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ver.211209E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 RSpak

 KC-811 6E 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 RSpak

 KC-811 6E is a high performance 

chromatography column which is specifically designed for the separations and analysis of organic acids. The 
column is packed with a strong acid cation exchange gel prepared from a rigid resin of styrene divinylbenzene 
copolymer and it works under ion exclusion chromatography mode. It is capable of separating and analyzing 
multiple organic acids simultaneously.   

 
2. Column Components   
 
 

                 

 

 

 
 
 
 
 
 
 

 
 

 

3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle size 

(

μ

m) 

Theoretical Plate Number 

(Per Column) 

I.D. Length 

F6378033 

RSpak KC-811 6E

 

6.0 250 

 

 13,000 

F6700030 

RSpak KC-G 6B

 

6.0 

 

50 10  (Guard 

Column) 

 

Base Material 

: Spherical porous particles of styrene divinylbenzene copolymer modified with sulfo group

 

Column Housing  : SUS-316 
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : 0.1 % Phosphoric Acid aq. Solution 
 

 

 

Содержание RSpak KC-811 6E

Страница 1: ...ackage column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex RSpak KC 811 6E is a high performance chromatography column which is specifically designed for the separations and analysis of organic acids The column is packed with a strong acid cation exchange gel prepared from a rigid resin of styrene divin...

Страница 2: ... or has been contaminated Always use freshly prepared solvents Solvents stored for a long time may have changed their compositions and may influence elution patterns and or damage the column Note Use of on line degasser is recommended 6 Sample Preparation 1 If possible use the eluent for analysis to dissolve or dilute samples If this is difficult use a solvent which has a composition that is as cl...

Страница 3: ...peaks 3 Set the initial flow rate at 0 2 0 3 mL min and start the system If using the column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired 4 At the end of analysis reduce the flow rate to 0 2 0 3 mL min and turn the column oven off Make sure that the column oven temperature...

Страница 4: ...moved by reversing the flow direction i e introducing the eluent from the column outlet with flow rate at less than half of the recommended flow rate 2 Follow below cleaning steps for adsorbing components For an efficient cleaning reverse the flow direction Method 1 Adsorption of ionic compounds cations Set the column temperature at 50 C and the flow rate at 0 5 mL min Introduce 25 mL of 25 mM aqu...

Страница 5: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Страница 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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