Rev. 2.2
April 2021
SPI-DRY
TM
Critical Point
Dryer
Operation Manual
SPI Supplies Part #13200JE-AB
Страница 1: ...Rev 2 2 April 2021 SPI DRYTM Critical Point Dryer Operation Manual SPI Supplies Part 13200JE AB...
Страница 2: ...1 800 2424 SPI Toll free from USA Canada 1 610 436 5400 FAX 1 610 436 5755 E mail spi3spi 2spi com For further information regarding any of the other products designed and manufactured by SPI Supplies...
Страница 3: ...r use of your unit according to the operation manual should result in trouble free operation Any improper use of the SPI Supplies unit through modifications or unreasonable operating procedures will v...
Страница 4: ...n Introduces this manual Section 4 General Description Identifies each of the equipment items and provides an overview of their functions and how they work Section 5 Installation Instructions on how t...
Страница 5: ...unctionality Signs 10 2 4 5 Serious Damage to Instruments 10 2 4 6 Hazard to Operator 10 2 5 Risk Analysis 11 2 5 1 Personal Operational Risks 11 2 5 2 Hazardous Materials 11 2 6 Good Working Practice...
Страница 6: ...Preparation 23 6 3 1 Washing 24 6 3 2 Fixation 25 6 3 3 Dehydration 25 6 3 4 Substitution 26 6 4 Complete Trial Operation Run 26 Section 7 Maintenance 28 7 1 Chamber Components 29 7 1 1 Viewing Window...
Страница 7: ...in this Manual 10 Figure 2 5 Typical Warnings as shown in this Manual 10 Figure 6 1 Phase Diagram 21 Figure 6 2 Process Flow Chart 24 Figure 7 1 Cross section of SPI DRY Critical Point Dryer 28 Figur...
Страница 8: ...nstrument or modification of the instrument 2 2 Conformity This instrument is supplied in a form that complies with the protection requirements of the EC Electromagnetic Compatibility Directive 89 336...
Страница 9: ...ock Caution refer to accompanying documents Easily touched higher temperature parts Warning risk of electric shock Figure 2 1 Hazard Warning Symbols 2 4 3 Hazard Warning Labels used in Equipment Manua...
Страница 10: ...anual This typical caution sign is used where serious damage will be caused by incorrect operation of instrumentation They will follow the same form as functionality warnings but with a triangular war...
Страница 11: ...ts before use in the vacuum system It should be noted that isopropanol is a flammable liquid and as such should not be used on hot surfaces In addition it is recommended that protective gloves be worn...
Страница 12: ...bend from the knees when lifting heavy objects Wear protective clothing when using liquid nitrogen Affix pressurised gas cylinders firmly to walls or racks Use the correct regulating valves on gas cyl...
Страница 13: ...ier costs This covers defects which arise as a result of a failure in design or manufacturing It is a condition of warranty that equipment must be used in accordance with the manufacturers instruction...
Страница 14: ...t Dryer Temperature and Pressure gauges High pressure CO2 transfer hose Tubing for water connections Tissue Baskets and Holder Tool Kit Spare Parts Kit Operation Manual 4 1 1 Accessories The following...
Страница 15: ...to the water jacket to lower the temperature of the chamber to below 20C Samples that have previously been chemically dehydrated are loaded into the chamber which is then filled with liquid CO2 A ser...
Страница 16: ...e ends of the water jacket are sealed with two annular end plates and gaskets Two water connectors are screwed into the vessel wall one at each end to allow for connection of the water supply Both end...
Страница 17: ...e preparation is carried out with the samples in the baskets to the point where final substitution is made pure ethanol acetone amyl acetate etc At this point the boat is filled with the substitution...
Страница 18: ...ragraph 3 1 5 1 Unpacking Checklist The Equipment package will normally be despatched from the factory in one box Inside the box the following will be found refer and check each item off against the s...
Страница 19: ...sure transfer hose to the INLET valve of the pressure vessel The fiber washer is necessary to ensure a good seal Tighten with a wrench Connect the other end of the high pressure transfer hose to the C...
Страница 20: ...orphology and ultra structure Since liquid CO2 is not sufficiently miscible with water it is necessary to use an intermediate fluid which is miscible with both water and liquid CO2 In practice interme...
Страница 21: ...the TEMPERATURE and PRESSURE gauges As the temperature of the chamber nears 30C the pressure should rise to approximately 1100 psi Once at this point the surface of the liquid will start to dissolve...
Страница 22: ...llege London The sequence of operations outlined is very comprehensive In many cases it may be possible to omit some of the stages With difficult specimens however care taken in fixation and dehydrati...
Страница 23: ...ing medium must be physiological i e must not cause any changes in shape or form of the tissue Glutaraldehyde Fixation Load into CPD Chamber At 100 C or less Fill Chamber With Liquid CO2 Osmium Post F...
Страница 24: ...h routes if any give the best results It may well be that they all give similar quality The most convenient method is route a as it takes the shortest time and uses the cheapest transition fluid Route...
Страница 25: ...om the chamber fill the chamber with liquid and close all of the gas control valves Allow 1 hour for the liquid glass to impregnate the samples Please note this time is only a guide for tissue samples...
Страница 26: ...door and remove the liquid transfer boat with the now dried samples t Mount the samples on the desired mount and prepare them for examination in the SEM or other instrument NOTE If the system is to b...
Страница 27: ...mance it is recommended that the following procedures be carried out on a regular basis a Regularly clean the inside and outside surfaces of the pressure vessel including the O ring and associated sea...
Страница 28: ...se b Unscrew and remove the DRAIN valve and adapter plug c Remove the insert and O ring for inspection and cleaning d Re assemble in reverse order using a new O Ring and bonded seal 7 1 4 Gas Control...
Страница 29: ...ing is carried out rapidly The use of a thermal transfer grease between the thermometer stem and the pressure vessel will reduce this lag b Pressure Gauge a Using a well fitting open end wrench on the...
Страница 30: ...g We hope that you experience the minimum of problems throughout the lifespan of the instrument but inevitably problems may occur Any known problems associated with this type of instrument have been l...
Страница 31: ...as they occur To maintain the equipment to the best operating conditions a maintenance schedule is suggested as part of a fault prevention programme the following items are suggested to be included i...
Страница 32: ...umbo Safety Valve Bonded Seal EPDM fits both Regular and Jumbo Window O Ring Nitrile fits both Regular and Jumbo Window O Ring EPDM fits both Regular and Jumbo Control Valve Seating O Ring Nitrile Con...
