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eDNA SAMPLER
www.smith-root.com
I
MPORTANT:
Crack the seal (do not open) and allow the pump to run for approx-
imately 20 seconds to air dry the filter membrane. Excess moisture will prevent
thorough desiccation and eDNA preservation.
4. Remove the extension tube and discard in an appropriate field waste container.
5. Place the self-preserving filter housing back into the original packaging. Min-
imize excess moisture on the outside of the filter housing. A light shake can
remove water droplets.
6. Reseal the foil pouch with the zip-type sealing strip. The filter housing material
will immediately begin preserving the captured eDNA by desiccation.
7. Label the sample bag and place sample in a field storage container at ambient
temperature.
8. Multiple samples can be aggregated and stored at room temperature (70°F,
21°C) until bulk laboratory processing. Current data support 6 months of pres-
ervation before DNA extraction.
9. Once in the laboratory for DNA extraction, remove the filter housing with the
preserved 47mm eDNA membrane from the storage bag.
10. Open the filter housing using the pull-tab and reveal the eDNA filter mem-
brane.
11. The eDNA filter membrane can then be removed from the housing using for-
ceps for DNA extraction. The filter backer will remain stuck to the housing. All
elements other than the eDNA filter membrane can then be discarded.
STATEMENT OF BIODEGRADATION
Smith-Root self-preserving filter housings contain a material that is recognized as
one of very few synthetic polymers which are water soluble and “inherently” bio-
degradable in water.
The polymer manufacturer’s internal experimental results indicate it is “inherently”
biodegradable in water when the degree of hydrolysis is higher than 70 mol%.
•
Test method; ISO 14851
•
Sludge concentration; 100 mg / L
•
Sample concentration; 100 mg / L
In aqueous solution, adapted microorganisms are capable to mineralize the poly-
mer into carbon dioxide and water.
For more information, visit:
https://besjournals.onlinelibrary.wiley.com/doi/full/10.1111/2041-210X.13212
