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1.  Overview

The Model 210D front panel houses the UV detector lamp switch, Rheodyne injection valve, prime/purge
valve, pressure gauge, and the storage
compartment.  In the storage compartment
are the LED display for the column
temperature, and the vacuum degas pressure

gauge (shown above).  An accessories kit is
included with your 210D.  In the kit are two
PEEK finger tight nuts (Alltech part # 32233)
for column connection, a spare 1/16”
stainless steel fitting (Valco part ZN1) and
ferrule (Valco part ZF1S6), a flange fitting
(Upchurch part 203X) and ferrule
(Upchurch part 240X) for the solvent recycle valve, a 20mL priming syringe (Sigma Aldrich part Z248037),
and a 100µL glass injection syringe (SGE part 005300).

HPLC

Model 210D

UV detector
lamp switch

Rheodyne 7725i injection valve

Pressure gauge

Prime/purge

valve

Column

compartment
thumbscrews

Storage
compartment

On the right hand side of the 210D is the column compartment, which gives you access to the column heater,
pump head(s), solvent recycle valve, and the detector housing.  Open the column compartment by loosening
the two captive thumbscrews and lifting off the cover.  Use the picture below to familiarize yourself with the
interior of the column compartment.

Rheodyne 7725i
injection valve
with a 20µL
sample loop

Pressure
gauge

Prime/purge
valve

Column heater

Solvent recycle valve

UV and

Conductivity

detectors

Vacuum de-gas coil

Pulse damper

Vertical

slide

Pump inlet lines

Pump heads

Pump outlet line

Vacuum

de-gas

outlet

Содержание 210D

Страница 1: ...nd a 100 L glass injection syringe SGE part 005300 HPLC Model 210D UV detector lamp switch Rheodyne 7725i injection valve Pressure gauge Prime purge valve Column compartment thumbscrews Storage compar...

Страница 2: ...s onto the PEEK tubing letting about 1mm of PEEK tubing protrude Slidethecolumnthroughthecolumnheatersoyoucanreachtheotherend andattachthesecondnut Roughlycenterthecolumninthecolumnheater Replace the...

Страница 3: ...lve The 1 4 Tygon tubing attached to the pump head s isthepistonflushline Pistonflushing helps keep the high pressure seal s clean minimizingwearandtearfromcrystallizedand abrasivebufferdepositsthatle...

Страница 4: ...ng Alength of tygon tubing is provided to connect the pump to the degas fitting on the left hand side of the HPLC The tubingisequippedwithanutforeasyconnectiontothedegasfitting With the 210D power ON...

Страница 5: ...box instead USBdeviceIDnumbersareunique toeachinstrument andthenumberforyour 210D is printed on the HPLC under the cable port and also on your PeakSimple disk Once you have successfullygottenthedatas...

Страница 6: ...y on channel 3 and the detector cell temperaturewilldisplayonchannel4 12 Setting a Gradient Program Click on the Gradient button for channel 1 If your system is isocratic the Inital and Final percenta...

Страница 7: ...pressure should begin building to operating range which is dependent upon the flow rate solvent viscosity and the back pressure of the column selected When the system stabilizes at the operating pres...

Страница 8: ...e documentation in your SRI manual under HPLCInjectorsformoreinformationaboutoperatingtheinjectionvalve HPLC Model 210D The detector exit tube that connects to the top of the solvent recycle valve may...

Страница 9: ...0mL minute Pump A 100 methanol Pump B 99 75 methanol with 0 25 acetone Events Time Event 0 00 Zero100 HPLC Model 210D ExpectedPerformance Step Gradient This chromatogram shows the 210D UV detector res...

Страница 10: ...chromatogram is a UV separation of 5ppm BTX with an isocratic 210D system The second chromatogram shows the same run after using PeakSimple s data smoothing function Short term up down noise is a func...

Страница 11: ...r smoothing ExpectedPerformance UV Detector Noise This pair of raw and smoothed chromatograms shows 20 minutes of a UV detector noise run on an isocratic 210Dsystem Inthelongertimesegment thesignaldri...

Страница 12: ...Turn ON the UV lamp switch Thesignalshouldjumpto3 6mV Thissignalis100 transmittance or0absorbanceunits Clickon the Zero 100 button to zero the signal with just the solvent flowing this is 100 transmi...

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