Shodex Asahipak C4P series, Руководство пользователя

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Asahipak ODP-50 (C8P, C4P series)
Manual
5
4. Column installation
4.1 Direction of flow
Install and use the column with mobile phase flowing in the direction indicated on the column
tag.
If the column shows very high resistance to flow, it may be partially plugged up. This is probably
the end of the service life of the column. Some users have been successful in extending the life
somewhat by back flushing the column. The outlet of the column is connected to the injector so
the liquid flows into the normal outlet fitting. The other end, normally the column inlet is placed
into a beaker. The pump is then turned on at the minimum flow rate listed in Table 1. The
column is back flushed for about one hour to over night, or even longer, if the operating
pressure is declining. The column then should be retested according to the procedure on the
page "INSPECTION DATA" provided with each column. If the column is efficient, then one can
restore the column to operation in the instrument in the normal manner.
See also Section 5.1 on filtering and guard columns.
4.2 Connection tubes
For columns with diameters smaller than 10 mm id, use connection tubes from the injector and
to the detector that have the shortest practical length and internal diameter no larger than 0.3
mm, (0.010 inch) internal diameter. The outer diameter must be 1/16 inch, (0.0625 inch).
For preparative columns, (columns with internal diameters of 10 mm and larger), it is possible to
use connection tubes with internal diameter up to 0.5 mm, (0.020 inch). The length should be
kept as short as practical.
4.3 Pump
The use of pumps with strong pulsations in the output flow or pressure may shorten column life,
and should be avoided. Pulsation can often be reduced by using a pulse damper on the pump
outlet.
4.4 Preliminary purge
Before connecting the column to the system, purge the system and lines with liquid to remove
any air or particles.