260 91 000
Issue 1
26
th
May 2010
ECN 613
G:\DOCUMENT CONTROL\Manuals\Chroma\26091000 Operator Manual M260 Issue 1 #20864 on.doc
CHROMA M260
Colorimeter
Operator Manual
Copyright © 2010, Sherwood Scientific Ltd., All rights reserved.
Страница 1: ...ssue 1 26th May 2010 ECN 613 G DOCUMENT CONTROL Manuals Chroma 26091000 Operator Manual M260 Issue 1 20864 on doc CHROMA M260 Colorimeter Operator Manual Copyright 2010 Sherwood Scientific Ltd All rig...
Страница 2: ...Two Installation 7 2 1 Location 7 2 2 Services Required 7 2 3 Unpacking 7 2 4 Assembly 7 2 5 Connecting Peripheral Devices 7 2 5 1 Printer 7 2 5 2 Thermostatted Cuvette Holder 267 86 001 8 2 6 Instru...
Страница 3: ...ment Times 18 6 6 Automated Measurements 18 6 7 Sample Numbering 18 Chapter Seven Method Setup 19 7 1 Factor Method Setup Procedure 19 7 2 Concentration Method Setup Procedure 20 7 3 End Point Method...
Страница 4: ...ts to be used in conjunction with the instruments and the underlying principles of colorimetry Please note that the drop in Gelatin filters although sealed are subject to deterioration when exposed to...
Страница 5: ...r in UK or Overseas the first contact should be that Distributor for all issues of supply and operation Please note here the details of the Company where you purchased this unit Company Tel Number Con...
Страница 6: ...owered from a universal self adjusting power supply CARE should be taken to use the correct adapter for the user s local supply 1 4 Reagents The CHROMA range of Colorimeters is designed to be used wit...
Страница 7: ...ries consumables and spares 2 3 Unpacking The Model 260 Colorimeter is packed in a custom designed box which will protect the instrument for both air freight and sea freight transport The box contains...
Страница 8: ...20 2 6 Instrument Set up The CHROMA Model 260 is based on the very reliable and stable Sherwood design but now incorporates full programmability However it is still possible to operate the Simple prot...
Страница 9: ...a constant related to the amount of light absorbed per analyte molecule i e specific absorption This property has been given many names in the literature such as molar absorptivity and extinction coef...
Страница 10: ...4 2 Measurement Range When used with test kits the manufacturer of the kit will specify the individual measurement ranges depending on the particular assay 4 2 1 Operating Range 0 to 2 999 Abs 100 0...
Страница 11: ...e size The buttons were designed to be universally understood 5 1 3 Buttons The Select buttons These allow various choices to be displayed in the Menu sequence The Enter button Allows the user to sele...
Страница 12: ...e the end of the incubation measurement This allows the user to transfer the cuvette back to the colorimeter for the measurement Provision is made to enter the temperature of a particular test in the...
Страница 13: ...This only requires instrument with appropriate filter selected to be zeroed against a blank solution See 6 3 prior to measurement of samples Straightforward Factor and Concentration experiments may a...
Страница 14: ...orbance Transmission Recall New Time Format Date Format Set Clock Language Uart AutoPrint Contrast Run Edit Delete Concentration Factor Time Based Print Endpoint Kinetic NB Uart is for RS232 parameter...
Страница 15: ...Once the correct temperature has been reached and the Colorimeter has ended its warm up phase and shows Ready then the correct filter should be inserted When the appropriate filter has been put in the...
Страница 16: ...concentration of an analyte either by 1 Using a factor supplied by the test kit manufacturer 2 Comparison with a calibrator or standard of known concentration In the Model 260 Colorimeter s Simple Me...
Страница 17: ...Setup and Running of these method types is given in Chapter 7 and 8 respectively of the Manual 6 5 Time Based Measurements Some measurements particularly of clinical samples require time to develop a...
Страница 18: ...e Manual 6 5 3 Incubation and Measurement Times Many kinetic measurements procedures require an initial incubation time before a second reagent is added This time is sometimes referred to as the delay...
Страница 19: ...press Select Factor from Mode Menu using then press Select wavelength from list using then press Set Factor value Use and for each number column Note sample temperature if required using then This fac...
Страница 20: ...ard concentration value as outlined in the test kit Use then press for each number column Set concentration units using then Note sample temperature if required using then This facility only allows a...
Страница 21: ...n Use Calibrator screen using then press Set Factor value using then for each number column Set concentration units using then Set Incubation time using then for each number column Note sample tempera...
Страница 22: ...librator Screen using then press Set Calibrator value Use then for each number column Select required units using then for each unit s column Set Incubation time Use then for each number column Note s...
Страница 23: ...value Use then for each number column Select required units using then for each unit s column Set Incubation time Use then for each number column Set measuring period Use then for each number column N...
Страница 24: ...value using then for each number column Select required units using then for each unit s column Set Incubation time Use then for each number column Set measuring period Use then for each number colum...
Страница 25: ...from Recall Menu using then Select required Method using then Method details will move up and down in display Check details are correct and press Insert the filter as prompted then The cuvette holder...
Страница 26: ...re correct and press Insert the filter as prompted then The cuvette holder must be empty when you press Insert the blank in the cuvette holder and then press 0 The instrument will be set to zero Press...
Страница 27: ...filter as prompted then The cuvette holder must be empty when you press Put your sample and sample blanks in your pre heated bath or heater block and press to start the colorimeter timer Just before t...
Страница 28: ...nd down in display Check details are correct and press Insert the filter as prompted then The cuvette holder must be empty when you press Put the calibrator blank in a pre heated water bath or heater...
Страница 29: ...the sample number using then for each position When prompted put the sample blank and press the 0 The absorbance will be set to 0 000 Press enter Put the sample in the cuvette holder and press The sam...
Страница 30: ...he cuvette holder must be empty when you press Enter sample number using the then for each number Put the Sample in the preheated water bath or heater block and press to start the colorimeter timer Ju...
Страница 31: ...N 613 31 Kinetic Method with Factor Run Procedure continued The new optical density of the sample will be displayed Press The change in Sample OD per minute will be displayed Press The concentration o...
Страница 32: ...as prompted then The cuvette holder must be empty when you press Put the Standard in the pre heated water bath or heater block and press to start the colorimeter timer Just before the incubation time...
Страница 33: ...timer Just before the incubation time ends a beep will alert the user Put the sample in the cuvette holder and press The optical density of the Sample will be shown Press Return the sample to the inc...
Страница 34: ...elivery of product to end user premises 2 Free of all charges statement applies only in areas recognised by Sherwood as being serviced either directly by its own personnel or indirectly through person...
Страница 35: ...es purporting to be on behalf of Sherwood made by any person including distributors and representatives of Sherwood which are inconsistent or in conflict with the terms of this warranty including but...
Страница 36: ...TER GELATIN 600NM 252 22 001 FILTER GELATIN 710NM 252 26 001 FILTERS GELATIN SET OF 8 252 31 001 FILTER GELATIN 410NM 471 79 700 FILTER INTERFERENCE 430NM 471 79 800 FILTER HIGH SPEC 430NM 471 88 900...
Страница 37: ...ed levels of LDH are found after acute damage to the liver or the main muscles in cases of tumours known as lymphoma and in certain types of anaemia A form of this enzyme termed HBDH is raised in plas...
Страница 38: ...cuvette is placed in the heating device for a period which is usually between 10 30 minutes this is called the incubation period iv At the end of the measurement time the final absorbance OD is measur...
Страница 39: ...for Measure Time v 20 seconds before end of Measure Time buzzer sounds to remind user to prepare to replace cuvette into colorimeter vi At exact end of Measure Time cuvette is in light path and final...
Страница 40: ...r the test kit factor using then for each number option In this example we have entered a factor of 15 Once the factor has been entered the display changes to present the Absorbance measured and the c...
Страница 41: ...dard concentration using then for each number option In this example we have entered a concentration of 28 Insert the standard in the cuvette holder wait 10 seconds and then press The instrument will...
