionvalve HPLC Model 210D The detector exit tube that connects to the top of the solvent recycle valve may...

Страница 9: ...0mL minute Pump A 100 methanol Pump B 99 75 methanol with 0 25 acetone Events Time Event 0 00 Zero100 HPLC Model 210D ExpectedPerformance Step Gradient This chromatogram shows the 210D UV detector res...

Страница 10: ...chromatogram is a UV separation of 5ppm BTX with an isocratic 210D system The second chromatogram shows the same run after using PeakSimple s data smoothing function Short term up down noise is a func...

Страница 11: ...r smoothing ExpectedPerformance UV Detector Noise This pair of raw and smoothed chromatograms shows 20 minutes of a UV detector noise run on an isocratic 210Dsystem Inthelongertimesegment thesignaldri...

Страница 12: ...Turn ON the UV lamp switch Thesignalshouldjumpto3 6mV Thissignalis100 transmittance or0absorbanceunits Clickon the Zero 100 button to zero the signal with just the solvent flowing this is 100 transmi...

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