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Refrigerated Centrifuge Sigma 2-16KL

Version 08/2012, Rev. 1.24 of 28/04/2022

• sb

3 / 82

Translation of the original operating manual, part no. 0700702

+49 (0) 5522 / 5007-0

Fax:

+49 (0) 5522 / 5007-12

Web: www.sigma-zentrifugen.de
E-mail: [email protected]

Содержание 2-16KL

Страница 1: ...ation of the original operating manual sb 08 2012 Rev 1 24 of 28 04 2022 Sigma 2 16KL part no 0700702 Sigma 2 16KL from serial no 146109 Refrigerated Centrifuge Operating Manual Please retain for late...

Страница 2: ...Refrigerated Centrifuge Sigma 2 16KL 2 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Страница 3: ...022 sb 3 82 Translation of the original operating manual part no 0700702 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 55...

Страница 4: ...Refrigerated Centrifuge Sigma 2 16KL 4 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Страница 5: ...gal force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Requirements concerning the personnel 18 3...

Страница 6: ...gle rotor with a hermetic lid 31 6 2 2 3 Installation of the microhaematocrit rotor 32 6 2 2 4 Installation of accessories 33 6 2 2 5 Adapters 35 6 2 2 6 Vessels 35 6 3 Control system Spincontrol L 36...

Страница 7: ...carriers 55 8 1 4 Microhaematocrit rotor 55 8 1 5 Load bearing bolts 56 8 1 6 Glass breakage 57 8 2 Sterilisation and disinfection of the rotor chamber and accessories 57 8 2 1 Autoclaving 58 8 3 Serv...

Страница 8: ...Refrigerated Centrifuge Sigma 2 16KL Table of contents 8 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Страница 9: ...odule 010 Allgem eine Inform ationen 010 0020 Bestimm ungsgem e Verw endung Sigma 26 m od_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separate liquids...

Страница 10: ...N ormen und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations These operating instructions have been created in accordance with the relevant European standards and re...

Страница 11: ...ufbau und Wirkungsw eise 020 0010 Aufbau der Zentrifuge 26 m od_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 2 16KL_2 16KH L 020 Au...

Страница 12: ...2 16KL 29 m od_1405321347233_68 docx 196609 3 1 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9 CE mark in...

Страница 13: ...bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Pos 23 010 U niversalmodule Leerz...

Страница 14: ...force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an...

Страница 15: ...fuge I On Power Arrow indicating the direction of rotation 0 Off Power Rotor loading information see chapter 2 1 2 Name plate Name plate see chapter 6 2 2 4 Installation of accessories CE mark in comp...

Страница 16: ...e of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observan...

Страница 17: ...l health and safety regulations as well as with the accident prevention regulations must be checked at regular intervals e g every month Under the international rules for health and safety at work the...

Страница 18: ...ised persons are present in the danger zone or work area This manual uses the following personnel qualifications for various areas of activity Qualified electrician Due to their special training knowl...

Страница 19: ...the location of use of the centrifuge Ensure that it is accessible at all times The operating manual must be handed over to any subsequent owner or operator of the centrifuge Any changes additions or...

Страница 20: ...ich the housing needs to be removed Inspect the electrical equipment of the unit regularly Defects such as loose or burnt cables must be eliminated immediately Following the completion of any type of...

Страница 21: ...319025698_68 docx 192073 3 1 3 6 3 Fire prevention DANGER Do not spin explosive or inflammable substances Do not use the centrifuge within hazardous locations Pos 49 010 U niversalmodule Leerzeile 0 m...

Страница 22: ...ssels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a rotor Observe the instructions on the installation of acces...

Страница 23: ...n etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor...

Страница 24: ...number see chapter 7 2 Table of error codes Pos 65 010 U niversalmodule Leerzeile 8pt 12 mod_1337063062114_0 docx 65687 1 Pos 66 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030...

Страница 25: ...tandar dmodule 030 Sicher heit 030 0100 R estrisiken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance wi...

Страница 26: ...4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If y...

Страница 27: ...1 Pos 88 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 2 16KL_2 16KH L 040 Lagerung und Tr ansport 040 0050 Transportsicherung 2 16KL_KH L berschrift 29 m od_1405321382496_68 docx 196679 2 1 4 5 Transp...

Страница 28: ...be stable and have a solid even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for dryi...

Страница 29: ...ule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 98 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 050 Aufstellung und Anschl uss 050 0020 0020 Sicherungen bauseits au er 3 30KS 8KS 8KBS 7 m od...

Страница 30: ...he lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid The centrifuge cannot be started if the lid is opened To...

Страница 31: ...r ol L S 060 0020 0020 0021 Einsetzen eines Wi nkelrotors mit H ermetikdeckel 3 Nm 29 mod_1405321531516_68 docx 197015 4 1 6 2 2 2 Installation of an angle rotor with a hermetic lid Open the centrifug...

Страница 32: ...63 4 1 6 2 2 3 Installation of the microhaematocrit rotor Open the centrifuge lid by pressing the lid key Replace the rotor tie down screw with the rotor tie down screw of the microhaematocrit rotor p...

Страница 33: ...oint in the capillary tubes You can now read the percentage value Remove the capillary tubes Some of the tubes can also be evaluated with the reader outside of the rotor see the instructions for use o...

Страница 34: ...enly It is not permissible to load angle rotors on only one axis correct incorrect Fig 7 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations NOTE Pay at...

Страница 35: ...060 0020 0020 0050 Gef e 2 16KL bis 4 5L 27 m od_1405319211622_68 docx 192577 4 1 6 2 2 6 Vessels Load the vessels outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosio...

Страница 36: ...5319250704_68 docx 192619 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several differen...

Страница 37: ...centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered fast will be displayed in th...

Страница 38: ...speed and RCF can be changed during the centrifugation Fig 11 Setting the speed value or the RCF value Pos 134 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 135 100 Sigma 100 BA...

Страница 39: ...bol disappears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed on the symbol Fig 13 The function...

Страница 40: ...entrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol L Zent6 060 0030 0020 0070 Tem per atur nur KL 27 m od_1405319266022_68 docx 192773 4 1 6 3 2 7 Temperature The set value is...

Страница 41: ...s the set temperature can be modified within the range below the actual temperature The Rapid Temp program will be stopped under the following conditions The set value is reached The program will stop...

Страница 42: ...al different types of possible buckets the various combinations will be displayed one after the other Confirm the input The selected rotor or rotor bucket combination will be adopted Fig 18 Preselecti...

Страница 43: ...erzeile 0 mod_1202116244500_0 docx 114 1 Pos 143 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol L Zent6 060 0030 0020 0100 Autom atische D eckel ffnung 2...

Страница 44: ...red setting The bar remains visible as long as the cursor is placed over the symbol Fig 21 The sound signal Buzzer is activated Pos 146 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1...

Страница 45: ...der quit the cycle display Fig 23 Cycle display for rotor 11650 and bucket 13421 Reaching the maximum number of cycles When the maximum number of cycles is reached for a rotor or bucket a correspondin...

Страница 46: ...activated Proceed in the same manner in order to deactivate the permanent input lock Fig 25 Padlock symbol indicating an activated input lock Pos 152 010 Universalmodul e Leerzeile 0 mod_120211624450...

Страница 47: ...are indicated by a flashing display Any storage locations that are already occupied will be overwritten during the saving process Save the program in the desired location The program is now saved NOT...

Страница 48: ...dm odul e 060 Betrieb_Spi ncontr ol L S 060 0040 Ausschalten 27 mod_1405319212716_68 docx 192591 2 1 6 4 Switching the centrifuge off Open the centrifuge when it is not in use so moisture can evaporat...

Страница 49: ...orrectly Fuses have tripped Reactivate temperature fuse see chapter 5 2 1 Type of connection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illum...

Страница 50: ...d Switch off the mains power switch and disconnect the power cord from the socket Remove the plug see figure item 1 from the opening at the side panel e g with a screwdriver Fig 28 Position of the ope...

Страница 51: ...s Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only fo...

Страница 52: ...ies malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen...

Страница 53: ...e g by heat generators Pos 174 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 175 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 080 War tung und Instandhaltung 080 0010 W ar...

Страница 54: ...0 War tung und Instandhaltung 080 0010 0020 Zubeh r au er 8K 28 m od_1405320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must be consider...

Страница 55: ...ds Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weak...

Страница 56: ...ased load bearing bolts ensure a uniform swing out of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to st...

Страница 57: ...e upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part...

Страница 58: ...s Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polya...

Страница 59: ...is high level of stress high quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber...

Страница 60: ...obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised p...

Страница 61: ...Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The...

Страница 62: ...ld waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defectiv...

Страница 63: ...Type 2 16KL Connection requirements Electr connection Protection class IP code see name plate I 20 Power consumption kW Input fuse AT 0 72 6 0 at 220 240 V 50 60 Hz temperature fuses Performance data...

Страница 64: ...allowable relative humidity of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 211 010 Universalmodul e Leerzeile...

Страница 65: ...downloaded from www sigma zentrifugen de Pos 217 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 218 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 219 100 Sig...

Страница 66: ...16KL 11 Appendix 66 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702 Fig 32 Microhaematocrit rotor Pos 220 010 Universalmodul e Seitenwechsel 0...

Страница 67: ...manual part no 0700702 Pos 221 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 m od_1405320705642_68 docx 195153 2 1 11 2 Speed gravitation...

Страница 68: ...eption is curve 0 It decelerates brakeless spin out In general the runtime until the set speed is reached depends on the moment of inertia of the rotor Linear curves The slope of the fixed acceleratio...

Страница 69: ...16KS 4 16KHS 6 16S 6 16HS 6 16KS 6 16KHS 9366 15 000 4 5KL 4 16S 4 16KS 4 16KHS 6 16S 6 16HS 6 16KS 6 16KHS 11805 10 years 8KS 8KBS 11806 10 years 8KS 8KBS 12082 7 years 1 14 1 14K 12083 7 years 1 14...

Страница 70: ...0 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702 Pos 227 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0050 Best ndigkei...

Страница 71: ...4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1...

Страница 72: ...ne C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N...

Страница 73: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nit...

Страница 74: ...1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4...

Страница 75: ...28 04 2022 sb 75 82 Translation of the original operating manual part no 0700702 Pos 229 100 Sigma 110 BA Zentrifugen Sigma Proj ekte 2 16KL_2 16KH L 110 Anhang 110 0060 Konformit tserkl rung 2 16KL_K...

Страница 76: ...Refrigerated Centrifuge Sigma 2 16KL 11 Appendix 76 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Страница 77: ...efrigerated Centrifuge Sigma 2 16KL 11 Appendix Version 08 2012 Rev 1 24 of 28 04 2022 sb 77 82 Translation of the original operating manual part no 0700702 11 7 Declaration of conformity China RoHS 2...

Страница 78: ...Refrigerated Centrifuge Sigma 2 16KL 11 Appendix 78 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702 Ende der Liste f r Textm arke Inhalt...

Страница 79: ...trifuge is inclined 49 Centrifuge was moved during run 49 Centrifuge cleaning and care 53 Centrifuges definition 9 CE Zeichen gem Richtlinie 2006 42 EG 15 Changes in colour 58 Changes in structure 58...

Страница 80: ...bearing bolts 53 56 H Hazard warnings 9 30 31 35 Heavy duty grease for load bearing bolts 56 Highly corrosive substances 21 I Imbalance 33 35 Imbalance error 51 Imbalance monitoring system 24 Importan...

Страница 81: ...evention of accidents 9 Problem description 60 Process error 51 ProgLock program lock 44 Program Rapid Temp 41 Program lock ProgLock 44 Protection class 28 63 Q Qualified electrician 18 Quick stop 37...

Страница 82: ...d accessories 57 Stopping of the centrifuge due to an imbalance 56 Storage and transport 26 Storage conditions 26 Storage locations 63 Stress corrosion see corrosion 55 Structural changes 21 Supply vo...

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