8
eDNA SAMPLER
www.smith-root.com
VOLUME OFFSET
This value needs to be changed when-
ever the tubing length or filter arrange-
ment is altered (for example, switching
from 1 filter housing to 3 on the transect
pole). To calculate the volume offset
for the current setup, use the following
procedure with an assistant:
1. Fill a bucket or reservoir with clean
water.
2. In manual mode, start the pump and
fill the tubing and system until a solid
stream of water is visible emitting
from the “out” tube, with no bubbles.
3. Stop the pump and press the reset
button on the interface.
4. Invert the filter housings by rotating
pole, while retaining as much water
in the extension tubes as possible.
5. Press the start button on the inter-
face and allow the pump to run until
all water has been cleared from the
tubing and pump. Then press the
stop button.
6. The “Liters” value displayed (e.g.,
300ml) can now be used for the
“Volume Offset” for the new filter
and tubing arrangement.
7. To confirm the setup, switch to
auto mode and follow the sam-
pling procedure (inverting filters at
2-beeps). The target volume should
be achieved with the new Volume
Offset value.
