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Operating Manual SIGMA 4-15C, page 48 of 95

03/06

4.2.7.4 Density

Density

If the density of a sample exceeds 1.2 g/cm

, the maximum final speed will

reduce (refer to chapter 9.4.2

“

Density

”

). A value between 1.2 and 9.9 g/cm

possible.

4.2.7.5 Start Delay

With this function a start delay can be set.

4.2.7.6 Automatic Lid Opening after End of Run

If this parameter has been set the lid will automatically open after standstill of the
rotor.

Содержание 4-15C

Страница 1: ...Centrifuge 4 15C Operating Manual...

Страница 2: ......

Страница 3: ...instrument has a program memory which can store up to 50 data sets and is capable of keeping the last run program in its memory for an unlimited amount of time allowing the program to be restarted at...

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Страница 5: ...98 37 CEE La machine d sign e ci dessous est produit et examin conforme aux directives 73 23 CEE 89 336 CEE et 98 37 CEE Bezeichnung der Maschine Laborzentrifuge Machine Laboratory Centrifuge D signa...

Страница 6: ......

Страница 7: ...ty devices 2 6 1 Lid lock cover closing device 2 6 2 Imbalance monitoring system 2 6 3 Rotor monitoring 2 6 4 Standstill monitoring 2 6 5 System check 2 6 6 Ground wire check Installation and Start up...

Страница 8: ...lay and alteration of program parameters 5 1 1 Selection and alteration of the parameters and activation of the start delay and the automatic lid opening after end of run 5 1 2 Selection and alteratio...

Страница 9: ...cessories 8 6 1 Autoclaving 8 7 Checks by operator Appendix 9 1 Slope of the specified curves linear curves 9 2 Quadratic curves 9 3 Entry limitations 9 4 Mathematical relations 9 4 1 Relative Centrif...

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Страница 11: ...tion curves Deceleration curves Radius Rotor part no 9 h 59 min continuous operation 50 No 1 50 10 linear No 0 9 10 quadratic No 10 19 10 freely programmable No 20 29 9 linear No 1 9 1 brakeless No 0...

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Страница 13: ...0 cm radius min 10 0 cm max plate height 56 mm incl plate holder 17979 4 500 3 758 3 622 2 264 13231 Bucket aluminium for 1 pointed bottom bottle 150 or 200 ml e g 15175 approx 61 5 x 140 mm incl adap...

Страница 14: ...Polysulfone sealing cap clear for 13350 17350 Round carrier for 24 reaction vials 0 5 0 75 ml 7 9 10 x 28 31 mm e g 15005 polypropylene 17351 Round carrier for 12 Monovettes max 15 5 18 x 50 75 mm po...

Страница 15: ...ated up to 20 ml with skirt incl cap max 25 31 x 65 95 mm polypropylene see www bibby sterilin co uk no 03008 17370 Round carrier for 5 tubes 25 30 ml max 25 4 29 x 85 115 mm e g 15025 15026 15029 150...

Страница 16: ...propylene 17402 Round carrier for 1 tube with pointed bottom 150 250 ml max 60 x 120 140 mm polypropylene 17347 Round carrier for 1 bottle with screw cap 250 ml max 61 5 x 90 125 mm e g 13255 15247 15...

Страница 17: ...for 18002 for 20 reaction vials 1 5 2 2 ml max 11 mm e g 15008 15040 can be used together with 18002 polypropylene 18005 Rectangular carrier for 20 RIA tubes 5 ml max 12 2 x 60 75 mm flat and round b...

Страница 18: ...polypropylene see www bibby sterilin co uk no 03008 18030 Rectangular carrier for 5 tubes with screw cap 27 30 ml max 25 4 27 5 x 80 110 mm e g 15029 15030 15032 polypropylene 18050 Rectangular carri...

Страница 19: ...15022 15023 15024 decantable polyallomer 18125 Rectangular carrier for 1 bottle with screw cap 125 ml max 51 x 90 115 mm e g 15125 polypropylene 18200 Rectangular carrier for 1 bottle with screw cap...

Страница 20: ...8 12165 Angle rotor 6 x 78 85 ml for sealed tubes e g 13085 plus 17185 15074 15075 15076 15080 incl hermetic aluminium lid max radius 9 8 cm min radius 2 7 cm angle 25 12 500 17 119 12166 Angle rotor...

Страница 21: ...s 4 3 cm angle 25 12 500 18 168 13060 Adapter for 1 culture tube 15 ml 15115 suitable for 12169 polypropylene 12170 Angle rotor for 12 culture tubes 15 ml e g 15115 incl hermetic aluminium lid max rad...

Страница 22: ...65 polypropylene 13084 Adapter for 2 tubes 10 12 ml max 16 17 5 x 75 x 90 mm e g 15000 15010 15039 suitable for 12165 polypropylene 15005 Reaction vials 0 5 ml 7 9 10 x 28 31 mm 1 pack contains 100 pc...

Страница 23: ...Falcon tube with screw cap 15 ml pointed bottom polypropylene suitable for 12170 13060 13081 13236 17359 17360 18016 15029 Teflon tube with screw cap 28 ml 25 3 x 96 mm suitable for 12172 13083 17370...

Страница 24: ...5 38 40 x 100 107 mm suitable for 12165 17385 18085 17185 Stainless steel sealing cap for 13085 15074 Polycarbonate tube with special screw cap made of aluminium with seal for high speeds 72 ml 38 x 1...

Страница 25: ...cap 17256 61 4 x 125 mm suitable for 12256 17347 17256 Stainless steel sealing cap for 13255 15247 Teflon bottle with screw cap 250 ml 61 4 x 122 mm suitable for 12256 17347 15248 Polycarbonate bottl...

Страница 26: ...ss tube 50 ml 34 x 100 ml suitable for 17375 18050 15056 ditto graduated 4 50 ml in steps of 1 ml 15100 Glass tube 100 ml 44 x 100 mm suitable for 17390 18100 15106 ditto graduated 1 100 ml in steps o...

Страница 27: ...clip for caps 17108 17112 17801 Lid for rotor 12256 17919 Table for centrifuge made of varnished sheet steel with 2 doors and space for accessories movable on lock type castors dimensions w 490 d 635...

Страница 28: ...Operating Manual SIGMA 4 15C page 28 of 95 03 06...

Страница 29: ...unnion pins Part No 70 284 20 ml slushing oil Part No 70 104 Documentation 1 Short operating instructions 1 Operating Manual 1 Rotor and Accessories Operation and Use 1 EU Statement of Conformity 1 Eq...

Страница 30: ...the max speed of the centri fuge must be reduced 4 Operation of the centrifuge in hazardous locations is not allowed 5 During operation the centrifuge must not be moved Leaning against or resting on...

Страница 31: ...ion consulter les documents joints Ein Netzverbindung On Power Marche mise sous tension Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschlu Protective earth ground Liaison la terr...

Страница 32: ...ntrifuge is built into a sheet steel housing The armoured chamber the sheet steel lid the motorized lid lock device and the hinge system are providing optimum safety At the back the lid is secured by...

Страница 33: ...es due to the instrument s mechanical design there are the following active precautions for your safety 2 6 1 Lid Lock Cover Closing Device The centrifuge can only be started when the power switch is...

Страница 34: ...rammed rotor no STOP is carried out and an error message is displayed Restarting the centrifuge is only possible after reset and when the correct rotor number has been selected 2 6 4 Standstill Monito...

Страница 35: ...y Device The SIGMA 4 15C has a transport safety device which must be removed before start up The transport safety device blocks the drive system The transport safety device screws are accessible from...

Страница 36: ...time for drying before the centrifuge can be started again 3 2 2 Connection Fuse The operating voltage on the name plate must correspond to the local supply voltage SIGMA laboratory centrifuges are u...

Страница 37: ...he Configuration Cycles This ensures a proper connection between rotor and shaft please refer to chapter 8 2 Care and cleaning of accessories as well 5 Fill all positions of swing out rotors with buck...

Страница 38: ...the rotor fixing screw must always be checked using a wrench 3 3 1 Fastening of Angle Rotors with Hermetically Sealed Lid Please refer to drawing on next page 1 Screw rotor lid 2 onto rotor and tighte...

Страница 39: ...Operating Manual SIGMA 4 15C page 39 of 95 03 06...

Страница 40: ...display indicates 2 The program display indicates i e the centrifuge will accelerate to 2000 rpm the centrifuge operation is terminated after 2 minutes no program number has been allocated so far 3 4...

Страница 41: ...Operating Manual SIGMA 4 15C page 41 of 95 03 06 4 1 Operating Panel Operating panel The centrifuge can be operated via the operating panel Keys can be pressed when their LED is on...

Страница 42: ...tion curve The centrifuge can be started when the lid is closed the Start key is illuminated 4 1 2 Stop key This key can be used to early terminate a run The centrifuge decelerates with the preset cur...

Страница 43: ...ction of the area the set speed of the centrifuge is displayed Underneath is the actual speed The maximum speed values are rotor dependent 4 2 3 Relative Centrifugal Force RCF RCF The relative centrif...

Страница 44: ...and seconds The actual value has the same units as the set value and is displayed in hours minutes or in minutes seconds if the set value is below 10 minutes h m m s If the highest possible time of 9...

Страница 45: ...load stored programs without calling the selection list The program used last is automatically loaded after restarting Program Selection List Program selection list The area marked with arrows for the...

Страница 46: ...isplayed Rotor Selection List Rotor selection list The area marked with arrows for the rotor selection list offers all available rotors which can be selected via the knob 4 2 7 Parameters PARA Paramet...

Страница 47: ...r 9 1 Slope of the specified curves linear curves and 9 2 Quadratic curves 4 2 7 2 Deceleration Deceleration The deceleration number selects a deceleration curve that decelerates the centrifuge down t...

Страница 48: ...the maximum final speed will reduce refer to chapter 9 4 2 Density A value between 1 2 and 9 9 g cm3 is possible 4 2 7 5 Start Delay With this function a start delay can be set 4 2 7 6 Automatic Lid...

Страница 49: ...uration Configuration Selection of configuration CONFIG opens a menu where Code Language Screen Fine Buzzer Sensor Info Reset and the creation of freely programmable curves can be chosen Furthermore t...

Страница 50: ...the alteration mode SET and the selected area are inverted Select the new value of the selected area by turning the knob Confirm the entry by pressing the knob You then leave the alteration mode SET a...

Страница 51: ...by turning the knob selection mode and confirm by pressing the knob The parameters to be changed e g acceleration deceleration radius density and the start delay and automatic lid opening after end of...

Страница 52: ...START DELAY by turning the knob Activate the START DELAY by pressing the knob Select time delay here 10 seconds by turning and confirm by pressing the knob Change the set time by turning the knob and...

Страница 53: ...b After pressing the knob you get a view over all available rotor types Rotor selection list Select the number of the actual rotor bucket combination by turning the knob and confirm the entry by press...

Страница 54: ...tching into the continuous run mode time mode Acceleration curve Deceleration curve Start delay Alter the Set values as usual by activating the Alteration mode refer to chapter 5 1 Selection Display a...

Страница 55: ...speed or the RCF display FINE In this function it is possible to preselect the set speed in steps of 1 or 10 min 1 The set time can be preselected in steps of 1 min or 1 sec Exeption Curve input in 1...

Страница 56: ...s the possibility to delete all programs parameters and configurations to get the original settings again CURVES Creation of user defined accelerations and decelerations The procedure of alteration of...

Страница 57: ...screen marked with a cross is activated If you want to select the screen SPEED ZOOM you proceed as follows Select SPEED ZOOM by turning the knob Activate the Speed Zoom screen by pressing the knob Sel...

Страница 58: ...Operating Manual SIGMA 4 15C page 58 of 95 03 06 By pressing the knob you return to the main display The areas SPEED and TIME are displayed magnified The area RCF disappears...

Страница 59: ...n be prohibited CUT Speed in one interval is automatically limited to the set speed for the run ADMIT Chosen speed in one interval is allowed CANCEL A stop occurs Starting is not possible without to a...

Страница 60: ...2 Creation of Curves for Variable Accelerations and Decelerations Select the configuration mode by turning the knob After pressing the knob the configuration menu appears Select CURVES by turning the...

Страница 61: ...95 03 06 Select 0 00 00 by turning the knob You are now creating interval no 1 Activate 0 00 00 by pressing the knob Set the time by turning the knob Save the time value in interval no 1 by pressing t...

Страница 62: ...the knob Change the speed value by turning the knob The created curve with the max curve speed appears After pressing and turning the knob the time value of interval no 2 appears Activate 0 00 00 of...

Страница 63: ...6 Save the time value no 2 by pressing the knob Select SPEED by turning the knob Activate the speed value 0 by pressing the knob Change the speed value by turning the knob After pressing and turning t...

Страница 64: ...ng Manual SIGMA 4 15C page 64 of 95 03 06 Activate 0 00 00 of the third interval by pressing the knob Set the time by turning the knob The extended curve appears Save the time value no 3 by pressing t...

Страница 65: ...Change the speed value by turning the knob The curve appears and can be programmed as acceleration or deceleration curve under no 20 Select EXIT by pressing and turning the knob and leave the curve mo...

Страница 66: ...ars Now you can select the contrast by turning the knob By pressing the knob again you confirm the entry the new contrast remains 5 1 6 Imbalance Monitoring An imbalance dialogue window indicates an e...

Страница 67: ...y the shortrun function is activated The instrument accelerates to the maximum speed with the maximum acceleration curve and after release decelerates with the maximum deceleration curve until standst...

Страница 68: ...y entry errors Programs can be loaded operated altered and deleted any time All programs can be protected against unauthorized use by a personal Code 6 1 1 Load a Program There are two possibilities o...

Страница 69: ...knob and activate this area by pressing the knob You are able to see all programs in the memory indicates the actual program Program selection list Select the program you want to load by turning the k...

Страница 70: ...by turning the knob and activate it by pressing the knob to get a view over all programs in the memory and the free positions in the list Program selection list Select the desired program position in...

Страница 71: ...lect the program selection list by turning the knob and activate this area by pressing the knob to see the list of programs Program selection list Select the program you want to delete by turning the...

Страница 72: ...sition of the bucket only is not allowed see illustration Even angle rotors should be loaded symmetrically to same weight Centrifugation with different tubes Working with different tube sizes is possi...

Страница 73: ...ealed rotors and buckets only 17 Do not spin explosive or highly imflammable materials 18 Record all program data refer to forms of appendix chapter 9 8 19 When centrifuging substances with a density...

Страница 74: ...mogeneous density of 1 2 g cm3 if centrifuged at max speed If liquids of higher density are used the speed must be reduced accordingly refer to chapter 9 4 Mathematical relations 7 Operation with roto...

Страница 75: ...otor is spinning 17 Such materials are prohibited which chemically interact vigorously 18 Do not spin explosive or inflammable materials 19 Substances which could damage the material of the centrifuge...

Страница 76: ...contamination agents which had not been recommended by us the user has to contact us to make sure that such procedure would not damage the centrifuge Remove product particles from the rotor chamber us...

Страница 77: ...has to contact us to make sure that such procedure would not damage the centrifuge The rotor tie down screw must be greased using the supplied grease SIGMA part no 70284 Cleaning of accessories shoul...

Страница 78: ...e minutes at a moderate speed The glass and metal particles will now collect at the greased part and can easily be removed with a cloth together with the grease If necessary repeat this procedure 8 5...

Страница 79: ...0 20 Polypropylene tubes 115 118 30 40 30 Teflon tubes 134 138 3 5 100 Aluminium rotors 134 138 3 5 Polypropylene rotor 12034 115 118 30 40 20 Polypropylene rotor 12124 115 118 30 40 20 Polycarbonate...

Страница 80: ...or suspension 2 Concentricity of the motor shaft 3 Fastening of the trunnions in the rotor 4 Rotors and accessories have a limited life For safety reasons a regular check is recommended after 50 000 c...

Страница 81: ...the rotor to 1000 rpm With linear and quadratic slopes curves are numbered in the direction of increasing acceleration from right to left The deceleration curves are inverted images of the accelerati...

Страница 82: ...ntrifuge accelerates with max torque The acceleration time only depends on the mo ment of inertia of the rotor Linear curve no Slope 0 240 sec 1000 rpm 1 180 sec 1000 rpm 2 120 sec 1000 rpm 3 60 sec 1...

Страница 83: ...um torque The acceleration time only depends on the moment of inertia of the rotor Quadratic curve no Time up to 1000 rpm Slope from 1000 rpm linear 10 240 120 sec 1000 rpm 11 180 90 sec 1000 rpm 12 1...

Страница 84: ...splayed 9 4 Mathematical Relations 9 4 1 Relative Centrifugal Force RCF The parameters speed RCF and the diameter of the rotor are interrelated via the following formula RCF 11 18 x 10 6 x r x n2 If t...

Страница 85: ...r Change contrast 9 5 1 Centrifuge cannot be Started a Start key LED not illuminated Close lid Motorized lid locks must close b Start key LED illuminated Power off on If error occurs again call servic...

Страница 86: ...of a power supply failure it is possible to manually open the lid Remove stopper at the upper right side front panel using e g a screw driver Unlock the motorized lid lock using the supplied square w...

Страница 87: ...ation power off on 85 Excess temperature centri fuge chamber 50 C let cool down provide for better ventilation power off on 87 Actual temperature 45 C resp 20 C provide for better ventilation 90 96 Se...

Страница 88: ......

Страница 89: ...me copies before removing this page Attention This form must be glued on outside of the box Return declaration YES NO Decontamination declaration inside Unit component contaminated Unit component unus...

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Страница 91: ...___________________________________________________________ with gloves only _________________________________________________________________ General characteristics of the substances Corrosive Explo...

Страница 92: ......

Страница 93: ...Operating Manual SIGMA 4 15C page 93 of 95 03 06 9 9 Program No Used for Alteration by Date Speed Gravitational field Rotor Bucket Made on by...

Страница 94: ......

Страница 95: ...Operating Manual SIGMA 4 15C page 95 of 95 03 06...

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