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Operating Manual SIGMA 3-16K, page 52 of 69
03/06
5.1
Practical Notes for Centrifugation
1. Locate centrifuge horizontally on a level surface.
2. Ensure safe location.
3. Keep at least 30 cm free space around the centrifuge.
4. Provide for sufficient ventilation.
5. Tighten rotor firmly onto motor shaft.
6. Avoid imbalance.
7. Load opposite buckets with same accessories.
8. Centrifugation with low capacity:
The example shows a complete swing-
out rotor 16 x 15 ml which is loaded with
8 vessels only. The vessels must be
loaded symmetrically so that the buckets
and their suspensions are loaded evenly.
Loading an eg. only an outer position of
the bucket is not allowed.
8.1Centrifugation with different tubes:
Working with different tube sizes is
possible. Opposite places must be
loaded with the same vessels.The
example shows a swing-out rotor with
buckets and 2 x carriers 100 ml and 2 x
carriers 4 x 15 ml with tubes.
8.2 Another example is the fixed angle rotor
24 x 2,2 ml:
The vessels should be placed symmetri-
cally so that the rotor is loaded evenly.
Loading e.g. only one position is not
allowed.
9. Load all positions of swing-out rotors.
10. Load vessels outside the centrifuge.
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