- 5 -
When a difference develops in the refractive index between the sample and the
Reference in the flow cell, the image on the photo sensor moves horizontally as
illustrated in Fig. 4.3 below ( moves from (a) to (b) ). The electric signals emitted
respectively from the photo sensor change in proportion to the extent of movement
of the image.
Thus, signal corresponding to the difference in refractive index can be obtained from
a difference between the two signal outputs.
(a)
No difference in the
refractive index
(b)
Some difference in the
refractive index
Fig. 4.3 Movement of the image on the photo sensor
4
4
4
4-
-
-
-2
2
2
2 Flow
Flow
Flow
Flow L
L
L
Line
ine
ine
ine
As shown in Fig 4.4 below, the flow line is so designed as to enable replacement of
a reference liquid by only pushing a button.
Fig. 4.4 Flow Line
1
3
2
1
2
3
316 stainless steel tubing
Teflon tubing
Connection by female nut
Connection by flange
1 :
Photo Sensor A
2 :
Photo Sensor B
3 :
Image
1 :
Heat exchanger
2 :
Flow cell
3 :
Sample cell
4 :
Reference cell
5 :
Heat exchanger
6 :
Joint block
7 :
3-way solenoid valve
8：Relief Valve
From column
To waste liquid receptacle
（Panel）
4
2
3
OUT
IN
COM
NO
NC
1
5
6
7
NO : Normally Open
NC : Normally Close
8
.
