8
Analysis Procedure
WARNING
Extraction and analysis requires technical expertise
regarding chemical analysis.
Inexperienced operators should always perform
extraction and analysis work under the supervision
of an experienced operator or outsource the process
to an analysis and measurement service provider
qualified to do so. In addition, precautionary
measures, such as wearing protective gear or
preventing fires, are required for handling the
hazardous or highly volatile substances described
below. Therefore, obtain and reference an SDS or
other relevant safety information in advance.
z
Preparing Standard Solutions
WARNING
This product is designed for use with hazardous
and highly volatile substances. Implement
appropriate precautionary measures.
Prepare standard solutions by diluting commercial DNPH derivatives of
aldehydes and ketones with acetonitrile.
Formaldehyde (4
μ
g HCHO/mL)
Place 1 mL of the formaldehyde DNPH standard solution (40
μ
g
HCHO/mL from Wako Pure Chemical Industries) in a volumetric flask.
Add and dissolve acetonitrile to make a total volume of 10 mL.
z
Preparing Calibration Curves
WARNING
This product is designed for use with hazardous
and highly volatile substances. Implement
appropriate precautionary measures.
Prepare a series of about five dilution levels from each standard solution.
Inject the series of standard solution dilutions into the HPLC system and
measure the peak area (or peak height) of target substances.
Repeat this process to determine a mean value.
Calculate the slope and intercept of the regression line from the
concentrations of the solutions corresponding to the chromatogram peak
height or peak area values using the least squares method or some
Example
