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Laboratory Centrifuge Sigma 6-16S

6 Using the centrifuge

Version 07/2012, Rev. 1.22 of 08/05/2020

• sb

39 / 91

Translation of the original operating manual, part no. 0702002

→

6.3.2.5

Standard menu

The "Standard" menu is symbolised by the icon " " on the menu bar and it
is displayed a few seconds after the centrifuge has been switched on. In
this menu, the parameters of a centrifugation run can be displayed and
modified.

Fig. 14: Standard menu; here shown with all of the possible symbols

Speed

In the upper section of the field, the set speed of the centrifuge is displayed.
The actual speed is displayed below this value. The values are stated in
revolutions per minute (min

-1

= rpm) and depend on the RCF values (see

chapter 2.2.2.1 - "Speed, radius, and relative centrifugal force"). The
maximum speed values depend on the rotor that is used.

Relative centrifugal force (RCF)

The relative centrifugal force is the acceleration that the sample is
subjected to during the centrifugation run. The set value of this parameter is
displayed in the upper section of this field, with the actual value shown
below. The values are stated in g (gravitational acceleration) and they
depend on the speed values (see chapter 2.2.2.1 - "Speed, radius, and
relative centrifugal force"). The maximum RCF values depend on the rotor
that is used.

Runtime

The set runtime is displayed in the upper section of this field, with the
remaining runtime shown below. The runtime is defined as the period from
the start of the centrifuge to the beginning of the deceleration phase. The
maximum value is 99 h 59 min 59 sec.

In the "Setup" menu

, it can be specified that the runtime is not to be

started until the set speed is reached (see chapter 6.3.2.8 - "Setup menu").
In this case, the symbol "



!

" appears in the runtime field.

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Содержание 6-16S

Страница 1: ...slation of the original operating manual sb 07 2012 Rev 1 22 of 08 05 2020 Sigma 6 16S part no 0702002 Sigma 6 16S from serial no 147414 Laboratory Centrifuge Operating Manual Please retain for later...

Страница 2: ...Laboratory Centrifuge Sigma 6 16S 2 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002...

Страница 3: ...0 sb 3 91 Translation of the original operating manual part no 0702002 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 5522...

Страница 4: ...Laboratory Centrifuge Sigma 6 16S 4 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002...

Страница 5: ...force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety inst...

Страница 6: ...of an angle rotor with a hermetic lid 30 6 2 2 3 Installation of accessories 32 6 2 2 4 Adapters 34 6 2 2 5 Vessels 34 6 2 2 6 Blood bag systems 35 6 2 2 7 Centrifugation with tubes for mineral oil 3...

Страница 7: ...1 4 Load bearing bolts 64 8 1 5 Glass breakage 65 8 2 Sterilisation and disinfection of the rotor chamber and accessories 65 8 2 1 Autoclaving 66 8 3 Service 67 8 4 Return of defective centrifuges or...

Страница 8: ...Laboratory Centrifuge Sigma 6 16S Table of contents 8 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002...

Страница 9: ...use Centrifuges are power driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyond...

Страница 10: ...uplicated distributed or communicated in any other way Non compliance may be prosecuted under criminal law 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declaration of...

Страница 11: ...perating manual part no 0702002 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1 User interface 3 Mains swit...

Страница 12: ...0702002 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9 CE mark in compliance with the directive 2006 42...

Страница 13: ...n of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Das...

Страница 14: ...la If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted whil...

Страница 15: ...sk of bruising Name plate see chapter 2 1 2 Name plate Protective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not dispose as part of domestic waste g lock System i...

Страница 16: ...d to the life and health of persons Non observance of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health...

Страница 17: ...rifuges The operator must perform a risk assessment concerning potential accidents in connection with the centrifuge and take design related countermeasures if necessary The centrifuge has to be maint...

Страница 18: ...dards 3 6 2 Mechanical safety In order to ensure the safe operation of the centrifuge observe the following WARNING Do not open the lid when the rotor is in motion Do not reach into the rotor chamber...

Страница 19: ...safety If pathogenic toxic or radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines precauti...

Страница 20: ...st the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installa...

Страница 21: ...ed for reasons of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the engraving max cycles 10 000 has...

Страница 22: ...ions to be drawn concerning the production process and the subsequent quality inspection Some rotors also have an additional serial number providing further detailed information The batch number and s...

Страница 23: ...the microprocessor 3 7 3 System check An internal system check monitors the data transfer and sensor signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as e...

Страница 24: ...hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules However danger to life and limb of the operator or of third partie...

Страница 25: ...ear Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If you would like to store it for more than one year or if you intend to ship it overseas please...

Страница 26: ...aging for any possible future transport of the centrifuge 4 5 Transport safety device The transport safety device consists of two plastic screws which are located at the bottom panel CAUTION The trans...

Страница 27: ...olid even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the...

Страница 28: ...operating manual part no 0702002 5 2 2 Customer provided fuses Typically the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer NOTE To ensure safe disconne...

Страница 29: ...se press with both hands slightly on the lid until the lock is audibly locked WARNING Do not place your fingers between the lid and the housing when closing the lid Risk of crushing 6 2 2 Installation...

Страница 30: ...tor with a hermetic lid Open the centrifuge lid by pressing the lid key Unscrew the rotor tie down screw from the motor shaft counter clockwise Screw the hermetic lid onto the rotor and tighten it clo...

Страница 31: ...Translation of the original operating manual part no 0702002 Removing a rotor Loosen the rotor tie down screw anti clockwise and remove the rotor together with the lid Loosen the lid by turning it ant...

Страница 32: ...mpartments must be equipped with buckets Always load rotationally symmetrical compartments of the rotors with the same accessories and fill to avoid imbalance Centrifugation with vessels of various si...

Страница 33: ...and their suspensions are loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 9 Permissible and impermissible loading of an angle rotor and a swing out rot...

Страница 34: ...s of a carrier are not used the buckets must be loaded evenly Loading the edges of a bucket only is not permissible 6 2 2 5 Vessels Load the vessels outside of the centrifuge Liquids in the buckets or...

Страница 35: ...ag systems must have an equal weight The blood bags have to be put into the opposite buckets mirror inverted If in one bucket the main blood bag is on the left side then the main blood bag inserted in...

Страница 36: ...7 21 2 min 11 or 2 min 6 Platelets with buffy coat 22 1 094 384 7 21 2 min 11 or 3 5min 7 Platelets with buffy coat 1 only with special equipment heater 2 Curves no 20 21 and 24 are freely programmabl...

Страница 37: ...on knob The display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start key 2 Display 3 Function knob 4 Stop...

Страница 38: ...fore a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Quick stop will be...

Страница 39: ...peed values depend on the rotor that is used Relative centrifugal force RCF The relative centrifugal force is the acceleration that the sample is subjected to during the centrifugation run The set val...

Страница 40: ...centrifuge accelerates with acceleration curve 9 maximum acceleration until the maximum speed of the rotor is reached The runtime is counted and in the speed field the message Short run flashes When t...

Страница 41: ...tically identify the correct rotor and select the rotor bucket combination with the lowest speed The system will display a corresponding message so that the combination can be adapted manually If the...

Страница 42: ...the programs please see chapter 6 3 3 Program mode Fig 16 Program list Progress indicator The progress indicator provides a quick overview of the remaining runtime of the running centrifugation run Fo...

Страница 43: ...sponds to their storage locations on the program list Empty storage locations will not be displayed If more than 11 programs have been stored the user can scroll through the list Fig 18 Process librar...

Страница 44: ...If the density of the liquid to be centrifuged is higher than 1 2 g cm3 the value must be adapted manually in order to prevent the glass vessel from breaking This will reduce the maximum possible fin...

Страница 45: ...ctive the drive will be disconnected if the actual speed is below the set speed As a result the rotor decelerates and stops in a brakeless manner HINWEIS A spin out in particular with heavy rotors and...

Страница 46: ...git code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock level will be indicated If changes are made after a function has been blocked the sys...

Страница 47: ...speed If this function is active the runtime will not be measured until the set speed is reached In the Standard menu the symbol will be displayed in the runtime field NOTE In the program mode this fu...

Страница 48: ...he set speed in steps of 1 rpm instead of 100 rpm the RCF value in steps of 1 x g instead of 10 x g and the set time in steps of 1 min or 1 sec instead of 10 min or 10 sec NOTE Regardless of the fine...

Страница 49: ...ress the knob again in order to enter the next character Pressing the arrow button will delete the last character The maximum number of characters is 19 When the name is complete select the option Acc...

Страница 50: ...If the curve number is already used the stored curve will be displayed The input field Int is used to specify the interval number of the process Up to ten intervals can be entered for a curve Enter th...

Страница 51: ...ssible speed depending on the rotor and from the slope limitation Example 1 Start speed 0 min 1 rpm final speed 100 min 1 rpm runtime 1 hour not possible since the necessary slope 0 03 min 1 rpm which...

Страница 52: ...nge Fig 25 Dialog box for changing the contrast 6 3 3 Program mode A program contains all of the data that are required for a centrifuge run Certain sedimentation results can be repeated under identic...

Страница 53: ...a letter and press it to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm...

Страница 54: ...desired program from the list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The progr...

Страница 55: ...ed while the program rotation function is active this program will be used as the start program for the rotation After the completion of the program the next program on the program list will be loaded...

Страница 56: ...ions for data input and output Connection for a serial interface depending on the centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A...

Страница 57: ...f on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly Open and close the lid If the error occurs again contact service Ce...

Страница 58: ...with a screwdriver Fig 31 Position of the openings for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be guided through a funnel shaped tube...

Страница 59: ...Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance monitoring system Al...

Страница 60: ...alfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen de Ou...

Страница 61: ...s Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8 1 1 Centrif...

Страница 62: ...after each use Adapters should be removed cleaned and dried CAUTION Do not clean the accessories in a dishwasher Cleaning in a dishwasher removes the anodised coating the result is cracking in areas...

Страница 63: ...re and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weaken the material without any visible signs Check the material regularly at least onc...

Страница 64: ...Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bolts and bucket groove in order to remove the old grease Apply a small...

Страница 65: ...order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a mode...

Страница 66: ...ycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carriers 115 118...

Страница 67: ...nents were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of...

Страница 68: ...e a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the outside of t...

Страница 69: ...002 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packagin...

Страница 70: ...ermissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of dec...

Страница 71: ...ravitational field x g Max kin energy Nm 13 500 3 200 20 376 105 901 Other parameters Time range Storage locations 10 sec 99 h 59 min short run continuous run 60 Physical data Height mm Height with op...

Страница 72: ...g manual part no 0702002 10 2 Technical documentation For environmental reasons the comprehensive technical documentation of the centrifuge e g circuit diagrams and the safety data sheets of the manuf...

Страница 73: ...ome supplied together with a data sheet that includes important information and notes on safety This data sheet must be added to the operating manual 11 1 1 Rotor radii The information in the accessor...

Страница 74: ...fuge Sigma 6 16S 11 Appendix 74 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002 11 2 Speed gravitational field diagram Fig 36 Speed gravitatio...

Страница 75: ...y 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power The runtime until the set speed is reached depends solely on the moment of inertia of t...

Страница 76: ...Centrifuge Sigma 6 16S 11 Appendix 76 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002 Fig 39 Diagram of linear curves Fig 40 Diagram of quadra...

Страница 77: ...rs 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12097 5 years 20x 1...

Страница 78: ...4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1...

Страница 79: ...C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N 10...

Страница 80: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nitr...

Страница 81: ...1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1...

Страница 82: ...Laboratory Centrifuge Sigma 6 16S 11 Appendix 82 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002...

Страница 83: ...Laboratory Centrifuge Sigma 6 16S 11 Appendix Version 07 2012 Rev 1 22 of 08 05 2020 sb 83 91 Translation of the original operating manual part no 0702002 11 6 EC declaration of conformity...

Страница 84: ...Laboratory Centrifuge Sigma 6 16S 11 Appendix 84 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002...

Страница 85: ...Laboratory Centrifuge Sigma 6 16S 11 Appendix Version 07 2012 Rev 1 22 of 08 05 2020 sb 85 91 Translation of the original operating manual part no 0702002 11 7 Declaration of conformity China RoHS 2...

Страница 86: ...Laboratory Centrifuge Sigma 6 16S 11 Appendix 86 91 Version 07 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0702002...

Страница 87: ...6 42 EC 15 Centrifugation monitoring 44 Centrifugation of blood bag systems 35 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation wit...

Страница 88: ...ease 58 Error correction 57 Error message 57 Executing a program 54 Explanation of the symbols and notes 16 Explosive substances 19 External signal 49 F Fire preventions 19 Form for the return of defe...

Страница 89: ...38 Motor error 59 Motor shaft 29 30 61 Multiple carrier 34 N Name plate 11 12 No indication on the display 57 No power in the mains supply 57 Noise level 71 Nominal voltage 12 Notes on safety and haza...

Страница 90: ...rical 18 Safety mechanical 18 Safety conscious work 17 Saving a program 53 Scope of supply 10 Screws of the transport safety device are not removed 57 Selection display and modification of data38 Seri...

Страница 91: ...Thermal stress 61 Time fine 48 Time range 71 Toxic substances 19 61 Transport safety device 26 Tubes 34 Type 12 71 Type of connection 27 Type of the centrifuge 60 67 U Unblocking a function 46 Ungreas...

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